Abstract：
:The protein content of various size follicles was measured in Fundulus heteroclitus and indicated four phases of increase relative to follicle volume: Phases I (previtellogenic; estimated to be less than 0.01 mg/mm3), II (vitellogenic; 0.20 mg/mm3), III (early maturation; 0.03 mg/mm3), and IV (late maturation; 0 mg/mm3). A pronounced and rapid size increase occurs during maturation due to hydration, but protein uptake, which was also documented cytologically, contributes to about 16% of the volume increment during early maturation. Protein incorporation appears to stop abruptly at the time of germinal vesicle breakdown, most likely reflecting an altered physiological state of the oocyte. SDS-polacrylamide gel electrophoretic patterns of various size follicles indicated that five major protein bands (molecular weights = 122, 103, 45, 26, and 20 k) accumulate during vitellogenesis and presumably are proteolytically derived from a 200-kDa vitellogenin precursor. During maturation, the 122- and 45-kDa proteins disappear and several new, lower molecular weight bands appear. Proteolysis of specific yolk proteins may thus help generate part of the osmotic pressure gradient required for water uptake during oocyte maturation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wallace RA,Selman Kdoi
10.1016/0012-1606(85)90106-xsubject
Has Abstractpub_date
1985-08-01 00:00:00pages
492-8issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(85)90106-Xjournal_volume
110pub_type
杂志文章abstract：:We have cloned a Caenorhabditis elegans homologue of the Drosophila gap gene hunchback (hb) and have designated it hbl-1 (hunchback-like). hbl-1 encodes a predicted 982-amino-acid protein, containing two putative zinc-finger domains similar to those of Drosophila Hunchback. The gene is transcribed embryonically, but u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9096
更新日期：1999-01-15 00:00:00
abstract：:Understanding the biology of somatic stem cells in self renewing tissues represents an exciting field of study, especially given the potential to harness these cells for tissue regeneration and repair in treating injury and disease. The mammalian cornea contains a population of basal epithelial stem cells involved in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.005
更新日期：2013-02-15 00:00:00
abstract：:Recent results have demonstrated the occurrence of mRNA cap methylation in the sea urchin embryo following fertilization. It has been suggested that this methylation event is responsible for the translational activation of maternal histone mRNAs in these embryos. We have used aphidicolin, an effective inhibitor of bot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90161-8
更新日期：1987-05-01 00:00:00
abstract：:The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 anima...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.11.010
更新日期：2015-02-01 00:00:00
abstract：:The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1040
更新日期：1995-02-01 00:00:00
abstract：:In neural plate stage Xenopus embryos, XlHbox 8 expression marks anterior endodermal cells fated to develop into pancreas/duodenum, and expression continues in adult pancreas in exocrine duct, acinar, and islet cells. Here, XlHbox 8 is used as a marker in experiments addressing the mechanisms of early endodermal patte...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1275
更新日期：1995-09-01 00:00:00
abstract：:An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8998
更新日期：1998-10-15 00:00:00
abstract：:Larvae homozygous for the recessive lethal allele without children(rgl) (woc(rgl)) fail to pupariate. Application of exogenous 20-hydroxyecdysone elicits puparium formation and pupation. Ecdysteroid titer measurements on mutant larvae show an endocrine deficiency in the brain-ring gland complex, which normally synthes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9811
更新日期：2000-10-01 00:00:00
abstract：:Intercellular communication mediated by gap junctions is important for tissue homeostasis in the avascular lens, and extensive areas of gap junctions form between fiber cells during fiber cell differentiation and lens development. We examined the role of the calcium-dependent cell adhesion molecule, N-cadherin, in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0237
更新日期：1996-10-10 00:00:00
abstract：:Selected stages of the developing chicken cornea have been examined for type VI collagen, employing monoclonal antibodies specific for this molecule. By immunofluorescence, the molecule is not detectable in 5 1/2 day corneas, a time at which the epithelial-derived, acellular primary stroma is the only corneal matrix p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90013-8
更新日期：1986-12-01 00:00:00
abstract：:Tabby is a mouse mutant characterized by deficient development of the ectodermal organs: teeth, hair, and a subset of glands. Ectodysplasin, the protein encoded by the Tabby gene, was recently identified as a novel TNF-like transmembrane protein but little is known about its function. We have examined the Tabby tooth ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9514
更新日期：1999-12-15 00:00:00
abstract：:T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.013
更新日期：2010-04-01 00:00:00
abstract：:We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca2+ release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.03.031
更新日期：2017-05-15 00:00:00
abstract：:MicroRNA attenuation of protein translation has emerged as an important regulator of mesenchymal cell differentiation into the osteoblast lineage. A compelling question is the extent to which miR biogenesis is obligatory for bone formation. Here we show conditional deletion of the Dicer enzyme in osteoprogenitors by C...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.008
更新日期：2010-04-01 00:00:00
abstract：:The shoot meristem generates all of the aerial structures of an adult plant. It is organized in three layers which produce the epidermis (L1 layer) and the subepidermal layers (L2 and L3). The origin of adult structures has previously been fate mapped to the primary meristem for L2 and L3 tissues. In this work we cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0112
更新日期：1996-05-01 00:00:00
abstract：:FoxC, FoxF, FoxL1 and FoxQ1 genes have been shown to be clustered in some animal genomes, with mesendodermal expression hypothesised as a selective force maintaining cluster integrity. Hypotheses are, however, constrained by a lack of data from the Lophotrochozoa. Here we characterise members of the FoxC, FoxF, FoxL1 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.015
更新日期：2010-04-15 00:00:00
abstract：:The Rho family of small GTPases has been implicated in many neurological disorders including mental retardation, but whether they are involved in primary microcephaly (microcephalia vera) is unknown. Here, we examine the role of Rac1 in mammalian neural progenitors and forebrain development by a conditional gene-targe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.10.023
更新日期：2009-01-01 00:00:00
abstract：:Mutations at the Drosophila melanogaster locus female sterile (1) homeotic (fs(1)h) result in segmental abnormalities including missing organs and homeotic transformations in the progeny of mutant mothers. Homeotic transformations are enhanced when the zygotes carry one of several third chromosome mutations, specifica...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90392-1
更新日期：1986-03-01 00:00:00
abstract：:Kidney development is a complex, little understood process based on inductive interactions and intricate epithelial and mesenchymal morphogenesis. Here, we report the use of subtractive hybridization to clone cDNAs expressed in early nephrogenesis. cDNA made from E14.5 mouse kidney was hybridized with adult mouse live...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1139
更新日期：1995-05-01 00:00:00
abstract：:To investigate the lineage relationships of neurons and astroglial cells early in central nervous system development, we have analyzed the progeny of neural plate cells in an amphibian embryo (Pleurodeles waltl). A fluorescent tracer, lysinated rhodamine-dextran, was iontophoretically injected into individual precurso...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1233
更新日期：1993-09-01 00:00:00
abstract：:Nicastrin is a recently identified member of high-molecular weight complexes containing presenilin. The Caenorhabditis elegans homolog of nicastrin, aph-2, was shown to be required for GLP-1/Notch signaling in the early embryo. In addition to the maternal-effect embryonic lethal phenotype, aph-2 mutant animals also di...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0486
更新日期：2001-12-15 00:00:00
abstract：:Ooplasmic segregation in the late interphase zygote of the leech Theromyzon trizonare is accomplished by reorganization of an ectoplasmic cytoskeleton formed by polar rings and meridional bands. The dynamic properties of this cytoskeleton were explored by time-lapse confocal and video microscopy. Cytoskeleton assembly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.023
更新日期：2004-07-01 00:00:00
abstract：:A complex regulatory cascade is required for normal cardiac development, and many aspects of this network are conserved from Drosophila to mammals. In Drosophila, the seven-up (svp) gene, an ortholog of the vertebrate chick ovalbumin upstream promoter transcription factors (COUP-TFI and II), is initially activated in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.025
更新日期：2007-02-15 00:00:00
abstract：:It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90230-e
更新日期：1992-05-01 00:00:00
abstract：:Major insulin gene transcription factors, such as PDX-1 or NeuroD1, have equally important roles in pancreatic development and the differentiation of pancreatic endocrine cells. Previously, we identified and cloned another critical insulin gene transcription factor MafA (RIPE3b1) and reported that other Maf factors we...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.028
更新日期：2006-05-15 00:00:00
abstract：:The Drosophila abnormal wing discs (awd) gene encodes the subunit of a protein that has nucleoside diphosphate kinase (NDP kinase) activity. Null mutations of the awd gene cause lethality after puparium formation. Larvae homozygous for such mutations have small imaginal discs, lymph glands, and brain lobes. Neither th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0184
更新日期：1996-08-01 00:00:00
abstract：:The endoderm of C. elegans arises entirely from a single progenitor cell, the E blastomere, whose identity is specified by GATA type transcription factors, including END-1. In response to an inductive interaction mediated through Wnt/MAP kinase signaling pathways, POP-1, a Lef/Tcf-type transcription factor, restricts ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.015
更新日期：2009-03-01 00:00:00
abstract：:In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.005
更新日期：2019-04-15 00:00:00
abstract：:The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90089-2
更新日期：1985-08-01 00:00:00
abstract：:In Dictyostelium discoideum cyclic AMP (cAMP) metabolism during macrocyst development, i.e., the sexual cycle of this organism, and in giant cells, i.e., fusion products from opposite mating-type cells, was investigated. The pattern of change in cAMP levels during macrocyst development differed considerably from that ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90104-0
更新日期：1984-08-01 00:00:00
