Abstract：
:Although round spermatid injection can be used to create progeny for males who do not produce mature sperm, the rate of successful embryogenesis after such procedures is significantly lower than that for similar procedures using mature spermatozoa. The mechanisms underlying this difference are unknown. In this study, we demonstrate that, unlike the normal paternal genome, the paternal zygotic genome derived from a round spermatid is highly remethylated before first mitosis after demethylation. Genomes from elongated spermatids exhibited an intermediate level of DNA methylation, between those of round spermatids and mature spermatozoa, suggesting that the male germ cell acquires the ability to maintain its undermethylated state in the paternal zygotic genome during this phase of spermiogenesis. In addition, treatment of zygotes with trichostatin A led to a significant reduction in DNA methylation, specifically in the spermatid-derived paternal genome, except for the pericentromeric regions enriched by trimethylation of Lys9 of histone H3. These data provide insight into epigenetic errors that may be associated with the poor development of embryos generated from immature spermatozoa.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kishigami S,Van Thuan N,Hikichi T,Ohta H,Wakayama S,Mizutani E,Wakayama Tdoi
10.1016/j.ydbio.2005.10.026subject
Has Abstractpub_date
2006-01-01 00:00:00pages
195-205issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(05)00754-2journal_volume
289pub_type
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