当前位置： SCI文献检索 > DEVELOPMENTAL BIOLOGY期刊下所有文献 > GRAF1 promotes ferlin-dependent myoblast fusion.

GRAF1 promotes ferlin-dependent myoblast fusion.

Abstract：

:Myoblast fusion (a critical process by which muscles grow) occurs in a multi-step fashion that requires actin and membrane remodeling; but important questions remain regarding the spatial/temporal regulation of and interrelationship between these processes. We recently reported that the Rho-GAP, GRAF1, was particularly abundant in muscles undergoing fusion to form multinucleated fibers and that enforced expression of GRAF1 in cultured myoblasts induced robust fusion by a process that required GAP-dependent actin remodeling and BAR domain-dependent membrane sculpting. Herein we developed a novel line of GRAF1-deficient mice to explore a role for this protein in the formation/maturation of myotubes in vivo. Post-natal muscles from GRAF1-depleted mice exhibited a significant and persistent reduction in cross-sectional area, impaired regenerative capacity and a significant decrease in force production indicative of lack of efficient myoblast fusion. A significant fusion defect was recapitulated in isolated myoblasts depleted of GRAF1 or its closely related family member GRAF2. Mechanistically, we show that GRAF1 and 2 facilitate myoblast fusion, at least in part, by promoting vesicle-mediated translocation of fusogenic ferlin proteins to the plasma membrane.

journal_name

Dev Biol

journal_title

Developmental biology

authors

Lenhart KC,Becherer AL,Li J,Xiao X,McNally EM,Mack CP,Taylor JM

doi

10.1016/j.ydbio.2014.06.025

subject

Has Abstract

pub_date

2014-09-15 00:00:00

pages

298-311

issue

2

eissn

0012-1606

issn

1095-564X

pii

S0012-1606(14)00321-2

journal_volume

393

pub_type

杂志文章

相关文献

DEVELOPMENTAL BIOLOGY文献大全
  • The NK homeodomain transcription factor Tinman is a direct activator of seven-up in the Drosophila dorsal vessel.

    abstract：:A complex regulatory cascade is required for normal cardiac development, and many aspects of this network are conserved from Drosophila to mammals. In Drosophila, the seven-up (svp) gene, an ortholog of the vertebrate chick ovalbumin upstream promoter transcription factors (COUP-TFI and II), is initially activated in ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2006.10.025

    authors： Ryan KM,Hendren JD,Helander LA,Cripps RM

    更新日期：2007-02-15 00:00:00

  • The fub-1 mutation blocks initial myofibril formation in zebrafish muscle pioneer cells.

    abstract：:The earliest muscle in zebrafish arises from iterated sets of two to six cells in each somite, the muscle pioneers (MP). MP develop synchronously in young trunk myotomes adjacent to the notochord, precisely where the horizontal myoseptum will form. They elongate without cell fusion and differentiate hours earlier than...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(91)90314-s

    authors： Felsenfeld AL,Curry M,Kimmel CB

    更新日期：1991-11-01 00:00:00

  • Direct interaction between Teashirt and Sex combs reduced proteins, via Tsh's acidic domain, is essential for specifying the identity of the prothorax in Drosophila.

    abstract：:teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2007.04.028

    authors： Taghli-Lamallem O,Gallet A,Leroy F,Malapert P,Vola C,Kerridge S,Fasano L

    更新日期：2007-07-01 00:00:00

  • The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.

    abstract：:Immature, Stage VI oocytes of Xenopus laevis fail to activate (i.e., to propagate a cortical reaction and elevate a fertilization envelope) when pricked or exposed to A23187. We determined the times during maturation when immature oocytes treated with progesterone in vitro developed the capacity to respond to pricking...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(84)90177-5

    authors： Charbonneau M,Grey RD

    更新日期：1984-03-01 00:00:00

  • Syd/JIP3 controls tissue size by regulating Diap1 protein turnover downstream of Yorkie/YAP.

    abstract：:How organisms control organ size is not fully understood. We found that Syd/JIP3 is required for proper wing size in Drosophila. JIP3 mutations are associated with organ size defects in mammals. The underlying mechanisms are not well understood. We discovered that Syd/JIP3 inhibition results in a downregulation of the...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2020.09.017

    authors： Ahmad V,Vadla GP,Chabu CY

    更新日期：2021-01-01 00:00:00

  • Perturbation of cranial neural crest migration by the HNK-1 antibody.

    abstract：:The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(87)90390-3

    authors： Bronner-Fraser M

    更新日期：1987-10-01 00:00:00

  • cMeso-1, a novel bHLH transcription factor, is involved in somite formation in chicken embryos.

    abstract：:The segmentation of somites from the paraxial mesoderm is a crucial event in vertebrate embryonic development; however, the mechanisms underlying this process are not well understood. In a yeast two-hybrid screen we have identified the novel basic-helix-loop-helix (bHLH) protein cMeso-1 which is expressed in the preso...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1998.8919

    authors： Buchberger A,Seidl K,Klein C,Eberhardt H,Arnold HH

    更新日期：1998-07-15 00:00:00

  • Slowing of synapse elimination by alpha-bungarotoxin superfusion of the neonatal rabbit soleus muscle.

    abstract：:To examine the role of postsynaptic activity in regulating the rate of neuromuscular synapse elimination, contractile activity of neonatal rabbit soleus muscles was decreased by chronic superfusion of alpha-bungarotoxin (alpha-BGT) over their surfaces. Superfusion was begun at 6 days postnatal and continued for a vari...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/s0012-1606(89)80009-0

    authors： Callaway EM,Van Essen DC

    更新日期：1989-02-01 00:00:00

  • Apoptosis in the neuronal lineage of the mouse olfactory epithelium: regulation in vivo and in vitro.

    abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1995.0025

    authors： Holcomb JD,Mumm JS,Calof AL

    更新日期：1995-11-01 00:00:00

  • PITX1 promotes chondrogenesis and myogenesis in mouse hindlimbs through conserved regulatory targets.

    abstract：:The PITX1 transcription factor is expressed during hindlimb development, where it plays a critical role in directing hindlimb growth and the specification of hindlimb morphology. While it is known that PITX1 regulates hindlimb formation, in part, through activation of the Tbx4 gene, other transcriptional targets remai...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2017.12.013

    authors： Wang JS,Infante CR,Park S,Menke DB

    更新日期：2018-02-01 00:00:00

  • Analysis of human embryos from zygote to blastocyst reveals distinct gene expression patterns relative to the mouse.

    abstract：:Early mammalian embryogenesis is controlled by mechanisms governing the balance between pluripotency and differentiation. The expression of early lineage-specific genes can vary significantly between species, with implications for developmental control and stem cell derivation. However, the mechanisms involved in patt...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2012.12.008

    authors： Niakan KK,Eggan K

    更新日期：2013-03-01 00:00:00

  • A screen for downstream effectors of Neurogenin2 in the embryonic neocortex.

    abstract：:Neurogenin (Ngn) 1 and Ngn2 encode basic-helix-loop-helix transcription factors expressed in the developing neocortex. Like other proneural genes, Ngns participate in the specification of neural fates and neuronal identities, but downstream effectors remain poorly defined. We set out to identify Ngn2 effectors in the ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2004.06.013

    authors： Mattar P,Britz O,Johannes C,Nieto M,Ma L,Rebeyka A,Klenin N,Polleux F,Guillemot F,Schuurmans C

    更新日期：2004-09-15 00:00:00

  • DNA sequences mediating the transcriptional response of the Mix.2 homeobox gene to mesoderm induction.

    abstract：:Peptide growth factors can initiate changes in cell fate in Xenopus ectodermal explants and induce the formation of mesoderm. Marker genes expressed in mesoderm allow the analysis of whether, or how much, induction has occurred, but do not tell us what molecules are involved in carrying out the response. In this repor...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1996.0158

    authors： Vize PD

    更新日期：1996-07-10 00:00:00

  • An overlapping CArG/octamer element is required for regulation of desmin gene transcription in arterial smooth muscle cells.

    abstract：:The desmin gene encodes an intermediate filament protein that is present in skeletal, cardiac, and smooth muscle cells. This study shows that the 4-kb upstream region of the murine desmin promoter directs expression of a lacZ reporter gene throughout the heart from E7.5 and in skeletal muscle and vascular smooth muscl...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.2000.9865

    authors： Mericskay M,Parlakian A,Porteu A,Dandré F,Bonnet J,Paulin D,Li Z

    更新日期：2000-10-15 00:00:00

  • Gene regulation by the lysine demethylase KDM4A in Drosophila.

    abstract：:Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle- or developmental stage. KDM4A demeth...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2012.11.011

    authors： Crona F,Dahlberg O,Lundberg LE,Larsson J,Mannervik M

    更新日期：2013-01-15 00:00:00

  • Involvement of Rho family G protein in the cell signaling for sperm incorporation during fertilization of mouse eggs: inhibition by Clostridium difficile toxin B.

    abstract：:Sperm-egg interaction was investigated in mouse eggs freed from the zona pellucida and injected with Clostridium difficile toxin B, the inhibitor of Rho family small G proteins. Toxin B reduced in a dose-dependent manner the percentage of eggs associated with sperm fusion on the surface or sperm nucleus decondensation...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/s0012-1606(03)00273-2

    authors： Kumakiri J,Oda S,Kinoshita K,Miyazaki S

    更新日期：2003-08-15 00:00:00

  • Growing dictyate oocytes, but not early preimplantation embryos, of the mouse display high levels of DNA homologous recombination by single-strand annealing and lack DNA nonhomologous end joining.

    abstract：:We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.2001.0199

    authors： Fiorenza MT,Bevilacqua A,Bevilacqua S,Mangia F

    更新日期：2001-05-01 00:00:00

  • Foxe3 is required for morphogenesis and differentiation of the anterior segment of the eye and is sensitive to Pax6 gene dosage.

    abstract：:The dysgenetic lens (dyl) mouse mutant has mutations in Foxe3, which inactivate DNA binding by the encoded forkhead transcription factor. Here we confirm, by targeted inactivation, that Foxe3 mutations are responsible for the dyl phenotype, which include loss of lens epithelium; a small, cataractic lens; and failure o...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2006.09.021

    authors： Blixt A,Landgren H,Johansson BR,Carlsson P

    更新日期：2007-02-01 00:00:00

  • Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells.

    abstract：:P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and c...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(90)90202-t

    authors： Rudnicki MA,Jackowski G,Saggin L,McBurney MW

    更新日期：1990-04-01 00:00:00

  • Elk3 is essential for the progression from progenitor to definitive neural crest cell.

    abstract：:Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we chara...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2012.12.009

    authors： Rogers CD,Phillips JL,Bronner ME

    更新日期：2013-02-15 00:00:00

  • An N-linked carbohydrate-containing extracellular matrix determinant plays a key role in sea urchin gastrulation.

    abstract：:During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1994.1154

    authors： Ingersoll EP,Ettensohn CA

    更新日期：1994-06-01 00:00:00

  • Identification of fetomodulin, a surface marker protein of fetal development, as thrombomodulin by gene cloning and functional assays.

    abstract：:Fetomodulin (FM) was previously shown to be a surface marker protein of parietal endoderm by in vitro differentiation of F9 embryonal carcinoma cells and by immunohistochemistry of in vivo embryos. BALB/3T3 and sarcoma S180 cells of the mouse were also shown to possess a protein which was indistinguishable from FM by ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(90)90058-q

    authors： Imada S,Yamaguchi H,Nagumo M,Katayanagi S,Iwasaki H,Imada M

    更新日期：1990-07-01 00:00:00

  • Cul4A is essential for spermatogenesis and male fertility.

    abstract：:The mammalian Cul4 genes, Cul4A and Cul4B, encode the scaffold components of the cullin-based E3 ubiquitin ligases. The two Cul4 genes are functionally redundant. Recent study indicated that mice expressing a truncated CUL4A that fails to interact with its functional partner ROC1 exhibit no developmental phenotype. We...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2011.01.028

    authors： Kopanja D,Roy N,Stoyanova T,Hess RA,Bagchi S,Raychaudhuri P

    更新日期：2011-04-15 00:00:00

  • Cell migration: Rho GTPases lead the way.

    abstract：:Rho GTPases control signal transduction pathways that link cell surface receptors to a variety of intracellular responses. They are best known as regulators of the actin cytoskeleton, but in addition they control cell polarity, gene expression, microtubule dynamics and vesicular trafficking. Through these diverse func...

    journal_title：Developmental biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ydbio.2003.06.003

    authors： Raftopoulou M,Hall A

    更新日期：2004-01-01 00:00:00

  • AMPK regulation of mouse oocyte meiotic resumption in vitro.

    abstract：:We have previously shown that the adenosine analog 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR), an activator of AMP-activated protein kinase (AMPK), stimulates an increase in AMPK activity and induces meiotic resumption in mouse oocytes [Downs, S.M., Hudson, E.R., Hardie, D.G., 2002. A potential rol...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2005.11.039

    authors： Chen J,Hudson E,Chi MM,Chang AS,Moley KH,Hardie DG,Downs SM

    更新日期：2006-03-15 00:00:00

  • Equarin is involved as an FGF signaling modulator in chick lens differentiation.

    abstract：:Lens growth involves the proliferation of epithelial cells, followed by their migration to the equator region and differentiation into secondary fiber cells. It is widely accepted that fibroblast growth factor (FGF) signaling is required for the differentiation of lens epithelial cells into crystallin-rich fibers, but...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2012.05.029

    authors： Song X,Sato Y,Felemban A,Ito A,Hossain M,Ochiai H,Yamamoto T,Sekiguchi K,Tanaka H,Ohta K

    更新日期：2012-08-01 00:00:00

  • Cell death of motoneurons in the chick embryo spinal cord. VIII. Motoneurons prevented from dying in the embryo persist after hatching.

    abstract：:A chronic neuromuscular blockade during those embryonic stages when naturally occurring spinal motoneuron death occurs, results in the prevention of this cell loss. The excess motoneurons are maintained as long as the neuromuscular blockade is continued; once embryonic neuromuscular activity resumes, however, the exce...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(84)90114-3

    authors： Oppenheim RW

    更新日期：1984-01-01 00:00:00

  • Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish.

    abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2008.04.042

    authors： Emelyanov A,Parinov S

    更新日期：2008-08-01 00:00:00

  • Competition for factors and cellular resources as a principle of pattern formation in Hydra. II. Assistance of foot formation by heads and buds and a new model of pattern control.

    abstract：:In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1995.1015

    authors： Müller WA

    更新日期：1995-01-01 00:00:00

  • SKR-1, a homolog of Skp1 and a member of the SCF(SEL-10) complex, regulates sex-determination and LIN-12/Notch signaling in C. elegans.

    abstract：:Sex-determination in Caenorhabditis elegans requires regulation of gene transcription and protein activity and stability. sel-10 encodes a WD40-repeat-containing F-box protein that likely mediates the ubiquitin-mediated degradation of important sex-determination factors. Loss of sel-10 results in a mild masculinizatio...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2008.07.035

    authors： Killian DJ,Harvey E,Johnson P,Otori M,Mitani S,Xue D

    更新日期：2008-10-15 00:00:00