Abstract:
:An abundant phosphoprotein with an apparent molecular mass of 65 kDa (p65) has been identified that is enriched in developing new macronuclei (or anlagen) isolated from the holotrichous ciliate, Tetrahymena thermophila. During early stages of macronuclear development, p65 is actively synthesized and deposited into young (4C) anlagen and is not found in micronuclei or parental macronuclei. p65 is not detected in older (8C) anlagen or in vegetatively growing or starved cells, and thus p65 is under stringent developmental control. In situ analyses, using polyclonal antibodies generated against p65, demonstrate that p65 undergoes a pronounced change in distribution during anlagen differentiation. Initially, anlagen are uniformly stained with these antibodies. However, following separation of conjugants, this staining pattern converts to one that is punctate and fragmented. As development proceeds, most, if not all, of these p65-based particles become peripherally located in anlagen and appear as well-defined vesicles surrounding a discrete central core of DNA of yet undetermined origin. This remarkable cytological distribution suggests an involvement of p65 in the elimination or processing of DNA during anlagen differentiation. If the above is correct, p65 provides the first inroad into the protein machinery involved in the well-known DNA rearrangements that characterize ciliate macronuclear development.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Madireddi MT,Davis MC,Allis CDdoi
10.1006/dbio.1994.1264subject
Has Abstractpub_date
1994-10-01 00:00:00pages
418-31issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71264-4journal_volume
165pub_type
杂志文章abstract::Temperature sex determining species offer a model for investigating how environmental cues become integrated to the regulation of patterning genes and growth, among bipotential gonads. Manipulation of steroid hormones has revealed the important role of aromatase in the regulation of the estrogen levels involved in tem...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0486
更新日期:2001-12-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00