Abstract：
:The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 animals would significantly speed the characterization of these elements. We discovered that injecting 2-cell stage embryos with a plasmid bearing a ϕC31 integrase-targeted attB element and two dual β-globin HS4 insulators flanking a reporter transgene in opposite orientations relative to each other yielded persistent expression with sufficiently high penetrance for characterizing the activity of the promoter without having to coinject integrase RNA. Expression began appropriately during development and persisted into swimming tadpole stages without perturbing the expression of the cognate endogenous gene. Coinjected plasmids having the same elements but expressing different reporter proteins were reliably coexpressed within the same cells, providing a useful control for variations in injections between animals. To overcome the high propensity of these plasmids to undergo recombination, we developed a method for generating them using conventional cloning methods and DH5α cells for propagation. We conclude that this method offers a convenient and reliable way to evaluate the activity of cis-regulatory gene elements in the intact F0 embryo.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wang C,Szaro BGdoi
10.1016/j.ydbio.2014.11.010subject
Has Abstractpub_date
2015-02-01 00:00:00pages
11-23issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(14)00587-9journal_volume
398pub_type
杂志文章abstract：:The stability of sexual phenotype was examined in a single clone of Hydra oligactis males maintained at two culture temperatures, 18 and 22 degrees C. At these temperatures animals of this species do not reproduce sexually, but reproduce asexually by budding, and males and females are morphologically indistinguishable...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90311-8
更新日期：1986-10-01 00:00:00
abstract：:Activation of progenitor cells is crucial to promote tissue repair following injury in adult animals. In the context of successful limb regeneration following amputation, progenitor cells residing within the stump must re-enter the cell cycle to promote regrowth of the missing limb. We demonstrate that in axolotls, am...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.07.010
更新日期：2018-01-15 00:00:00
abstract：:We show that the intermediate filament protein nestin is expressed in myogenic cells and that multiple mechanisms regulate nestin expression at different stages of myogenesis. Cultured embryonic, fetal, and neonatal mouse limb myoblasts initially expressed nestin in the absence of the four muscle regulatory factors (M...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1248
更新日期：1994-09-01 00:00:00
abstract：:Stimulation of epithelial cell cycling by the neuropeptide head activator was analyzed in Hydra magnipapillata and compared with the action of head activator on bud formation and tentacle formation during head regeneration. The results obtained indicate that head activator treatment stimulates epithelial cell division...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8491
更新日期：1997-03-01 00:00:00
abstract：:Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.04.017
更新日期：2019-08-15 00:00:00
abstract：:Spontaneous germ cell death is a common cellular process in the mammalian testis, although the function of this process during spermatogenesis is unclear. An investigation was undertaken to determine whether p53 serves as a mechanism in germ cell quality control by causing spontaneous germ cell death. Using an annexin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9074
更新日期：1998-12-01 00:00:00
abstract：:Hand proteins are evolutionally conserved basic helix-loop-helix (bHLH) transcription factors implicated in development of neural crest-derived tissues, heart and limb. Hand1 is expressed in the distal (ventral) zone of the branchial arches, whereas the Hand2 expression domain extends ventrolaterally to occupy two-thi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.036
更新日期：2007-10-01 00:00:00
abstract：:A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprote...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.007
更新日期：2007-08-15 00:00:00
abstract：:The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Gl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0127
更新日期：2001-03-01 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:Integrins are alphabeta transmembrane receptors that function in key cellular processes, including cell adhesion, differentiation, and extracellular matrix deposition through interactions with extracellular, membrane, and cytoplasmic proteins. We previously identified and cloned a muscle beta1 integrin cytoplasmic bin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00304-x
更新日期：2003-09-01 00:00:00
abstract：:The dysgenetic lens (dyl) mouse mutant has mutations in Foxe3, which inactivate DNA binding by the encoded forkhead transcription factor. Here we confirm, by targeted inactivation, that Foxe3 mutations are responsible for the dyl phenotype, which include loss of lens epithelium; a small, cataractic lens; and failure o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.09.021
更新日期：2007-02-01 00:00:00
abstract：:Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expres...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.020
更新日期：2007-01-15 00:00:00
abstract：:Initial studies suggested that spatial organization of the putative polar body contractile ring was determined by the peripheral aster in Spisula [Biol. Bull. 205 (2003) 192]. Here we report detailed supporting observations, including testing of aster and ring function with inhibitors. The metaphase peripheral aster w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.033
更新日期：2004-05-15 00:00:00
abstract：:In vertebrates, nodal-related genes are crucial for specifying mesendodermal cell fates. Six nodal-related genes have been identified in Xenopus, but only one, nodal, has been identified in the mouse. The Xenopus nodal-related gene 3 (Xnr3), however, lacks the mesoderm-inducing activity of the other five nodal-related...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.09.015
更新日期：2004-01-01 00:00:00
abstract：:Developmental patterning and growth of the vertebrate digestive and respiratory tracts requires interactions between the epithelial endoderm and adjacent mesoderm. The esophagus is a specialized structure that connects the digestive and respiratory systems and its normal development is critical for both. Shh signaling...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.08.024
更新日期：2011-11-15 00:00:00
abstract：:VegT is an essential maternal regulator of germ layer specification in Xenopus. The localization of VegT mRNA to the vegetal cortex of the oocyte during oogenesis ensures its inheritance by vegetal and not animal cells, and directs the differentiation of vegetal cells into endoderm. Similarly localized mRNAs, Vg1 and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0495
更新日期：2001-12-15 00:00:00
abstract：:Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.003
更新日期：2017-06-15 00:00:00
abstract：:The forkhead genes encode a transcription factor involved in embryogenesis and pattern formation in multicellular organisms. They are mammalian transcriptional regulators that bind DNA as a monomer through their forkhead domain. The Foxf2 (LUN) mRNA is expressed in the mesenchyme directly adjacent to the ectoderm-deri...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00176-3
更新日期：2003-07-01 00:00:00
abstract：:In Dictyostelium discoideum cyclic AMP (cAMP) metabolism during macrocyst development, i.e., the sexual cycle of this organism, and in giant cells, i.e., fusion products from opposite mating-type cells, was investigated. The pattern of change in cAMP levels during macrocyst development differed considerably from that ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90104-0
更新日期：1984-08-01 00:00:00
abstract：:All metazoan genomes encode multiple RAS GTPase activating proteins (RasGAPs) that negatively regulate the conserved RAS/MAPK signaling pathway. In mammals, several RasGAPs exhibit tumor suppressor activity by preventing excess RAS signal transduction. We have identified gap-3 as the to date missing Caenorhabditiseleg...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.08.026
更新日期：2008-11-15 00:00:00
abstract：:In the small-optic-lobes (sol) and sine oculis (so) mutants of Drosophila melanogaster extensive cell death occurs in the optic lobes during the first half of pupal development. Gynandromorph flies show that the sol mutation acts primarily on cells of the medulla cortex. Degeneration of medullar ganglion cells occurs ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90050-2
更新日期：1984-07-01 00:00:00
abstract：:Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.017
更新日期：2014-09-01 00:00:00
abstract：:The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1002
更新日期：1993-01-01 00:00:00
abstract：:Skeletal muscles of the avian limb are derived from mononucleated myogenic precursor cells (myoblasts) that migrate into the somatopleural mesoderm of the developing limb bud from the ventrolateral dermomyotome of limb adjacent somites. In the present study, we utilized replication-deficient lacZ-encoding retroviruses...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00028-3
更新日期：2003-01-15 00:00:00
abstract：:The self-organizing tissue-based approach coupled with induced pluripotent stem (iPS) cell technology is evolving as a promising field for designing organoids in culture and is expected to achieve valuable practical outcomes in regenerative medicine and drug development. Organoids show properties of functional organs ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.06.036
更新日期：2016-12-15 00:00:00
abstract：:Dorsoventral specification of the zebrafish gastrula is governed by the functions of the dorsal shield, a region of the embryo functionally analogous to the amphibian Spemann organizer. We report that the bozozok locus encodes the transcription factor nieuwkoid/dharma, a homeobox gene with non-cell-autonomous organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9479
更新日期：1999-11-15 00:00:00
abstract：:The organization of the embryonic neural plate requires coordination of multiple signal transduction pathways, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs), and WNTs. Many studies have suggested that a critical component of this process is the patterning of posterior neural tissues by...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.019
更新日期：2009-09-01 00:00:00
abstract：:Stable subdivision of Drosophila limbs into Anterior (A) and Posterior (P) compartments is a consequence of asymmetric signaling by Hedgehog (Hh) from P to A cells. The activity of the homeodomain protein Engrailed (En) in P cells has been reported to help to generate this asymmetry by inducing the expression of hedge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.006
更新日期：2009-03-15 00:00:00
abstract：:The elongation of embryo and tissue is a key morphogenetic event in embryogenesis and organogenesis. Notochord, a typical chordate organ, undergoes elongation to perform its regulatory roles and to form the structural support in the embryo. Notochord elongation is morphologically similar across all chordates, but asci...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.009
更新日期：2019-04-15 00:00:00
