Abstract:
:Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Teng Y,Girard L,Ferreira HB,Sternberg PW,Emmons SWdoi
10.1016/j.ydbio.2004.09.012subject
Has Abstractpub_date
2004-12-15 00:00:00pages
476-92issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(04)00634-7journal_volume
276pub_type
杂志文章abstract::Previous work has documented the importance of BMPs in eye development. Loss-of-function studies in mice, with targeted deletions in either the Bmp7 or Bmp4 genes, have shown that these molecules are critical for early eye development. On the basis of the asymmetry in the dorsal-ventral expression patterns of several ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00115-x
更新日期:2003-04-01 00:00:00
abstract::To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.007
更新日期:2007-02-01 00:00:00
abstract::We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All thre...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8833
更新日期:1998-03-15 00:00:00
abstract::In Drosophila, the RNA helicase VASA (VAS) is required for both germ line formation and oocyte differentiation. While the murine VAS homologue is required for spermatogenesis, it is dispensable for germ line formation. The molecular basis for this apparently dual role of VAS in germ line ontogeny is, however, unclear....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.004
更新日期:2004-07-01 00:00:00
abstract::The earliest muscle in zebrafish arises from iterated sets of two to six cells in each somite, the muscle pioneers (MP). MP develop synchronously in young trunk myotomes adjacent to the notochord, precisely where the horizontal myoseptum will form. They elongate without cell fusion and differentiate hours earlier than...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90314-s
更新日期:1991-11-01 00:00:00
abstract::I report on the synthesis of fibronectin in the developing chick Müllerian duct mesenchymal cells. Before the differentiation of female chick Müllerian duct, the amount of fibronectin in the cells of the right duct is 44% lower than in the left duct. While after differentiation, the amount of fibronectin in the right ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90047-m
更新日期:1990-07-01 00:00:00
abstract::The migration and fate of cranial and vagal neural crest-derived progenitor cells (NCPCs) have been extensively studied; however, much less is known about sacral NCPCs particularly in regard to their distribution in the urogenital system. To construct a spatiotemporal map of NCPC migration pathways into the developing...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.04.011
更新日期:2017-09-01 00:00:00
abstract::In Caenorhabditis elegans, Wnt signaling regulates many asymmetric cell divisions. During embryogenesis, the C. elegans Dishevelled (Dsh) homolog, DSH-2, regulates asymmetric neuroblast division of the ABpl/rpppa blast cell. Dsh is a key intracellular component of both beta-catenin dependent and beta-catenin independe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.01.025
更新日期:2009-04-15 00:00:00
abstract::Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00255-0
更新日期:2003-08-15 00:00:00
abstract::Human MusTRD1alpha1 was isolated as a result of its ability to bind a critical element within the Troponin I slow upstream enhancer (TnIslow USE) and was predicted to be a regulator of slow fiber-specific genes. To test this hypothesis in vivo, we generated transgenic mice expressing hMusTRD1alpha1 in skeletal muscle....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.01.019
更新日期:2006-05-01 00:00:00
abstract::Arthropods, vertebrates, and annelids all have a segmented body. Our recent discovery of involvement of Notch-signalling in spider segmentation revived the discussion on the origin of segmented body plans and suggests the sharing of a common genetic program in a common ancestor. Here, we analysed the spider homologues...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.01.024
更新日期:2005-04-01 00:00:00
abstract::The enteric nervous system (ENS) forms from the neural crest-derived precursors that colonize the bowel before differentiating into a network of neurons and glia that control intestinal function. Retinoids are essential for normal ENS development, but the role of retinoic acid (RA) metabolism in development remains in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.06.021
更新日期:2013-09-01 00:00:00
abstract::Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90023-0
更新日期:1986-12-01 00:00:00
abstract::teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.028
更新日期:2007-07-01 00:00:00
abstract::POSH (Plenty of SH3s) has distinct roles as a scaffold for specific c-Jun N-terminal kinase (JNK) signaling modules and as an E3 ubiquitin ligase. The physiological function of POSH remains unclear, however, and its possible involvement in developmental processes motivated the present study wherein the Xenopus ortholo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.07.033
更新日期:2005-10-01 00:00:00
abstract::During development, cells craft an impressive array of actin-based structures, mediating events as diverse as cytokinesis, apical constriction, and cell migration. One challenge is to determine how cells regulate actin assembly and disassembly to carry out these cell behaviors. During Drosophila oogenesis diverse cell...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.030
更新日期:2009-09-01 00:00:00
abstract::Artificial extracellular matrices composed of collagen, glycosaminoglycans (GAG), proteoglycans (PG), plasma fibronectin (FN), and a hyaluronate-binding protein (HABP) have been prepared that morphologically resemble embryonic extracellular matrices in vivo at the light and electron microscope level. The effect of eac...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(85)90461-0
更新日期:1985-06-01 00:00:00
abstract::c-fms and c-kit are structurally related tyrosine kinase receptors for colony-stimulating factor-1 (CSF-1) and for steel factor (SF), respectively. The level of c-fms mRNA, like c-kit mRNA, increases during the maturation of oocytes and is an abundant maternal message found in the unfertilized oocyte. Following fertil...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90207-w
更新日期:1992-05-01 00:00:00
abstract::Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.009
更新日期:2007-02-15 00:00:00
abstract::Proper calcium channel and insulin signaling are essential for normal brain development. Leaner mice with a mutation in the P/Q-type voltage-gated calcium channel, Cacna1a, develop cerebellar atrophy and mutations in the homologous human gene are associated with increased migraine and seizure tendency. Similarly, abno...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.005
更新日期:2006-10-01 00:00:00
abstract::P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90202-t
更新日期:1990-04-01 00:00:00
abstract::Epidermal growth factor (EGF), a low-molecular-weight polypeptide (G. Carpenter and S. Cohen, 1979, Annu. Rev. Biochem. 48, 193-216), stimulates the proliferation and keratinisation of cultured embryonic epidermis (S. Cohen, 1965, Dev. Biol. 12, 394-407) and promotes epidermal growth, thickening, and keratinisation wh...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90243-9
更新日期:1983-12-01 00:00:00
abstract::Epithelial planar cell polarity (PCP) allows epithelial cells to coordinate their development to that of the tissue in which they reside. The mechanisms that impart PCP as well as effectors that execute the polarizing instructions are being sought in many tissues. We report that the epidermal epithelium of Drosophila ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.454
更新日期:2006-09-15 00:00:00
abstract::The location and movement of mammalian gut tissue progenitors, prior to the expression of tissue-specific genes, has been unknown, but this knowledge is essential to identify transitions that lead to cell type specification. To address this, we used vital dyes to label exposed anterior endoderm cells of early somite s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.01.003
更新日期:2005-04-01 00:00:00
abstract::Octopamine is an important neuroactive substance that modulates several physiological functions and behaviors of invertebrate species. Its biosynthesis involves two steps, one of which is catalyzed by Tyramine beta-hydroxylase enzyme (TBH). The Tbetah gene has been previously cloned from Drosophila melanogaster, and n...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.019
更新日期:2003-12-01 00:00:00
abstract::Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.01.010
更新日期:2019-03-15 00:00:00
abstract::The definitive endoderm forms during gastrulation and is rapidly transformed into the gut tube which is divided along the anterior-posterior axis into the foregut, midgut, and hindgut. Lineage tracing and genetic analysis have examined the origin of the definitive endoderm during gastrulation and demonstrated that the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.10.040
更新日期:2010-01-15 00:00:00
abstract::The individual blastomeres of the preimplantation mouse embryo become polarized during the 8-cell stage. Microvilli become restricted to the free surface of the embryo and this region of the membrane shows increased labeling with FITC-Con A and trinitrobenzenesulfonate (TNBS). Previous studies have shown that this pol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90204-6
更新日期:1986-04-01 00:00:00
abstract::Spermatogonial stem cells (SSCs) must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. This self-renewal vs. differentiation spermatogonial fate decision is critical for maintaining tissue homeostasis, as imbalances cau...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.08.004
更新日期:2015-11-01 00:00:00
abstract::The transition from maternal to zygotic gene expression in the mouse occurs in the 2-cell embryo. Previous studies in which DNA was injected into 2-cell embryos revealed that transcription promoters and origins of DNA replication are strongly repressed in cleavage stage embryos unless linked to an embryo-responsive en...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1160
更新日期:1995-06-01 00:00:00