Abstract:
:Full-grown Xenopus oocytes, Stage VI (1200-1300 microns), undergo meiotic maturation when exposed to progesterone. Smaller stage IV (800 microns) and stage V (1000 microns) oocytes remain in prophase arrest when exposed to this steroid. The larger stage VI oocytes undergo an intracellular alkalization from 7.2 to 7.6, a six- to eightfold increase in the phosphorylation of the 40 S ribosomal protein S-6, and a two- to threefold increase in total protein synthesis when exposed to progesterone. It was found that 800- to 1000-microns oocytes do not undergo these physiological changes when exposed to progesterone. This lack of response could explain the failure of small oocytes to undergo germinal vesicle breakdown (GVBD). However, when stage IV and V oocytes were artificially alkalized to a pHi of 7.6 by the weak bases, trimethylamine, procaine, or methylamine, S-6 phosphorylation was stimulated four- to sixfold and protein synthesis was stimulated two- to threefold, but they still did not undergo GVBD. Stage IV and V oocytes are able to amplify MPF injected into their cytoplasm and undergo GVBD. Thus, 800- to 1000-microns oocytes appear to contain a store of inactive MPF in their cytoplasm. It seems that an additional physiological parameter(s), that is unique to steroid-treated stage VI oocytes, is responsible for activating this MPF which induces GVBD.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wasserman WJ,Houle JG,Samuel Ddoi
10.1016/0012-1606(84)90288-4subject
Has Abstractpub_date
1984-10-01 00:00:00pages
315-24issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(84)90288-4journal_volume
105pub_type
杂志文章abstract::During early development, GATA factors have been shown to be important for key events of coronary vasculogenesis, including formation of the epicardium. Myocardial GATA factors are required for coronary vascular (CV) formation; however, the role of epicardial localized GATAs in this process has not been addressed. The...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.12.033
更新日期:2014-02-01 00:00:00
abstract::The vertebrate intestinal epithelium is renewed continuously from stem cells at the base of the crypt in mammals or base of the fold in fish over the life of the organism. As stem cells divide, newly formed epithelial cells make an initial choice between a secretory or enterocyte fate. This choice has previously been ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.013
更新日期:2013-04-15 00:00:00
abstract::Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct regions, exhibiting differences in m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.06.004
更新日期:2018-09-15 00:00:00
abstract::The prostate undergoes branching morphogenesis dependent on paracrine interactions between the prostatic epithelium and the urogenital mesenchyme. To identify cell-surface molecules that function in this process, monoclonal antibodies raised against epithelial cell-surface antigens were screened for antigen expression...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0223
更新日期:2001-05-01 00:00:00
abstract::The iris sphincter muscle consists of striated muscle fibers in the adult chicken. The ontogenetic development of this muscle has been studied by immunocytochemistry, from Embryonic Day 7 to the time of hatching. The time course of expression of specific markers of either smooth or striated muscle, i.e., smooth and sk...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1254
更新日期:1993-10-01 00:00:00
abstract::Joint and skeletal development is highly regulated by extracellular matrix (ECM) proteoglycans, of which chondroitin sulfate proteoglycans (CSPGs) are a major class. Despite the requirement of joint CSPGs for skeletal flexibility and structure, relatively little is understood regarding their role in establishing joint...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.01.005
更新日期:2012-03-15 00:00:00
abstract::Egg yolk phosvitin is one of the most highly phosphorylated extracellular matrix proteins known in nature with unique physico-chemical properties deemed to be critical during ex-vivo egg embryo development. We have utilized our unique live mouse calvarial bone organ culture models under conditions which dissociates th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.06.005
更新日期:2013-09-01 00:00:00
abstract::The Drosophila segment polarity gene known as shaggy or zeste white 3 encodes a ubiquitously expressed serine-threonine protein kinase which is critical for a number of important developmental processes. In the developing wing blade, clones of cells lacking the normal shaggy-zeste white 3 product form dense tufts of m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1081
更新日期:1994-03-01 00:00:00
abstract::We describe the identification, biochemical characterisation, and mutation of a novel mouse gene: Sp5. Sp5 encodes a protein having a C-terminal C(2)H(2) zinc finger domain closely related to that of the transcription factor Sp1. In vitro, DNA binding studies show that it binds to the GC box, a DNA motif present in th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9878
更新日期:2000-11-15 00:00:00
abstract::Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced metazoan transcripts, yet inh...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.09.010
更新日期:2020-01-01 00:00:00
abstract::Mesodermal cell migration during amphibian gastrulation is dependent on cellular interactions with fibronectin. One mechanism whereby cells bind fibronectin is through alpha v-containing integrin heterodimers. In order to investigate the role of alpha v in amphibian gastrulation, we have cloned the Pleurodeles homolog...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1212
更新日期:1995-08-01 00:00:00
abstract::Several Strongylocentrotus purpuratus gene cis-regulatory regions contain asymmetric C4 sequences which are core elements of target sites for a specific DNA-protein interaction. Blastula stage nuclear extract contains five proteins which specifically bind to these target sites, resulting in a characteristic pattern of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1181
更新日期:1995-06-01 00:00:00
abstract::Successful fertilization requires that sperm are activated prior to contacting an oocyte. In C. elegans, this activation process, called spermiogenesis, transforms round immobile spermatids into motile, fertilization-competent spermatozoa. We describe the phenotypic and genetic characterization of spe-43, a new compon...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.02.013
更新日期:2018-04-15 00:00:00
abstract::Lumenogenesis of small seamless tubes occurs through intracellular membrane growth and directed vesicle fusion events. Within the Caenorhabditis elegans excretory cell, which forms seamless intracellular tubes (canals) that mediate osmoregulation, lumens grow in length and diameter when vesicles fuse with the expandin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.07.019
更新日期:2014-10-01 00:00:00
abstract::The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.12.022
更新日期:2007-04-01 00:00:00
abstract::Egfr/Ras signaling promotes vein cell fate specification in the developing Drosophila wing. While the importance of Ras signaling in vein determination has been extensively documented, the mechanisms linking Ras activity to vein differentiation remain unclear. We found that Ras signaling regulates both the levels and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.08.003
更新日期:2007-11-01 00:00:00
abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
abstract::The regeneration of lost body parts is a fascinating phenomenon exhibited by some multicellular organisms. In social amoebae, such as Dictyostelium discoideum, the pseudoplasmodium is a temporary migratory multicellular structure with high regeneration ability. It consists of future stalk cells (prestalk cells) at the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.09.014
更新日期:2020-01-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have been recently recognized as a major class of regulators in mammalian systems. LncRNAs function by diverse and heterogeneous mechanisms in gene regulation, and are key contributors to development, neurological disorders, and cancer. This emerging importance of lncRNAs, along with rec...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.035
更新日期:2013-04-15 00:00:00
abstract::Ligands of the transforming growth factor beta (TGFbeta) superfamily, like Nodal and bone morphogenetic protein (BMP), are pivotal to establish left-right (LR) asymmetry in vertebrates. However, the receptors mediating this process are unknown. Here we identified two new type II receptors for BMPs in zebrafish termed ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.038
更新日期:2008-03-01 00:00:00
abstract::Thyroid hormone (TH) induces dramatic skeletal and tissue remodeling of the anuran head and body at metamorphosis. The expression pattern of TH up-regulated genes has been correlated with tissues that either grow or resorb at metamorphosis. Whereas the expression of the thyroid hormone receptors in Xenopus laevis tadp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8975
更新日期:1998-11-01 00:00:00
abstract::The mouse Otlx2 gene is a new member of the paired-like family of homeobox genes whose human homologue, RIEG, is involved in Rieger syndrome, an autosomal-dominant disorder. One of the cardinal features of Rieger syndrome is dental hypoplasia, indicating that Otlx2/RIEG activity is essential for normal tooth developme...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8672
更新日期:1997-09-15 00:00:00
abstract::We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All thre...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8833
更新日期:1998-03-15 00:00:00
abstract::Monoclonal antibodies have been generated to determine the fate of sperm-specific surface components of the sea urchin, Arbacia punctulata, subsequent to gamete fusion. Monoclonal antibody-7 (MAB-7) reacted with two polypeptide bands having apparent molecular masses of 33 and 35 kDa derived from the sperm plasma membr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80036-3
更新日期:1989-01-01 00:00:00
abstract::LIM-Kinase (LIMK), including LIMK1 and LIMK2, is the only known catalytic protein among LIM-family molecules. It is well known that LIMK phosphorylates and inactivates cofilin, an actin-depolymerizing factor regulating actin reorganization, while in vivo functions have remained to be elucidated. In the present study, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0512
更新日期:2002-01-15 00:00:00
abstract::The nervous system development of the sea cucumber Stichopus japonicus was investigated to explore the development of the bilateral larval nervous system into the pentaradial adult form typical of echinoderms. The first nerve cells were detected in the apical region of epidermis in the late gastrula. In the auriculari...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.038
更新日期:2006-04-01 00:00:00
abstract::The protein content of various size follicles was measured in Fundulus heteroclitus and indicated four phases of increase relative to follicle volume: Phases I (previtellogenic; estimated to be less than 0.01 mg/mm3), II (vitellogenic; 0.20 mg/mm3), III (early maturation; 0.03 mg/mm3), and IV (late maturation; 0 mg/mm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90106-x
更新日期:1985-08-01 00:00:00
abstract::Much progress has been made in elucidating the molecular networks required for specifying retinal cells, including photoreceptors, but the downstream mechanisms that maintain identity and regulate differentiation remain poorly understood. Here, we report that the transcription factor Glass has a dual role in establish...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.04.012
更新日期:2016-06-01 00:00:00
abstract::Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm bindin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90191-6
更新日期:1987-06-01 00:00:00
abstract::Members of the fibroblast growth factor (FGF) family of peptide growth factors are widely expressed in the germ layer derivatives during gastrulation and early organogenesis of the mouse. We have investigated the effect of administering recombinant FGF-4 in the late-primitive streak stage embryo to test if the pattern...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9663
更新日期:2000-05-01 00:00:00