Abstract：
:MicroRNA attenuation of protein translation has emerged as an important regulator of mesenchymal cell differentiation into the osteoblast lineage. A compelling question is the extent to which miR biogenesis is obligatory for bone formation. Here we show conditional deletion of the Dicer enzyme in osteoprogenitors by Col1a1-Cre compromised fetal survival after E14.5. A mechanism was associated with the post-commitment stage of osteoblastogenesis, demonstrated by impaired ECM mineralization and reduced expression of mature osteoblast markers during differentiation of mesenchymal cells of ex vivo deleted Dicer(c/c). In contrast, in vivo excision of Dicer by Osteocalcin-Cre in mature osteoblasts generated a viable mouse with a perinatal phenotype of delayed bone mineralization which was resolved by 1 month. However, a second phenotype of significantly increased bone mass developed by 2 months, which continued up to 8 months in long bones and vertebrae, but not calvariae. Cortical bone width and trabecular thickness in Dicer(Deltaoc/Deltaoc) was twice that of Dicer(c/c) controls. Normal cell and tissue organization was observed. Expression of osteoblast and osteoclast markers demonstrated increased coupled activity of both cell types. We propose that Dicer generated miRs are essential for two periods of bone formation, to promote osteoblast differentiation before birth, and control bone accrual in the adult.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Gaur T,Hussain S,Mudhasani R,Parulkar I,Colby JL,Frederick D,Kream BE,van Wijnen AJ,Stein JL,Stein GS,Jones SN,Lian JBdoi
10.1016/j.ydbio.2010.01.008subject
Has Abstractpub_date
2010-04-01 00:00:00pages
10-21issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(10)00015-1journal_volume
340pub_type
杂志文章abstract：:Egfr/Ras signaling promotes vein cell fate specification in the developing Drosophila wing. While the importance of Ras signaling in vein determination has been extensively documented, the mechanisms linking Ras activity to vein differentiation remain unclear. We found that Ras signaling regulates both the levels and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.003
更新日期：2007-11-01 00:00:00
abstract：:The spe-9 gene is required for fertility in Caenorhabditis elegans and encodes a sperm transmembrane protein with an extracellular domain (ECD) that contains 10 epidermal growth factor (EGF) repeats. Deletion analysis reveals that the EGF repeats and the transmembrane domain are required for fertilization. In contrast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.014
更新日期：2004-08-15 00:00:00
abstract：:The insect head is composed of several segments. During embryonic development, the segments fuse to form a rigid head capsule where obvious segmental boundaries are lacking. Hence, the assignment of regions of the insect head to specific segments is hampered, especially with respect to dorsal (vertex) and lateral (gen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.010
更新日期：2010-02-01 00:00:00
abstract：:c-fms and c-kit are structurally related tyrosine kinase receptors for colony-stimulating factor-1 (CSF-1) and for steel factor (SF), respectively. The level of c-fms mRNA, like c-kit mRNA, increases during the maturation of oocytes and is an abundant maternal message found in the unfertilized oocyte. Following fertil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90207-w
更新日期：1992-05-01 00:00:00
abstract：:In Drosophila, the RNA helicase VASA (VAS) is required for both germ line formation and oocyte differentiation. While the murine VAS homologue is required for spermatogenesis, it is dispensable for germ line formation. The molecular basis for this apparently dual role of VAS in germ line ontogeny is, however, unclear....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.004
更新日期：2004-07-01 00:00:00
abstract：:It has been posited that animal development evolved from pre-existing mechanisms for regulating cell differentiation in the single celled and colonial ancestors of animals. Although the progenitors of animals cannot be studied directly, insights into their cell biology may be gleaned from comparisons between animals a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.06.003
更新日期：2011-09-01 00:00:00
abstract：:The origin of the avian cardiovascular system from the primitive streak has been mapped by the construction of quail/chick transplantation chimeras, the use of QH-1 (an antiquail endothelial/endocardial cell marker), and injections of a vital fluorescent dye (DiI) into the primitive streak. Our studies reveal that the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1276
更新日期：1993-10-01 00:00:00
abstract：:It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.07.007
更新日期：2015-10-01 00:00:00
abstract：:From July 19-24, 2015, 169 clinicians and basic scientists gathered in the vertiginous heights of Snowmass, Colorado (2502 m) for the fourth FASEB summer research conference on the 'Biology of Cilia and Flagella'. Organizers Maureen Barr (Rutgers University), Iain Drummond (Massachusetts General Hospital/Harvard Medic...
journal_title：Developmental biology
pub_type：
doi：10.1016/j.ydbio.2015.10.030
更新日期：2016-01-15 00:00:00
abstract：:During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein dis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.02.019
更新日期：2010-05-01 00:00:00
abstract：:Pole cell formation in embryos of the parthenogenetic midge, Smittia sp., can be delayed or inhibited by irradiation of the posterior egg pole with ultraviolet light (uv). This leaves the schedule of nuclear divisions and chromosome eliminations virtually unaffected. However, uv irradiation delays the precocious migra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90236-1
更新日期：1983-12-01 00:00:00
abstract：:The protein content of various size follicles was measured in Fundulus heteroclitus and indicated four phases of increase relative to follicle volume: Phases I (previtellogenic; estimated to be less than 0.01 mg/mm3), II (vitellogenic; 0.20 mg/mm3), III (early maturation; 0.03 mg/mm3), and IV (late maturation; 0 mg/mm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90106-x
更新日期：1985-08-01 00:00:00
abstract：:Mammalian eggs remain arrested at metaphase of the second meiotic division (metII) for an indeterminate time before fertilization. During this period, which can last several hours, the continued attachment of sister chromatids is thought to be achieved by inhibition of the protease separase. Separase is known to be in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.06.036
更新日期：2008-09-15 00:00:00
abstract：:Cellular dedifferentiation underlies topical issues in biology such as regeneration and nuclear cloning and has common features in plants and animals. In plants, this process characterizes the transition of differentiated leaf cells to protoplasts (plant cells devoid of cell walls) and is accompanied by global chromat...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.023
更新日期：2007-06-15 00:00:00
abstract：:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90291-n
更新日期：1992-02-01 00:00:00
abstract：:We examined the development of epithelial Na+ conductance in 6- and 7-day post coitus (p.c.) preimplantation rabbit embryos using the whole-cell patch-clamp technique on dissociated rabbit trophectodermal cells and by immunocytochemical localization using a polyclonal antibody directed against subunits of an apical ep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90289-f
更新日期：1991-10-01 00:00:00
abstract：:The transcription factors, GATA4, 5 and 6, recognize the same DNA sequence and are all expressed in the developing myocardium. However, knockout studies in the mouse have indicated that none of them are absolutely required for the specification of the myocardium. Here we present evidence for redundancy in this family ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.018
更新日期：2007-11-15 00:00:00
abstract：:We present evidence to show that differentiation in wing cells to produce hairs is synchronous over the distal 90% of the wing surface (approximately 28,000 cells). In spite of this synchrony within such a large area a temporal gradient exists between zones (in general anterior to posterior) on the animal surface with...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90047-7
更新日期：1983-02-01 00:00:00
abstract：:A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90193-x
更新日期：1987-06-01 00:00:00
abstract：:Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lap...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.03.016
更新日期：2019-06-15 00:00:00
abstract：:TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.03.045
更新日期：2008-08-01 00:00:00
abstract：:Superior cervical ganglia of postnatal mice with a targeted disruption of the gene for neurotrophin-3 have 50% fewer neurons than those of wild-type mice. In culture, neurotrophin-3 increases the survival of proliferating sympathetic precursors. Both precursor death (W. ElShamy et al., 1996, Development 122, 491-500) ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9269
更新日期：1999-06-15 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:The segregation of founder cells from the somatic mesoderm is a prerequisite for the formation of body wall muscles in the Drosophila embryo. The myogenic basic helix-loop-helix protein, Nautilus (Nau), is expressed in a subset of these founder cells in medial and lateral positions in the somatic mesoderm. Mutations i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.9987
更新日期：1996-05-25 00:00:00
abstract：:In Caenorhabditis elegans, Wnt signaling regulates many asymmetric cell divisions. During embryogenesis, the C. elegans Dishevelled (Dsh) homolog, DSH-2, regulates asymmetric neuroblast division of the ABpl/rpppa blast cell. Dsh is a key intracellular component of both beta-catenin dependent and beta-catenin independe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.025
更新日期：2009-04-15 00:00:00
abstract：:Axon outgrowth between the spinal cord and the hindlimb of the chick embryo is constrained by three tissues that border axon pathways. Growth cones turn to avoid the posterior sclerotome, perinotochordal mesenchyme, and pelvic girdle precursor during normal development and after experimental manipulation. We wanted to...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(05)80017-x
更新日期：1991-09-01 00:00:00
abstract：:Zygotic Wnt signaling has been shown to be involved in dorsoventral mesodermal patterning in Xenopus embryos, but how it regulates different myogenic gene expression in the lateral mesodermal domains is not clear. Here, we use transient exposure of embryos or explants to lithium, which mimics Wnt/beta-catenin signalin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0633
更新日期：2002-05-01 00:00:00
abstract：:Acute myeloid leukemia (AML) is a complex malignancy with poor prognosis. Several genetic lesions can lead to the disease. One of these corresponds to the NUP98-HOXA9 (NA9) translocation that fuses sequences encoding the N-terminal part of NUP98 to those encoding the DNA-binding domain of HOXA9. Despite several studie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.003
更新日期：2017-01-01 00:00:00
abstract：:The transcription factor Pax6 plays a pivotal role in eye development, as eye morphogenesis is arrested at a primitive optic vesicle stage in homozygous Pax6 mutant mouse embryos. The arrested optic vesicle development has led to the assumption that cellular differentiation programs are unable to initiate. Contrary to...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.12.018
更新日期：2005-03-15 00:00:00
abstract：:The source of neurite outgrowth in explant cultures of normal adult Apteronotus spinal cord was examined. Explants which contained the central region of spinal cord, including ependyma, showed neurite outgrowth in culture. Explants which did not contain ependyma showed no neurite outgrowth. It is concluded that the ep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90062-5
更新日期：1987-02-01 00:00:00
