Abstract:
:The clonal multipotential RCJ 3.1 cell line, which gives rise to myotubes, adipocytes, chondrocytes, and osteoblasts, contains different progenitor subpopulations. By limiting dilution analysis, of 296 single colonies identified, approximately 20% contained a single recognizable cell type, approximately 10% contained two cell types, and approximately 1% contained three cell types. We recloned RCJ 3.1 and isolated continuously growing subclones, including four novel bipotential (adipocytes/chondrocytes; adipocytes/myotubes and chondrocytes/myotubes) cell populations, whose phenotypes bred true. In the bipotential subclones, single colony analyses confirmed the presence of single cells which could both self-renew the bipotential progenitors and give rise to their respective committed monopotential lineages. Eight subclones were restricted to a single cell lineage and were considered monopotential; one of these is a novel cell line differentiating into cartilage. Thus, we have isolated unique monopotential and bipotential progenitor cell lines which provide a valuable model for studying the mechanisms leading to lineage restriction in mesenchymal populations.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Grigoriadis AE,Heersche JN,Aubin JEdoi
10.1016/0012-1606(90)90352-jsubject
Has Abstractpub_date
1990-12-01 00:00:00pages
313-8issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(90)90352-Jjournal_volume
142pub_type
杂志文章abstract::Eggs and embryos of the sea urchin Lytechinus pictus were labeled with [35S]methionine. Aqueous extracts of protein were prepared and analyzed by a high resolution two-dimensional polyacrylamide gel electrophoresis system described recently by O'Farrell utilizing isoelectric focusing and sodium dodecyl sulfate electro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(76)90248-7
更新日期:1976-09-01 00:00:00
abstract::The oblique muscle layer in the leech body wall is built upon the processes of a unique identified embryonic cell, the Comb- or C-cell. Each C-cell is composed of a spindle-shaped soma that projects approximately 70 parallel processes through the developing body wall at an angle oblique to the long axis. The morphogen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90342-z
更新日期:1991-11-01 00:00:00
abstract::For both the intricate morphogenetic layout of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2014.01.023
更新日期:2014-05-01 00:00:00
abstract::GATA4, a transcription factor expressed in the proximal small intestine but not in the distal ileum, maintains proximal-distal distinctions by multiple processes involving gene repression, gene activation, and cell fate determination. Friend of GATA (FOG) is an evolutionarily conserved family of cofactors whose member...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.07.022
更新日期:2008-10-01 00:00:00
abstract::A large subset of mammalian imprinted genes show extra-embryonic lineage (EXEL) specific imprinted expression that is restricted to placental trophectoderm lineages and to visceral yolk sac endoderm (ysE). Isolated ysE provides a homogenous in vivo model of a mid-gestation extra-embryonic tissue to examine the mechani...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.04.010
更新日期:2015-06-15 00:00:00
abstract::To determine whether spontaneous release of transmitter from the growth cones of neurons exhibits properties similar to the spontaneous release which occurs from the neurons at the neuromuscular junction, release of transmitter from the growth cones of Xenopus neurons in culture was monitored in salines containing var...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90172-7
更新日期:1986-02-01 00:00:00
abstract::Runx2-Cbfa1, a Runt transcription factor, plays important roles during skeletal development. It is required for differentiation and function of osteoblasts. In its absence, chondrocyte hypertrophy is severely impaired and there is no vascularization of cartilage templates during skeletal development. These tissue-spec...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.03.003
更新日期:2004-06-15 00:00:00
abstract::Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9659
更新日期:2000-06-15 00:00:00
abstract::Boundary formation is an important mechanism of development and has been studied in a number of bilaterian model organisms where it is often controlled by Notch, FGF and Wnt signalling. Tissue boundaries are also formed in simple pre-bilaterian animals. The boundary between parent and bud during asexual reproduction i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.05.517
更新日期:2010-08-01 00:00:00
abstract::Full-grown Xenopus oocytes, Stage VI (1200-1300 microns), undergo meiotic maturation when exposed to progesterone. Smaller stage IV (800 microns) and stage V (1000 microns) oocytes remain in prophase arrest when exposed to this steroid. The larger stage VI oocytes undergo an intracellular alkalization from 7.2 to 7.6,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90288-4
更新日期:1984-10-01 00:00:00
abstract::Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9727
更新日期:2000-06-15 00:00:00
abstract::Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.08.009
更新日期:2016-10-01 00:00:00
abstract::Nontransformed cultures of vascular smooth muscle cells proliferate until they form a confluent sheet of cells. Subsequently, the cells become reorganized to form multicellular nodules that are loosely attached to the substrate. The formation of nodules is facilitated by the addition of medium conditioned by nodular c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90095-7
更新日期:1983-06-01 00:00:00
abstract::Prestalk cells are found in the anterior 15% of slugs of Dictyostelium discoideum and prespore cells are found in the remaining posterior portions. Neutral red staining as well as immunostaining with antisera prepared against spores or a spore-coat protein has indicated that there are cells in the posterior region whi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90318-5
更新日期:1985-02-01 00:00:00
abstract::Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lap...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.03.016
更新日期:2019-06-15 00:00:00
abstract::The transcription factors, GATA4, 5 and 6, recognize the same DNA sequence and are all expressed in the developing myocardium. However, knockout studies in the mouse have indicated that none of them are absolutely required for the specification of the myocardium. Here we present evidence for redundancy in this family ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.08.018
更新日期:2007-11-15 00:00:00
abstract::We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90178-1
更新日期:1983-04-01 00:00:00
abstract::We have isolated a nonneuronal cell line from Xenopus retinal neuroepithelium (XR1 cell line). On the basis of immunocytochemical characterization using monoclonal antibodies generated in our laboratory as well as several other glial-specific antibodies, we have established that the XR1 cells are derived from embryoni...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90086-9
更新日期:1989-07-01 00:00:00
abstract::Nicastrin is a recently identified member of high-molecular weight complexes containing presenilin. The Caenorhabditis elegans homolog of nicastrin, aph-2, was shown to be required for GLP-1/Notch signaling in the early embryo. In addition to the maternal-effect embryonic lethal phenotype, aph-2 mutant animals also di...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0486
更新日期:2001-12-15 00:00:00
abstract::Meckel-Gruber syndrome (MKS) is an embryonic lethal ciliopathy resulting from mutations in genes encoding proteins localising to the primary cilium. Mutations in the basal body protein MKS1 account for 7% of cases of MKS. The condition affects the development of multiple organs, including brain, kidney and skeleton. H...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.02.015
更新日期:2013-05-01 00:00:00
abstract::In order to further our understanding of the biological role of desmin, the muscle-specific intermediate filament protein, a null mutation in the desmin gene was introduced into the germ line of mice. Despite the complete lack of desmin, these mice developed and reproduced. Since we show that skeletal, cardiac, and sm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0122
更新日期:1996-05-01 00:00:00
abstract::During development of the central nervous system (CNS), commissural axons grow toward the ventral midline. After crossing the floor plate, they abruptly change their trajectory from the circumferential to the longitudinal axis. The contacts between the commissural axons and the floor plate cells are involved in this a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.05.034
更新日期:2004-09-15 00:00:00
abstract::Spermatogenesis is initiated by the interaction of germ cells and somatic cells in seminiferous tubules. We used cDNA microarrays and representational difference analysis to identify genes that are expressed in the testis of the jsd/jsd mutant mouse, which contains only type A spermatogonial germ cells and Sertoli cel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0671
更新日期:2002-06-15 00:00:00
abstract::High osmolarity, bound water, and hydrostatic pressure contribute to notochord mechanics and its morphogenesis into the nucleus pulposus (NP) compartment of the intervertebral disc. Indeed, the osmoadaptive transcription factor, nuclear factor of activated T-cells 5 (NFAT5 aka TonEBP), is robustly expressed by residen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.004
更新日期:2019-11-15 00:00:00
abstract::The Drosophila Suppressor of Hairy wing [Su(Hw)] insulator protein has an essential role in the development of the female germline. Here we investigate the function of Su(Hw) in the ovary. We show that Su(Hw) is universally expressed in somatic cells, while germ cell expression is dynamic. Robust levels accumulate in ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.666
更新日期:2011-08-15 00:00:00
abstract::We have examined the roles of cell lineage and interactions in the determination of individual identified neurons in the grasshopper embryo by selective ablations of individual cells and/or their neighbors at successive stages following their birth. The neurons in the grasshopper central nervous system (CNS) are produ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90069-7
更新日期:1985-07-01 00:00:00
abstract::Cell surface and cytoplasmic polarity is exhibited by the blastomeres of mouse preimplantation embryos following compaction at the 8-cell stage of cleavage. It has been hypothesized that cytoplasmic polarity is initiated by plasma membrane functions of polar blastomeres that are absent from apolar blastomeres. To test...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90433-2
更新日期:1988-11-01 00:00:00
abstract::Summary Sentence: Conditional ablation of AP-2 gamma results in a delay in skin development and abnormal expression of p63, K14, K1, filaggrin, repetin and secreted Ly6/Plaur domain containing 1, key genes required for epidermal development and differentiation. The development of the epidermis, a stratified squamous e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.017
更新日期:2008-05-01 00:00:00
abstract::Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular ca...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9745
更新日期:2000-07-01 00:00:00
abstract::Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0257
更新日期:1996-10-10 00:00:00