Abstract:
:Previous studies from this laboratory have identified a novel alpha-D-mannosidase on the sperm plasma membranes of several species, including man, which may have a role in fertilization. The polyclonal antibody raised against an isoform of the enzyme purified from rat epididymal fluid was found to cross-react with the alpha-D-mannosidase activity present in the detergent-solubilized spermatozoa and sperm plasma membranes. In the present study, we have used affinity-purified as well as monospecific anti-mannosidase IgG to demonstrate that the sperm mannosidase is an integral plasma membrane component of the rat sperm and is localized on the periacrosomal region of the sperm head. In addition, we demonstrate proteolytic processing of the membrane-bound alpha-D-mannosidase during maturation of spermatozoa. The membrane fractions prepared from testis, and spermatozoa from the caput, corpus, and cauda regions of the epididymis, were solubilized in SDS and resolved by SDS-PAGE. The resolved polypeptides, when subjected to Western blot analysis using affinity-purified anti-mannosidase IgG as the primary antibody, revealed the presence of three specific immunoreactive bands (apparent M(r), 135, 125, and 115 kDa) in the membranes from testis, caput, and corpus spermatozoa. However, the cauda sperm plasma membranes showed only one immunoreactive band of apparent M(r) 115 kDa. The disappearance of the 135-and 125-kDa forms and the appearance of a sharp 115-kDa band on cauda spermatozoa suggests a precursor-product relationship between various molecular forms of the enzyme. Trypsin treatment of testicular and caput sperm membranes largely converted the precursor forms to the mature (115-kDa) form. The in vitro proteolysis resulted in an elevated level of the alpha-D-mannosidase activity in the caput (but not cauda) sperm plasma membrane. Inclusion of trypsin inhibitors (benzamidine and aprotinin) largely prevented the conversion of precursor form to the mature form. These data are consistent with the observed increase in the levels of sperm enzyme activity as spermatozoa move from the caput to the cauda region and suggest that the increase is due to the conversion of enzymatically inactive/less active high molecular weight precursor forms (135 and 125 kDa) into enzymatically active mature form (115 kDa) during sperm maturation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Tulsiani DR,NagDas SK,Skudlarek MD,Orgebin-Crist MCdoi
10.1006/dbio.1995.1050subject
Has Abstractpub_date
1995-02-01 00:00:00pages
584-95issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)71050-0journal_volume
167pub_type
杂志文章abstract::Regulation of peptide neurotransmitter metabolism was examined in dissociated cell cultures of neonatal rat sympathetic and sensory ganglia. Previous studies have shown that pineal gland conditioned medium (PCM) influences substance P (SP) and somatostatin (SS) metabolism in sympathetic neurons in vitro. The present s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90061-7
更新日期:1984-11-01 00:00:00
abstract::In Caenorhabditis elegans, Wnt signaling regulates many asymmetric cell divisions. During embryogenesis, the C. elegans Dishevelled (Dsh) homolog, DSH-2, regulates asymmetric neuroblast division of the ABpl/rpppa blast cell. Dsh is a key intracellular component of both beta-catenin dependent and beta-catenin independe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.01.025
更新日期:2009-04-15 00:00:00
abstract::A large subset of mammalian imprinted genes show extra-embryonic lineage (EXEL) specific imprinted expression that is restricted to placental trophectoderm lineages and to visceral yolk sac endoderm (ysE). Isolated ysE provides a homogenous in vivo model of a mid-gestation extra-embryonic tissue to examine the mechani...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.04.010
更新日期:2015-06-15 00:00:00
abstract::Fertilized eggs of the leech Helobdella triserialis undergo a cytoplasmic reorganization which generates domains of nonyolky cytoplasm, called teloplasm, at the animal and vegetal poles. The segregation of teloplasm to one cell of the eight-cell embryo is responsible for a unique developmental fate of that cell, i.e.,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90181-4
更新日期:1989-10-01 00:00:00
abstract::To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.007
更新日期:2007-02-01 00:00:00
abstract::The accumulation of total RNA transcripts of 18 late embryo-abundant (Lea) gene families, each encoding two closely related Lea mRNAs, was measured in cotyledon total RNA during embryogenesis and germination of Gossypium hirsutum L. by RNA dot hybridization. Transcript abundance of the three storage protein families w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90442-8
更新日期:1987-09-01 00:00:00
abstract::The pdx1 gene is essential for pancreatic organogenesis in humans and mice; pdx1 mutations have been identified in human diabetic patients. Specific inactivation of pdx1 in adult beta cells revealed that this gene is required for maintenance of mature beta cell function. In the following study, a Cre-lox strategy was ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.038
更新日期:2008-02-15 00:00:00
abstract::The current picture of embryonic development in nematodes is essentially shaped by Caenorhabditis elegans and its close relatives. As their pattern of embryogenesis is rather similar, it is often considered to be representative for the taxon Nematoda as a whole. Here we give for the first time a comprehensive descript...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.009
更新日期:2009-10-01 00:00:00
abstract::Vasa is a DEAD-box RNA helicase that functions in translational regulation of specific mRNAs. In many animals it is essential for germ line development and may have a more general stem cell role. Here we identify vasa in two sea urchin species and analyze the regulation of its expression. We find that vasa protein acc...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.039
更新日期:2008-02-15 00:00:00
abstract::A fundamental feature of cartilage differentiation in the developing limb is the formation of a prechondrogenic cell condensation. An apparently similar process of prechondrogenic cell aggregation occurs in micromass cultures of limb bud mesenchyme with the formation of cellular aggregates which often differentiate in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90314-9
更新日期:1987-08-01 00:00:00
abstract::Although mitogen-activated protein kinase (MAPK) is a well-known cell cycle regulator, emerging studies have also implicated its activity in the regulation of intracellular calcium concentration ([Ca2+](i)) and secretion. Those studies raise the hypothesis that MAPK activity during oocyte maturation and early fertiliz...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.029
更新日期:2007-06-15 00:00:00
abstract::Wnt signaling pathways have been demonstrated to play important roles in controlling tissue patterning and cell proliferation. In the gastrointestinal tract, mutations that lead to activation of the canonical Wnt pathway through beta-catenin result in familial and sporadic colon cancers. The downstream transcription f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00185-4
更新日期:2003-07-15 00:00:00
abstract::Repair and regeneration of adult skeletal muscle are mediated by satellite cells. In healthy muscle these rare mononucleate muscle precursor cells are mitotically quiescent. Upon muscle injury or degeneration, members of this self-renewing pool are activated to proliferate and then differentiate. Here we analyzed in s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8721
更新日期:1997-11-15 00:00:00
abstract::Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.02.021
更新日期:2014-06-01 00:00:00
abstract::To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.12.019
更新日期:2020-04-01 00:00:00
abstract::Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling. Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remai...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.033
更新日期:2010-08-01 00:00:00
abstract::The source of neurite outgrowth in explant cultures of normal adult Apteronotus spinal cord was examined. Explants which contained the central region of spinal cord, including ependyma, showed neurite outgrowth in culture. Explants which did not contain ependyma showed no neurite outgrowth. It is concluded that the ep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90062-5
更新日期:1987-02-01 00:00:00
abstract::The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.0025
更新日期:1995-11-01 00:00:00
abstract::The unc-52 gene of Claenorhabditis elegans encodes a homologue of the basement membrane heparan sulfate proteoglycan perlecan. Viable alleles reduce the abundance of UNC-52 in late larval stages and increase the frequency of distal tip cell (DTC) migration defects caused by mutations disrupting the UNC-6/netrin guidan...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00014-9
更新日期:2003-04-01 00:00:00
abstract::Sperm-egg interaction was investigated in mouse eggs freed from the zona pellucida and injected with Clostridium difficile toxin B, the inhibitor of Rho family small G proteins. Toxin B reduced in a dose-dependent manner the percentage of eggs associated with sperm fusion on the surface or sperm nucleus decondensation...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00273-2
更新日期:2003-08-15 00:00:00
abstract::Tabby is a mouse mutant characterized by deficient development of the ectodermal organs: teeth, hair, and a subset of glands. Ectodysplasin, the protein encoded by the Tabby gene, was recently identified as a novel TNF-like transmembrane protein but little is known about its function. We have examined the Tabby tooth ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9514
更新日期:1999-12-15 00:00:00
abstract::Using two different models of pregnancy failure in mice, we show in this communication that preimplantation embryonic development can be controlled, in vivo, with cytokines. In one model, described by us previously, CSF-1 is shown to block gestations in plugged females due to its induction of defective early developme...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90236-v
更新日期:1991-08-01 00:00:00
abstract::The neural cell adhesion molecule (NCAM) is thought to play a role in the formation of the vertebrate nervous system. In mammals and chicken, it is known that more than 100 different forms of the NCAM protein can be generated by alternative splicing of one primary transcript and it is possible that these different for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90276-m
更新日期:1992-01-01 00:00:00
abstract::Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) are present in high levels in most areas of the embryonic rodent brain. To begin to understand the role of these growth factors in brain development, the effects of aFGF and bFGF on dissociated cell cultures prepared from embryonic and ne...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90143-5
更新日期:1992-08-01 00:00:00
abstract::Early embryonic cells are capable of acquiring numerous developmental fates until they become irreversibly committed to specific lineages depending on intrinsic determinants and/or regional interactions. From fertilization to gastrulation, such pluripotent cells first increase in number and then turn to undergoing dif...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2019.04.014
更新日期:2019-08-15 00:00:00
abstract::Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.040
更新日期:2007-05-01 00:00:00
abstract::Aurora-C was first identified during screening for kinases expressed in mouse sperm and eggs. Herein, we report for the first time the precise subcellular localization of endogenous Aurora-C during male meiotic division. The localization of Aurora-C was analyzed by immunofluorescence staining on chromosome spreads of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.036
更新日期:2006-02-15 00:00:00
abstract::Eye cups from stage 14-28 (E2 to E5) chick and quail embryos consisting of neural retina, lens, and vitreous body were cultured for 1 or 2 days. These eyes expanded by proliferation of the retinal cells and the surface areas of the retinae increased several-fold. The area covered by ganglion cells and axons also expan...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90194-6
更新日期:1986-04-01 00:00:00
abstract::The epidermis forms a vital barrier composed of stratified keratinocytes and their differentiated products. One of these products, keratin K10, is critical to epidermal integrity, because mutations in k10 lead to abnormal blistering. For the normal expression of k10, differentiation-associated transcription factors C/...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9460
更新日期:1999-12-01 00:00:00
abstract::Hox genes can function as key drivers of segment identity, with Hox mutations in Drosophila often resulting in dramatic homeotic transformations. In addition, however, they can serve other essential functions. In mammals, the study of Hox gene roles in development is complicated by the presence of four Hox clusters wi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.03.020
更新日期:2018-06-15 00:00:00