Abstract:
:To examine the role of postsynaptic activity in regulating the rate of neuromuscular synapse elimination, contractile activity of neonatal rabbit soleus muscles was decreased by chronic superfusion of alpha-bungarotoxin (alpha-BGT) over their surfaces. Superfusion was begun at 6 days postnatal and continued for a variable duration (2 to 5 days) before muscles were analyzed. The percentage of polyinnervated fibers was assessed both physiologically and anatomically for alpha-BGT-treated muscles and their contralateral muscles, in addition to normal and control muscles of the same age. Within muscles exposed to alpha-BGT, polyinnervation was significantly greater than that for muscles from each of the control groups. The anatomical assay further revealed that the retention of polyinnervation in alpha-BGT-treated muscles was most pronounced near the muscle's surface, although end plates at the center were also affected. This finding, coupled with evidence that only a small percentage of the muscle fibers were completely inactivated, suggests that the activity block was also most pronounced near the surface and relatively low at the muscle's center. The percentage of end plates at which synapse elimination was delayed was greater than the estimated percentage whose activity was completely blocked, suggesting that synapse loss was slowed even in muscle fibers retaining some postsynaptic activity. These observations indicate that the rate of synapse elimination depends on the levels of functional acetylcholine receptors. This process could be mediated in a graded fashion by changes in postsynaptic activity (subthreshold or suprathreshold) or by a nonelectrical effect of blocking postsynaptic receptors.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Callaway EM,Van Essen DCdoi
10.1016/s0012-1606(89)80009-0subject
Has Abstractpub_date
1989-02-01 00:00:00pages
356-65issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(89)80009-0journal_volume
131pub_type
杂志文章abstract::Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.009
更新日期:2011-01-15 00:00:00
abstract::The embryonic chick face is composed of a series of facial primordia, epithelium-covered buds of mesenchyme, which surround the presumptive mouth. The protruding adult upper beak containing the prenasal cartilage is formed from the frontonasal mass, the paired maxillary primordia form the sides of the face, while the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90142-5
更新日期:1989-11-01 00:00:00
abstract::The development of the Drosophila leg is a good model to study processes of pattern formation, cell death and segmentation. Such processes require the coordinate activity of different genes and signaling pathways that progressively subdivide the leg territory into smaller domains. One of the main pathways needed for l...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2014-01-15 00:00:00
abstract::The myelin of the peripheral nervous system from the shiverer mutant mice is characterized by the absence of myelin basic protein, while the other myelin protein components are present at normal levels. Myelin lamella formation is normal in the shiverer mutant. Therefore, by using antiserum against myelin basic protei...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90277-x
更新日期:1984-09-01 00:00:00
abstract::Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.014
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.011
更新日期:2009-11-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.010
更新日期:2003-11-15 00:00:00
abstract::The four members of the albumin gene family encode the serum transport proteins albumin, alpha-fetoprotein, alpha-albumin, and vitamin D-binding protein. These genes are transcribed primarily in the liver with each having a different pattern of developmental expression. The tight linkage of these genes, particularly t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.026
更新日期:2009-12-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90084-v
更新日期:1990-08-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.04.015
更新日期:2011-07-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1301
更新日期:1993-11-01 00:00:00
abstract::The molting cycle in insects is regulated by the fluctuating levels of ecdysteroids. Twenty-four hours prior to the pupal molt in Manduca sexta steroid titers are relatively high and they fall to very low levels at 4 hr before the molt. Several physiological events accompanying molts have been shown to require the ris...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1115
更新日期:1994-04-01 00:00:00
abstract::The engrailed gene is well known from its role in segmentation and central nervous system development in a variety of species. In molluscs, however, engrailed is involved in shell formation. So far, it seemed that engrailed had been co-opted uniquely for this particular process in molluscs. Here, we show that, in the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0653
更新日期:2002-06-15 00:00:00
abstract::The ubiquitin-proteasome system is essential for intracellular protein degradation, but there are few studies of this system in the extracellular milieu. Recently, we reported that a 70-kDa sperm receptor, HrVC70, on the vitelline coat is ubiquitinated and then degraded by the sperm proteasome during fertilization of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.016
更新日期:2003-12-01 00:00:00
abstract::During early vertebrate development, members of the transforming growth factor beta (TGFbeta) family play important roles in a variety of processes, including germ layer specification, patterning, cell differentiation, migration, and organogenesis. The activities of TGFbetas need to be tightly controlled to ensure the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00075-1
更新日期:2003-03-01 00:00:00
abstract::In higher organisms, mononucleated myoblasts fuse to form multinucleated myotubes. During this process, myoblasts undergo specific changes in cell morphology and cytoarchitecture. Previously, we have shown that the actin regulator Kette (Hem-2/Nap-1) is essential for myoblast fusion. In this study, we describe the rol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.015
更新日期:2007-04-15 00:00:00
abstract::Zygotic Wnt signaling has been shown to be involved in dorsoventral mesodermal patterning in Xenopus embryos, but how it regulates different myogenic gene expression in the lateral mesodermal domains is not clear. Here, we use transient exposure of embryos or explants to lithium, which mimics Wnt/beta-catenin signalin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0633
更新日期:2002-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.028
更新日期:2007-07-01 00:00:00
abstract::To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.007
更新日期:2007-02-01 00:00:00
abstract::Mammalian sperm must acquire their fertilizing ability after a series of biochemical modifications in the female reproductive tract collectively called capacitation to undergo acrosomal exocytosis, a process that is essential for fertilization. Actin dynamics play a central role in controlling the process of exocytosi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.008
更新日期:2015-09-15 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1999-07-15 00:00:00
abstract::Three unrelated chromosome 2q14.1-14.2 region translocations caused the split hand/foot limb malformation development in humans by an unknown mechanism. Their etiology was described by the autosomal dominant inheritance with incomplete penetrance genetic model although authors stated, "the understanding of the genotyp...
journal_title:Developmental biology
pub_type: 杂志文章,评审
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更新日期:2015-12-01 00:00:00
abstract::To gain an understanding of the genes and mechanisms that govern morphogenesis and its evolution, we have analyzed mutations that disrupt this process in a simple model structure, the male tail tip of the rhabditid nematode C. elegans. During the evolution of rhabditid male tails, there have been several independent c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.472
更新日期:2006-09-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9847
更新日期:2000-10-01 00:00:00
abstract::Intercellular communication mediated by gap junctions is important for tissue homeostasis in the avascular lens, and extensive areas of gap junctions form between fiber cells during fiber cell differentiation and lens development. We examined the role of the calcium-dependent cell adhesion molecule, N-cadherin, in the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0237
更新日期:1996-10-10 00:00:00
abstract::XX gonads differentiate into ovaries, a morphologic event evident by embryonic day 13.5 (E13.5) in mice. To identify early markers of oogenesis, sex-specific urogenital ridge cDNA libraries were constructed from E12-13 embryos. After mass excision and isolation of plasmid DNA, approximately 4800 expressed sequence tag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9741
更新日期:2000-07-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00139-8
更新日期:2003-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.05.024
更新日期:2005-08-01 00:00:00
abstract::In the absence of maternal expression of the pecanex gene, the embryo develops severe hyperneuralization similar to that characteristic of Notch mutant embryos. We have extended a previous molecular analysis of the chromosomal interval that encompasses pecanex by using additional deficiencies to localize the locus on ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90127-9
更新日期:1989-11-01 00:00:00