Abstract：
:Accumulating evidence indicates that the endometrial extracellular matrix (ECM) modulates trophoblast adhesion during mouse blastocyst implantation. In previous studies of adhesion-competent mouse blastocysts, we have demonstrated that integrin-mediated fibronectin (FN)-binding activity on the apical surface of trophoblast cells is initially low, but becomes strengthened after embryos are exposed to FN. In the present study, we have examined whether the ligand-induced upregulation of trophoblast adhesion to FN is mediated by integrin signaling. The strengthening of adhesion to FN required integrin ligation, which rapidly elevated cytoplasmic-free Ca(2+). Chelation of intracellular Ca(2+) using BAPTA-AM, or inhibition of the Ca(2+)-dependent proteins, protein kinase C or calmodulin, significantly attenuated the effect of FN on binding activity. Furthermore, direct elevation of cytoplasmic Ca(2+) levels with ionomycin upregulated FN-binding activity, demonstrating that Ca(2+) signaling is required and sufficient for strong adhesion to FN. Ca(2+) signaling may induce protein trafficking, a known requirement for ligand-induced upregulation of FN-binding activity. Indeed, intracellular vesicles accumulated in adhesion-competent blastocysts, but were absent after exposure to either FN or ionomycin. These findings suggest that, during implantation, contact between peri-implantation blastocysts and FN elevates intracellular Ca(2+), which strengthens trophoblast adhesion to ECM through protein redistribution.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wang J,Mayernik L,Armant DRdoi
10.1006/dbio.2002.0644subject
Has Abstractpub_date
2002-05-15 00:00:00pages
270-9issue
2eissn
0012-1606issn
1095-564Xpii
S0012160602906445journal_volume
245pub_type
杂志文章abstract：:Using optical imaging assays, we investigated the dynamics of acetylcholine receptors (AChRs) at laminin-associated clusters on cultured myotubes in the absence or presence of the nerve-derived clustering factor, agrin. Using fluorescence recovery after photobleaching (FRAP) on fluorescent bungarotoxin-labeled recepto...
journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex three-dimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development....
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章,评审
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journal_title：Developmental biology
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Developmental biology
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pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1997-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.037
更新日期：2004-05-01 00:00:00
