Abstract:
:We examined the structure, expression, and possible functions of a nanos homologue during gametogenesis and blastogenesis in the colonial ascidian Botryllus primigenus. An approximately 1.3-kb-long cDNA was cloned; it was termed BpNos since the deduced amino acid sequence (288 aa) contained 2 Nanos-like CCHC zinc finger motifs. Immature and mature male germ cells expressed BpNos most strongly, while loose aggregates of hemoblasts, multipotent epithelial cells (in developing buds) and a few coelomic cells in the hemocoel and tunic vessels weakly expressed BpNos. No signals were detected from female germ cells. To determine possible functions of BpNos, B. primigenus colonies were injected with BpNos short interfering (si)RNA. Buds developed normally, showing that BpNos plays a limited role in B. primigenus blastogenesis. However, the developing buds possessed no spermatogonia and spermatocytes in the testes, although oocytes developed normally. In the knockdown colonies, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay (TUNEL)-positive male germ cells were observed, suggesting that BpNos siRNA treatment might induce apoptosis. In conclusion, BpNos is a weak marker of germline precursor cells and multipotent somatic epithelial cells but a strong marker of spermatogonia and spermatocytes. A major function of BpNos may be the maintenance of male germline cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Sunanaga T,Satoh M,Kawamura Kdoi
10.1016/j.ydbio.2008.08.023subject
Has Abstractpub_date
2008-12-01 00:00:00pages
31-40issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(08)01152-4journal_volume
324pub_type
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