Abstract:
:The smaller AB-cell of the 2-cell embryo of Tubifex divides 40 min later than the larger CD-cell. The present study was undertaken to elucidate those factors that are responsible for this cleavage asynchrony. An examination of chromosome cycles showed that the AB-cell entered M-phase 5 min later and spent about 35 min longer in the prometaphase portion of M-phase than did the CD-cell. Even if cultured in isolation, the length of M-phase of AB isolates continued to be comparable to that of intact AB-cells. When AB and CD nuclei were brought into a common cytoplasm, they entered M-phase simultaneously. The durations of M-phase portions from prometaphase through telophase were not altered even in a common cytoplasm, as long as the mitotic figures of AB and CD nuclei were separated from each other. Only when the mitotic spindles of both nuclei were united at at least one pole was the length of prometaphase of the AB nucleus significantly shortened. Whole-mount immunocytochemistry of microtubules revealed that asters were involved in mitotic spindle assembly in the CD-cell but not in the AB-cell. The fully formed spindle of the AB-cell was anastral and barrel-shaped, while that of the CD-cell possessed asters at its poles. The morphology of the spindle was not altered by changes in the cytoplasmic environments; that is, the spindle of AB nucleus was found to be anastral even in the presence of a CD-cell cytoplasm. These results suggest that unlike the case of other animals so far studied, cleavage asynchrony in the Tubifex embryo is brought about not only by cytoplasmic components but also by nucleus-associated factors.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Shimizu Tdoi
10.1006/dbio.1993.1123subject
Has Abstractpub_date
1993-05-01 00:00:00pages
191-204issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71123-1journal_volume
157pub_type
杂志文章abstract::The genetic and developmental bases for trait expression and variation in adults are largely unknown. One system in which genes and cell behaviors underlying adult traits can be elucidated is the larval-to-adult transformation of zebrafish, Danio rerio. Metamorphosis in this and many other teleost fishes resembles amp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00015-0
更新日期:2003-04-15 00:00:00
abstract::The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.12.022
更新日期:2007-04-01 00:00:00
abstract::Modulation of reactive oxygen species (ROS) plays a key role in signal transduction pathways. Selenoproteins act controlling the redox balance of the cell. We have studied how the alteration of the redox balance caused by patufet (selD(ptuf)), a null mutation in the Drosophila melanogaster selenophosphate synthetase 1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0389
更新日期:2001-10-01 00:00:00
abstract::Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.08.002
更新日期:2020-10-01 00:00:00
abstract::Eggs have developed their own strategies for early development. Amphibian, teleost fish, and ascidian eggs show cortical rotation and an accompanying structure, a cortical parallel microtubule (MT) array, during the one-cell embryonic stage. Cortical rotation is thought to relocate maternal deposits to a certain compa...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2018.11.019
更新日期:2019-04-15 00:00:00
abstract::Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences al...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90160-i
更新日期:1992-07-01 00:00:00
abstract::Nonmuscle myosin-II is a key motor protein that drives cell shape change and cell movement. Here, we analyze the function of nonmuscle myosin-II during Drosophila embryonic myogenesis. We find that nonmuscle myosin-II and the adhesion molecule, PS2 integrin, colocalize at the developing muscle termini. In the paradigm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0452
更新日期:2001-11-15 00:00:00
abstract::Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.04.017
更新日期:2019-08-15 00:00:00
abstract::Pax3 is expressed early during embryonic development in spatially restricted domains including limb muscle, neural crest, and neural tube. Pax3 functions at the nodal point in melanocyte stem cell differentiation, cardiogenesis and neurogenesis. Additionally Pax3 has been implicated in migration and differentiation of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.008
更新日期:2008-04-15 00:00:00
abstract::The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90121-i
更新日期:1991-06-01 00:00:00
abstract::Hes3 belongs to the Hes basic helix-loop-helix family of transcriptional repressors that play central roles in maintaining progenitor cells and regulating binary cell fate decisions in the embryo. During Xenopus laevis development, hes3 is expressed in the embryonic ectoderm in a horseshoe shape domain at the edge of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.07.011
更新日期:2018-10-01 00:00:00
abstract::Cyclic protein-2 (CP-2) is secreted in vitro in substantial amounts by mature rat Sertoli cells in intact Stage VI and Stage VII seminiferous tubules. This stage-dependent secretion has led us to postulate that the biosynthesis of this molecule is stimulated by germ cells at a specific state of development. In order t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90412-5
更新日期:1988-11-01 00:00:00
abstract::The contractile proteins of skeletal muscle are often represented by families of very similar isoforms. Protein isoforms can result from the differential expression of multigene families or from multiple transcripts from a single gene via alternative splicing. In many cases the regulatory mechanisms that determine the...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90067-q
更新日期:1992-12-01 00:00:00
abstract::Metamorphosis in Drosophila results from a hierarchy of ecdysone-induced gene expression initiated at the end of the third larval instar. A now classical model of this hierarchy was proposed based on observations of the activity of polytene chromosome "puffs" which distinguished "early" puffs as those directly induced...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9358
更新日期:1999-09-01 00:00:00
abstract::Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9140
更新日期:1999-03-01 00:00:00
abstract::FoxC, FoxF, FoxL1 and FoxQ1 genes have been shown to be clustered in some animal genomes, with mesendodermal expression hypothesised as a selective force maintaining cluster integrity. Hypotheses are, however, constrained by a lack of data from the Lophotrochozoa. Here we characterise members of the FoxC, FoxF, FoxL1 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.01.015
更新日期:2010-04-15 00:00:00
abstract::Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal developmen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90159-5
更新日期:1988-09-01 00:00:00
abstract::The MLH1 protein is required for normal meiosis in mice and its absence leads to failure in maintenance of pairing between bivalent chromosomes, abnormal meiotic division, and ensuing sterility in both sexes. In this study, we investigated whether failure to develop foci of MLH1 protein on chromosomes in prophase woul...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0708
更新日期:2002-09-01 00:00:00
abstract::The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing method. This method has been used in a variety of organisms and cell types over the past several years. However, using this technology for generating gene-edited animals involves a number of obstacles. One such obstacle is mosaicism,...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2018.10.008
更新日期:2019-01-15 00:00:00
abstract::Serotonin is a classical small-molecule neurotransmitter with known effects on developmental processes. Previous studies have shown a developmental role for serotonin in the fly peripheral nervous system. In this study, we show that serotonin can modulate the development of serotonergic varicosities within the fly cen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.07.025
更新日期:2005-10-01 00:00:00
abstract::The mammalian Cul4 genes, Cul4A and Cul4B, encode the scaffold components of the cullin-based E3 ubiquitin ligases. The two Cul4 genes are functionally redundant. Recent study indicated that mice expressing a truncated CUL4A that fails to interact with its functional partner ROC1 exhibit no developmental phenotype. We...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.01.028
更新日期:2011-04-15 00:00:00
abstract::The chick spinal cord can regenerate following injury until advanced developmental stages. It is conceivable that changes in stem/progenitor cell plasticity contribute to the loss of this capacity, which occurs around E13. We investigated the contribution of proliferation, phenotypic changes in radial glia progenitors...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.05.569
更新日期:2009-08-15 00:00:00
abstract::We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90313-r
更新日期:1991-11-01 00:00:00
abstract::The murine allantois will become the umbilical artery and vein of the chorioallantoic placenta. In previous studies, growth and differentiation of the allantois had been elucidated in whole embryos. In this study, the extent to which explanted allantoises grow and differentiate outside of the conceptus was investigate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0227
更新日期:2001-05-15 00:00:00
abstract::Glycosaminoglycans in the form of heparan sulfate proteoglycans (HSPG) and chondroitin sulfate proteoglycans (CSPG) are required for normal kidney organogenesis. The specific roles of HSPGs and CSPGs on ureteric bud (UB) branching morphogenesis are unclear, and past reports have obtained differing results. Here we emp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.029
更新日期:2004-08-15 00:00:00
abstract::Melanoblasts, the precursors of the pigment-producing cells of the skin and hair, are derived from the neural crest and migrate to the skin around 12 days of gestation in the mouse. In adult mice almost all the melanoblasts are confined to the hair follicles except for the epidermal layers of nonhairy skin. The recept...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9856
更新日期:2000-09-15 00:00:00
abstract::Biologists have long recognized that dramatic bending of a cell sheet may be driven by even modest shrinking of the apical sides of cells. Cell shape changes and tissue movements like these are at the core of many of the morphogenetic movements that shape animal form during development, driving processes such as gastr...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2009.09.009
更新日期:2010-05-01 00:00:00
abstract::In many non-mammalian vertebrates, adult dentitions result from cyclical rounds of tooth regeneration wherein simple unicuspid teeth are replaced by more complex forms. Therefore and by contrast to mammalian models, the numerical majority of vertebrate teeth develop shape during the process of replacement. Here, we ex...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.02.007
更新日期:2013-05-15 00:00:00
abstract::It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.007
更新日期:2015-10-01 00:00:00
abstract::Closure of the cranial neural tube depends on normal development of the head mesenchyme. Homozygous-mutant embryos for the ENU-induced open mind (opm) mutation exhibit exencephaly associated with defects in head mesenchyme development and dorsal-lateral hinge point formation. The head mesenchyme in opm mutant embryos ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.018
更新日期:2007-06-01 00:00:00