In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development.


:Distal-less 3 (DLX3) gene mutations are etiologic for Tricho-Dento-Osseous syndrome. To investigate the in vivo impact of mutant DLX3 on bone development, we established transgenic (TG) mice expressing the c.571_574delGGGG DLX-3 gene mutation (MT-DLX3) driven by a mouse 2.3 Col1A1 promoter. Microcomputed tomographic analyses demonstrated markedly increased trabecular bone volume and bone mineral density in femora from TG mice. In ex vivo experiments, TG mice showed enhanced differentiation of bone marrow stromal cells to osteoblasts and increased expression levels of bone formation markers. However, TG mice did not show enhanced dynamic bone formation rates in in vivo fluorochrome double labeling experiments. Osteoclastic differentiation capacities of bone marrow monocytes were reduced in TG mice in the presence of osteoclastogenic factors and the numbers of TRAP(+) osteoclasts on distal metaphyseal trabecular bone surfaces were significantly decreased. TRACP 5b and CTX serum levels were significantly decreased in TG mice, while IFN-gamma levels were significantly increased. These data demonstrate that increased levels of IFN-gamma decrease osteoclast bone resorption activities, contributing to the enhanced trabecular bone volume and mineral density in these TG mice. These data suggest a novel role for this DLX-3 mutation in osteoclast differentiation and bone resorption.


Dev Biol


Developmental biology


Choi SJ,Roodman GD,Feng JQ,Song IS,Amin K,Hart PS,Wright JT,Haruyama N,Hart TC




Has Abstract


2009-01-01 00:00:00














  • Gene regulation by the lysine demethylase KDM4A in Drosophila.

    abstract::Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle- or developmental stage. KDM4A demeth...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Crona F,Dahlberg O,Lundberg LE,Larsson J,Mannervik M

    更新日期:2013-01-15 00:00:00

  • Myf5 and MyoD activation define independent myogenic compartments during embryonic development.

    abstract::Gene targeting has indicated that Myf5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are missing in mouse embryos lacking both Myf5 and MyoD. To investigate the fate of Myf5:MyoD-deficient myogenic precursor cells during embryogenesis, we examined the sites of epaxial, hypax...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kablar B,Krastel K,Tajbakhsh S,Rudnicki MA

    更新日期:2003-06-15 00:00:00

  • Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila.

    abstract::The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Inoue Y,Hayashi S

    更新日期:2007-04-01 00:00:00

  • Chromatin associated Sin3A is essential for male germ cell lineage in the mouse.

    abstract::Spermatogenesis is a complex process that requires coordinated proliferation and differentiation of male germ cells. The molecular events that dictate this process are largely unknown, but are likely to involve highly regulated transcriptional control. In this study, we investigate the contribution of chromatin associ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Pellegrino J,Castrillon DH,David G

    更新日期:2012-09-15 00:00:00

  • Choroideremia gene product affects trophoblast development and vascularization in mouse extra-embryonic tissues.

    abstract::Choroideremia (CHM) is a hereditary eye disease caused by mutations in the X-linked CHM gene. Disruption of the Chm gene in mice resulted in prenatal death of Chm-/Y males and Chm-/Chm+ females that had inherited the mutation from their mothers. Male chimeras and Chm+/Chm- females with paternal transmission of the mut...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shi W,van den Hurk JA,Alamo-Bethencourt V,Mayer W,Winkens HJ,Ropers HH,Cremers FP,Fundele R

    更新日期:2004-08-01 00:00:00

  • Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis.

    abstract::Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Whitworth GB,Misaghi BC,Rosenthal DM,Mills EA,Heinen DJ,Watson AH,Ives CW,Ali SH,Bezold K,Marsh-Armstrong N,Watson FL

    更新日期:2017-06-15 00:00:00

  • Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII) regulates growth and patterning of the postnatal mouse cerebellum.

    abstract::COUP-TFII (also known as Nr2f2), a member of the nuclear orphan receptor superfamily, is expressed in several regions of the central nervous system (CNS), including the ventral thalamus, hypothalamus, midbrain, pons, and spinal cord. To address the function of COUP-TFII in the CNS, we generated conditional COUP-TFII k...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kim BJ,Takamoto N,Yan J,Tsai SY,Tsai MJ

    更新日期:2009-02-15 00:00:00

  • A glycoprotein from the liver constitutes the inner layer of the egg envelope (zona pellucida interna) of the fish, Oryzias latipes.

    abstract::A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Hamazaki TS,Nagahama Y,Iuchi I,Yamagami K

    更新日期:1989-05-01 00:00:00

  • Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression.

    abstract::Rex1 (Zfp42), GeneID 132625, is a gene whose expression is closely associated with pluripotency/multipotency in both mouse and human embryonic stem cells. To study the function of the murine Rex1 gene in vivo, we have used cre/lox technology to create Rex1(floxed) mice and mice deficient in Rex1 gene function. Rex1(-/...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rezende NC,Lee MY,Monette S,Mark W,Lu A,Gudas LJ

    更新日期:2011-08-15 00:00:00

  • Inhibition of protein kinases after an induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion.

    abstract::We have examined the response of bovine oocytes matured in vitro for 24 hr to parthenogenic activation using compounds that increase intracellular calcium (ionomycin) or inhibit protein phosphorylation (6-dimethylaminopurine, DMAP). Treatment with ionomycin alone caused resumption of meiosis (57.8 +/- 7.8%) but not pr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Susko-Parrish JL,Leibfried-Rutledge ML,Northey DL,Schutzkus V,First NL

    更新日期:1994-12-01 00:00:00

  • The Pit-1 gene is regulated by distinct early and late pituitary-specific enhancers.

    abstract::The differentiation of three anterior pituitary cell types is regulated by the tissue-specific POU domain factor Pit-1, which is initially expressed on Embryonic Day 13.5-14 in mice. The Pit-1 gene remains continuously, highly expressed in the somatotrope, thyrotrope, and lactotrope cells of the adult. Using the Pit-1...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: DiMattia GE,Rhodes SJ,Krones A,Carrière C,O'Connell S,Kalla K,Arias C,Sawchenko P,Rosenfeld MG

    更新日期:1997-02-01 00:00:00

  • Cell-cell interactions trigger the rapid induction of a specific high mobility group-like protein during early stages of conjugation in Tetrahymena.

    abstract::Conjugation in Tetrahymena represents an ordered developmental pathway which represents the sexual phase of the ciliate life cycle. This pathway is initiated when starved cells of opposite mating types are mixed and are allowed to make a series of cell-cell contacts (a period termed costimulation) which lead to the fo...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Schulman IG,Wang TT,Stargell LA,Gorovsky MA,Allis CD

    更新日期:1991-02-01 00:00:00

  • The implication of microRNAs and endo-siRNAs in animal germline and early development.

    abstract::Germ cells provide maternal mRNAs that are stored in the oocyte, and later translated at a specific time of development. In this context, gene regulation depends mainly on post-transcriptional mechanisms that contribute to keep maternal transcripts in a stable and translationally silent state. In recent years, small n...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Dallaire A,Simard MJ

    更新日期:2016-08-01 00:00:00

  • Mammalian target of rapamycin complex 1 (mTORC1) Is required for mouse spermatogonial differentiation in vivo.

    abstract::Spermatogonial stem cells (SSCs) must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. This self-renewal vs. differentiation spermatogonial fate decision is critical for maintaining tissue homeostasis, as imbalances cau...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Busada JT,Niedenberger BA,Velte EK,Keiper BD,Geyer CB

    更新日期:2015-11-01 00:00:00

  • Retinoic acid signaling is dispensable for somatic development and function in the mammalian ovary.

    abstract::Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiati...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Minkina A,Lindeman RE,Gearhart MD,Chassot AA,Chaboissier MC,Ghyselinck NB,Bardwell VJ,Zarkower D

    更新日期:2017-04-15 00:00:00

  • Periodic movements of Dictyostelium discoideum sorocarps.

    abstract::We report periodic movements during erection of Dictyostelium discoideum (Dd) sorocarps. Our observations lead to the working hypothesis that Dd sorocarp erection occurs by two superimposed processes: one periodic, with a modal period of 6 1/2 min, and one continuous. We tentatively identify the periodic process with ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Durston AJ,Cohen MH,Drage DJ,Potel MJ,Robertson A,Wonio D

    更新日期:1976-09-01 00:00:00

  • The role of GDNF in patterning the excretory system.

    abstract::Mesenchymal-epithelial interactions are an important source of information for pattern formation during organogenesis. In the developing excretory system, one of the secreted mesenchymal factors thought to play a critical role in patterning the growth and branching of the epithelial ureteric bud is GDNF. We have teste...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shakya R,Jho EH,Kotka P,Wu Z,Kholodilov N,Burke R,D'Agati V,Costantini F

    更新日期:2005-07-01 00:00:00

  • The timing of expression of four actin genes and an RNA polymerase III-transcribed repeated sequence is correct in hybrid embryos of the sea urchin species Strongylocentrotus purpuratus and Lytechinus pictus.

    abstract::The accumulation of the mRNAs from four Strongylocentrotus purpuratus actin genes (the single muscle gene M, and three cytoskeletal genes CyI, CyIIIa, and CyIIIb) and of transcripts from an RNA polymerase III-transcribed repeated sequence family (SURF1) was followed throughout the early development of hybrid embryos o...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bullock BP,Nisson PE,Crain WR Jr

    更新日期:1988-11-01 00:00:00

  • Reporter gene expression in G2 of the 1-cell mouse embryo.

    abstract::Similar amounts of expression of a luciferase reporter gene that is driven by the SV40 early promoter are detected in G2 of 1-cell mouse embryo following injection of the male pronucleus in either early or mid/late S. No luciferase activity is observed when the male pronucleus is injected in early S and the embryos an...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ram PT,Schultz RM

    更新日期:1993-04-01 00:00:00

  • Size-dependent regulation of dorsal-ventral patterning in the early Drosophila embryo.

    abstract::How natural variation in embryo size affects patterning of the Drosophila embryo dorsal-ventral (DV) axis is not known. Here we examined quantitatively the relationship between nuclear distribution of the Dorsal transcription factor, boundary positions for several target genes, and DV axis length. Data were obtained f...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Garcia M,Nahmad M,Reeves GT,Stathopoulos A

    更新日期:2013-09-01 00:00:00

  • The direction of neurite growth in a weak DC electric field depends on the substratum: contributions of adhesivity and net surface charge.

    abstract::We investigated the influence of the growth surface on the direction of Xenopus spinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue cul...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rajnicek AM,Robinson KR,McCaig CD

    更新日期:1998-11-15 00:00:00

  • An organizing role for the TGF-β signaling pathway in axes formation of the annelid Capitella teleta.

    abstract::Embryonic organizers are signaling centers that coordinate developmental events within an embryo. Localized to either an individual cell or group of cells, embryonic organizing activity induces the specification of other cells in the embryo and can influence formation of body axes. In the spiralian Capitella teleta, p...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lanza AR,Seaver EC

    更新日期:2018-03-01 00:00:00

  • CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition.

    abstract::The sub-division of the posterior-most territory of the neural plate results in the formation of two distinct neural structures, the hindbrain and the spinal cord. Although many of the molecular signals regulating the development of these individual structures have been elucidated, the mechanisms involved in delineati...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Chang J,Skromne I,Ho RK

    更新日期:2016-02-15 00:00:00

  • Translational regulation of the novel haploid-specific transcripts for the c-abl proto-oncogene and a member of the 70 kDa heat-shock protein gene family in the male germ line.

    abstract::Expression of the c-abl proto-oncogene in the mouse testis is characterized by the production of a unique 4.7-kb transcript present in germ cells that have entered the haploid phase of spermatogenesis. A similar developmental stage specificity of expression is observed for a member of the 70-kDa heat-shock protein (hs...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zakeri ZF,Ponzetto C,Wolgemuth DJ

    更新日期:1988-02-01 00:00:00

  • Functional analysis of Cdc42 in actin filament assembly, epithelial morphogenesis, and cell signaling during Drosophila development.

    abstract::Cdc42, a member of the Rho family of GTP binding proteins, functions in the formation of polarized actin structures, in elongation of cell shape, and in cell signaling. Although genetic mutations previously have not been available in multicellular organisms, studies have attempted to discern Cdc42 functions in organis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Genova JL,Jong S,Camp JT,Fehon RG

    更新日期:2000-05-01 00:00:00

  • Defective adult oligodendrocyte and Schwann cell development, pigment pattern, and craniofacial morphology in puma mutant zebrafish having an alpha tubulin mutation.

    abstract::The processes of myelination remain incompletely understood but are of profound biomedical importance owing to the several dysmyelinating and demyelinating disorders known in humans. Here, we analyze the zebrafish puma mutant, isolated originally for pigment pattern defects limited to the adult stage. We show that pum...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Larson TA,Gordon TN,Lau HE,Parichy DM

    更新日期:2010-10-15 00:00:00

  • The dorsal spinal cord and hindbrain: From developmental mechanisms to functional circuits.

    abstract::Neurons of the dorsal hindbrain and spinal cord are central in receiving, processing and relaying sensory perception and participate in the coordination of sensory-motor output. Numerous cellular and molecular mechanisms that underlie neuronal development in both regions of the nervous system are shared. We discuss he...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Hernandez-Miranda LR,Müller T,Birchmeier C

    更新日期:2017-12-01 00:00:00

  • Characterization of ADP-ribosyl cyclase 1-like (ARC1-like) activity and NAADP signaling during slow muscle cell development in zebrafish embryos.

    abstract::We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger(s) of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kelu JJ,Webb SE,Galione A,Miller AL

    更新日期:2019-01-15 00:00:00

  • Effect of hydrocortisone on skin development in the chick embryo: ultrastructural, immunohistological, and biochemical analysis.

    abstract::The effect of hydrocortisone on the development of dorsal skin was analyzed in the chick embryo by (1) transmission electron microscopy, (2) indirect immunofluorescence histology of extracellular matrix components (collagen types I, III, and IV; fibronectin; and laminin), and (3) quantitative determination of collagen...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Démarchez M,Mauger A,Herbage D,Sengel P

    更新日期:1984-11-01 00:00:00

  • The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity.

    abstract::Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Heller RS,Stoffers DA,Liu A,Schedl A,Crenshaw EB 3rd,Madsen OD,Serup P

    更新日期:2004-04-01 00:00:00