Abstract：
:Regulation of gene expression during early embryonic development in the rabbit was investigated by quantitative assay of firefly luciferase activity obtained by microinjection of three plasmid constructs using the regulatory region of polyomavirus promoter (PrPyV) with two different enhancer sequences (wild type or mutant "embryo-responsive," ER2) coupled to this reporter gene. Following injection at the 1-cell stage maximal level of expression of these genes was reached after three cell cycles. Two important regulatory steps that progressively limited gene expression were identified: the passage through the first mitosis and the transition from maternal to zygotic control of development (MZT) described at the 8- to 16-cell stage. The completion of the first mitosis was associated with the requirement of an enhancer sequence to stimulate expression of the weak PrPyV promoter while beyond the MZT, only particular enhancer sequences, such as ER2, allowed maintainance of the expression of PrPyV promoter. In addition, comparison of expression of constructs injected in pronuclei, 2-cell embryonic nuclei, and transplanted 32-cell blastomeres revealed that the nuclear environment could be a major effector in the regulation of embryonic gene expression. A schematic view is proposed describing the sequential establishment of the regulation exerted on early embryonic gene expression in progress from the onset of the zygotic genome activity to the MZT.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Christians E,Rao VH,Renard JPdoi
10.1006/dbio.1994.1188subject
Has Abstractpub_date
1994-07-01 00:00:00pages
160-72issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71188-2journal_volume
164pub_type
杂志文章abstract：:Adhesive extracellular matrix (ECM) molecules appear to play roles in the migration of neural crest cells, and may also provide cues for differentiation of these cells into a variety of phenotypes. We are studying the influences of specific ECM components on crest differentiation at the levels of both individual cells...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90112-v
更新日期：1990-09-01 00:00:00
abstract：:For both the intricate morphogenetic layout of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2014.01.023
更新日期：2014-05-01 00:00:00
abstract：:Temporal and spatial aspects of floral determination in seedling terminal buds of the qualitative short-day plant Pharbitis nil were examined using a grafting assay. Floral determination in the terminal buds of 6-day-old P. nil seedlings is rapid; by 9 hr after the end of a 14-hr inductive dark period more than 50% of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90268-n
更新日期：1990-02-01 00:00:00
abstract：:It is well established that the notochord influences the development of adjacent neural and mesodermal tissue. Involvement of the notochord in the differentiation of the dorsal pancreas has been demonstrated. However, our knowledge of the signals involved in pancreatic development is still incomplete. In order to iden...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9348
更新日期：1999-08-15 00:00:00
abstract：:Hox genes encode transcription factors which provide positional information during morphogenesis along the body axis; genetic interaction among Hox genes is thought to be necessary for correct pattern formation. One of the most curious features of the 39 vertebrate Hox genes is that they form four clusters each compos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9651
更新日期：2000-04-15 00:00:00
abstract：:In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with a cis-regulatory target site of the CyIIIa gene. Here we show that this target site, the P7I site, is required for normal e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0050
更新日期：1996-02-25 00:00:00
abstract：:To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9975
更新日期：2001-01-01 00:00:00
abstract：:Metazoan development involves a myriad of dynamic cellular processes that require cytoskeletal function. Nonmuscle myosin II plays essential roles in embryonic development; however, knowledge of its role in post-embryonic development, even in model organisms such as Drosophila melanogaster, is only recently being reve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.06.028
更新日期：2010-09-15 00:00:00
abstract：:Hoxa-11 and Hoxd-11 are paralogous genes required for proper development of the vertebral column, the limbs, and the urogenital system. To further explore the functional relationship between these genes, as well as the potential rescue of one function by the other, we have introduced a Hoxd-11-expressing transgene int...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0137
更新日期：1996-06-15 00:00:00
abstract：:Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90389-1
更新日期：1986-03-01 00:00:00
abstract：:Electroporation-based assays were used to test whether the myogenic regulatory factor (MRF) of Ciona intestinalis (CiMRF) interferes with endogenous developmental programs, and to evaluate the importance of its unusual N-terminus for muscle development. We found that CiMRF suppresses both notochord and endoderm develo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.10.010
更新日期：2019-04-15 00:00:00
abstract：:mid and H15 encode Tbx20 transcription factors that specify ventral pattern in the Drosophila leg. We find that there are at least two pathways for mid and H15 specification of ventral fate. In the first pathway, mid and H15 negatively regulate Dpp, the dorsal signal in leg development. mid and H15 block the dorsalizi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.05.012
更新日期：2019-11-01 00:00:00
abstract：:Copyright ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8954
更新日期：1998-07-15 00:00:00
abstract：:The hox genes play a central role in organogenesis and are implicated in the formation of hematopoietic stem cells (HSCs). The cdx genes encode homeodomain transcription factors that act as master regulators of the hox genes. In zebrafish, mutations in cdx4 cause a severe, but not complete, deficit in embryonic blood ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.003
更新日期：2006-04-15 00:00:00
abstract：:In warm-blooded vertebrates it is generally accepted that after early stages of development new neurons are not added to the retina. Contrary to this belief, we show here that hatched chickens have a zone of proliferating cells at the peripheral margin of the retina, similar to that of fish and amphibians. We found th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9640
更新日期：2000-04-15 00:00:00
abstract：:Hand proteins are evolutionally conserved basic helix-loop-helix (bHLH) transcription factors implicated in development of neural crest-derived tissues, heart and limb. Hand1 is expressed in the distal (ventral) zone of the branchial arches, whereas the Hand2 expression domain extends ventrolaterally to occupy two-thi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.036
更新日期：2007-10-01 00:00:00
abstract：:The fertilization potential in Phallusia mammillata consisted of an initial rapid depolarization. This initial sperm-triggered depolarization was followed by a phase of membrane depolarization which was of either long or short duration, depending on the eggs. When of long duration, the phase of membrane depolarization...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90108-s
更新日期：1992-10-01 00:00:00
abstract：:Polyspermic Xenopus laevis eggs can be identified easily because of regions of pigment accumulation and white stripes, which arise by a nocodazole-sensitive process. Eggs containing up to four sperm are capable of forming a single embryonic axis. Dispermic eggs display two regions of pigment accumulation, one around e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90262-9
更新日期：1986-06-01 00:00:00
abstract：:The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90390-3
更新日期：1987-10-01 00:00:00
abstract：:teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.028
更新日期：2007-07-01 00:00:00
abstract：:Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All thre...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8833
更新日期：1998-03-15 00:00:00
abstract：:The embryonic cell lineage of Caenorhabditis elegans has been traced from zygote to newly hatched larva, with the result that the entire cell lineage of this organism is now known. During embryogenesis 671 cells are generated; in the hermaphrodite 113 of these (in the male 111) undergo programmed death and the remaind...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90201-4
更新日期：1983-11-01 00:00:00
abstract：:Implantation represents a critical step for embryonic development and pregnancy. Its success depends on the complex interplay between a receptive endometrium and a competent embryo. Implantation-related events remain hardly accessible, making implantation a true "black box" in developmental biology. Improved in vitro ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2020.04.003
更新日期：2020-07-01 00:00:00
abstract：:BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1Delt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.036
更新日期：2010-03-01 00:00:00
abstract：:The location and movement of mammalian gut tissue progenitors, prior to the expression of tissue-specific genes, has been unknown, but this knowledge is essential to identify transitions that lead to cell type specification. To address this, we used vital dyes to label exposed anterior endoderm cells of early somite s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.01.003
更新日期：2005-04-01 00:00:00
abstract：:Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.008
更新日期：2005-06-01 00:00:00
abstract：:The subventricular zone (SVZ) of the developing mammalian forebrain gives rise to astrocytes and oligodendrocytes in the neocortex and white matter, and neurons in the olfactory bulb in perinatal life. We have examined the developmental fates and spatial distributions of the descendants of single SVZ cells by infectin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.02.024
更新日期：2004-06-01 00:00:00
abstract：:Pericellular proteolysis by ADAM family metalloproteinases has been widely implicated in cell signaling and development. We recently found that Xenopus ADAM13, an ADAM metalloproteinase, is required for activation of canonical Wnt signaling during cranial neural crest (CNC) induction by regulating a novel crosstalk be...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.12.031
更新日期：2012-03-01 00:00:00
abstract：:Understanding the regulatory circuitry controlling myogenesis is critical to understanding developmental mechanisms and developmentally-derived diseases. We analyzed the transcriptional regulation of a Drosophila myogenic repressor gene, Holes in muscles (Him). Previously, Him was shown to inhibit Myocyte enhancer fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.02.005
更新日期：2015-04-15 00:00:00
