Abstract:
:Primary cultures of cardiac myocytes from newborn normal and genetically cardiomyopathic (strain UM-X7.1) hamsters were analyzed by electron microscopy and immunofluorescent staining for myosin, actin, tropomyosin, and alpha-actinin. Antibody staining of these contractile proteins demonstrates that both normal and cardiomyopathic (CM) myocytes contain prominent myofibrils after 3 days in culture, although the CM myofibrils are disarrayed and not aligned as those in normal cells. The disarray becomes even more pronounced in CM cells after 5 days in culture. The immunofluorescent staining patterns of individual myofibrils in normal and CM cells were similar for myosin, actin, and tropomyosin. However, alpha-actinin staining reveals that the CM myofibrils have abnormally wide and irregularly shaped Z bands. Electron microscopy confirms the irregular Z-band appearance as well as the myofibril disarray. Thus, CM cardiomyocytes clearly show an aberrant pattern of myofibril structure and organization in culture.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Lemanski LF,Tu ZHdoi
10.1016/0012-1606(83)90091-xsubject
Has Abstractpub_date
1983-06-01 00:00:00pages
338-48issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90091-Xjournal_volume
97pub_type
杂志文章abstract::In the mouse embryo, the onset of zygotic transcription occurs at the end of the first cell cycle, upon completion of DNA replication. We show that the nonhistone chromosomal protein HMG-I, whose translocation into the pronuclei of one-cell embryos is linked to this first round of DNA synthesis, plays a critical role ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9668
更新日期:2000-05-15 00:00:00
abstract::Previous study of the development of the levator ani muscle (LA) revealed that the sex difference in muscle fiber number is under the control of testosterone during the perinatal period. In this study, the development of the LA muscle was examined in both sexes to test the hypothesis that testosterone action determine...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1070
更新日期:1994-03-01 00:00:00
abstract::A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8504
更新日期:1997-04-01 00:00:00
abstract::The murine cpk mouse develops a rapid-onset polycystic kidney disease (PKD) with many similarities to human PKD. During kidney development, the transcription factor Pax2 is required for the specification and differentiation of the renal epithelium. In humans, Pax2 is also expressed in juvenile cystic kidneys where it ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9618
更新日期:2000-03-15 00:00:00
abstract::The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90241-7
更新日期:1987-04-01 00:00:00
abstract::The transforming growth factor-beta (TGF-beta) superfamily contains a variety of growth factors which all share common sequence elements and structural motifs. These proteins are known to exert a wide spectrum of biological responses on a large variety of cell types in both vertebrates and invertebrates. Many of them ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-15 00:00:00
abstract::A monoclonal antibody (MAb No. 25-16), raised against purified cortical secretory vesicles (CVs) from the eggs of Strongylocentrotus purpuratus, has been used to identify a previously uncharacterized CV-derived polypeptide component of the sea urchin fertilization envelope (FE). MAb No. 25-16, an IgG1, bound to a grou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90210-8
更新日期:1989-03-01 00:00:00
abstract::We have reported previously that sequence-specific adenylations and deadenylations accompany changes in the translation of maternal mRNA following fertilization of Spisula oocytes (E.T. Rosenthal, T.R. Tansey, and J.V. Ruderman, 1983, J. Mol. Biol. 166, 309-327). The data presented here confirm and extend those observ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90155-2
更新日期:1987-05-01 00:00:00
abstract::Cellular dedifferentiation underlies topical issues in biology such as regeneration and nuclear cloning and has common features in plants and animals. In plants, this process characterizes the transition of differentiated leaf cells to protoplasts (plant cells devoid of cell walls) and is accompanied by global chromat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.023
更新日期:2007-06-15 00:00:00
abstract::Mechanisms coupling growth and metabolism are conserved in Drosophila and mammals. In metazoans, such coupling is achieved across tissue scales through the regulated secretion of chemical messengers such as insulin that control the metabolism and growth of cells. Although the regulated secretion of Insulin like peptid...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.12.008
更新日期:2018-02-01 00:00:00
abstract::The shoot meristem generates all of the aerial structures of an adult plant. It is organized in three layers which produce the epidermis (L1 layer) and the subepidermal layers (L2 and L3). The origin of adult structures has previously been fate mapped to the primary meristem for L2 and L3 tissues. In this work we cons...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0112
更新日期:1996-05-01 00:00:00
abstract::Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90392-v
更新日期:1990-10-01 00:00:00
abstract::John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2016.11.005
更新日期:2017-09-15 00:00:00
abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1076
更新日期:1993-03-01 00:00:00
abstract::Zygotic gene activation is essential for development beyond the 2-cell stage in the preimplantation mouse embryo. Based on alpha-amanitin-sensitive BrUTP incorporation, transcription initiates in the 1-cell embryo and a major reprogramming of gene expression driven by newly expressed genes is prominently observed duri...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.03.038
更新日期:2005-07-01 00:00:00
abstract::Cerebral and spinal vascular systems are organized individually, and they then conjugate at their border, through the integration of basilar artery and vertebral arteries. Zebrafish (Danio rerio) is an ideal organism for studying early vascular development, and the precise procedure of cranial and truncal vascular for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.015
更新日期:2015-10-01 00:00:00
abstract::Fertilization-related sperm mitochondrial movement occurs at a rate comparable to other actin-myosin-driven movements and is inhibited by cytochalasin B and N-ethyl maleimide in Ascidia ceratodes sperm. F-actin was demonstrated in the tails and mitochondria using NBD-phallacidin fluorescence. Both actin and myosin wer...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90229-x
更新日期:1984-12-01 00:00:00
abstract::Previous studies have shown that cultured Schwann cells secrete platelet-derived growth factor (PDGF) and express PDGF receptors which transmit a mitogenic response particularly when their levels are upregulated by elevation of intracellular cAMP. In this study the expression of PDGF and PDGF alpha- and beta-receptors...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1044
更新日期:1993-02-01 00:00:00
abstract::Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90136-4
更新日期:1988-04-01 00:00:00
abstract::Neonatal mouse hearts fully regenerate after ventricular resection similar to adult zebrafish. We established cryoinjury models to determine if different types and varying degrees of severity in cardiac injuries trigger different responses in neonatal mouse hearts. In contrast to ventricular resection, neonatal mouse ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.12.018
更新日期:2015-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are post-transcriptional regulators of growth and development in both plants and animals. Flowering is critical for the reproduction of angiosperms. Flower development entails the transition from vegetative growth to reproductive growth, floral organ initiation, and the development of floral organs....
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2013.05.009
更新日期:2013-08-15 00:00:00
abstract::The dorsal-ventral axis of the Xenopus embryo is approximately determined by the point of sperm entry, which occurs randomly about the animal-vegetal axis of the egg. Two cytoplasmic components were studied whose spatial arrangements and local concentration patterns appear to reflect the forming dorsal-ventral axis. F...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90457-9
更新日期:1985-06-01 00:00:00
abstract::Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0257
更新日期:1996-10-10 00:00:00
abstract::In the present study, we have investigated the role of tyrosine kinase activity during early retinal development in Xenopus laevis. The protein tyrosine kinase (PTK) inhibitors lavendustin A and genistein were used to determine the possible role of tyrosine kinase activity during retinal development in vivo and in vit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.10.006
更新日期:2004-02-01 00:00:00
abstract::Somatic gonadal niche cells control the survival, differentiation, and proliferation of germline stem cells. The establishment of this niche-stem cell relationship is critical, and yet the precursors to these two cell types are often born at a distance from one another. The simple Caenorhabditis elegans gonadal primor...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.03.019
更新日期:2013-07-15 00:00:00
abstract::The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.04.004
更新日期:2009-06-15 00:00:00
abstract::Specific binding of spermatozoa to the zona pellucida that surrounds mammalian eggs is a key step in the fertilization process. However, the sperm proteins that recognise zona pellucida receptors remain contentious despite longstanding research efforts to identify them. Here we present evidence that proacrosin, a tiss...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.001
更新日期:2007-06-15 00:00:00
abstract::To determine whether spontaneous release of transmitter from the growth cones of neurons exhibits properties similar to the spontaneous release which occurs from the neurons at the neuromuscular junction, release of transmitter from the growth cones of Xenopus neurons in culture was monitored in salines containing var...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90172-7
更新日期:1986-02-01 00:00:00
abstract::Mature hamster eggs exhibit repetitive increases in the intracellular Ca2+ concentration ([Ca2+]i) at fertilization, caused by inositol 1,4,5-trisphosphate (InsP3)-induced Ca2+ release (IICR) from stores. Oscillating Ca2+ rises also occurred in inseminated immature oocytes at the germinal vesicle (GV) stage but the pe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1059
更新日期:1993-03-01 00:00:00
abstract::Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal developmen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90159-5
更新日期:1988-09-01 00:00:00