Abstract:
:Temperature sex determining species offer a model for investigating how environmental cues become integrated to the regulation of patterning genes and growth, among bipotential gonads. Manipulation of steroid hormones has revealed the important role of aromatase in the regulation of the estrogen levels involved in temperature-dependent sex determination. Estradiol treatment counteracts the effect of male-promoting temperature, but the resulting ovarian developmental pattern differs from that manifested with the female-promoting temperature. Hypoplastic gonads have been reported among estradiol-treated turtles; however the estradiol effect on gonadal size has not been examined. Here we focused on the sea turtle Lepidochelys olivacea, which develops hypoplastic gonads with estradiol treatment. We studied the effect of estradiol on cell proliferation and on candidate genes involved in ovarian pattern. We found this effect is organ specific, causing a dramatic reduction in gonadal cell proliferation during the temperature-sensitive period. Although the incipient gonads resembled tiny ovaries, remodeling of the medullary cords and down-regulation of testicular factor Sox9 were considerably delayed. Contrastingly, with ovarian promoting temperature as a cue, exogenous estradiol induced the up-regulation of the ovary factor FoxL2, prior to the expression of aromatase. The strong expression of estrogen receptor alpha at the time of treatment suggests that it mediates estradiol effects. Overall results indicate that estradiol levels required for gonadal growth and to establish the female genetic network are delicately regulated by temperature.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Díaz-Hernández V,Marmolejo-Valencia A,Merchant-Larios Hdoi
10.1016/j.ydbio.2015.05.022subject
Has Abstractpub_date
2015-12-01 00:00:00pages
79-89issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(15)30005-1journal_volume
408pub_type
杂志文章abstract::Following their birth in olfactory placode, luteinizing hormone-releasing hormone (LHRH)-containing neurons migrate across the developing cribriform plate and form a dispersed population in the mammalian basal forebrain. The present study reveals the colocalization of unique glycoconjugate antigens (detected with mono...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1045
更新日期:1993-02-01 00:00:00
abstract::XX gonads differentiate into ovaries, a morphologic event evident by embryonic day 13.5 (E13.5) in mice. To identify early markers of oogenesis, sex-specific urogenital ridge cDNA libraries were constructed from E12-13 embryos. After mass excision and isolation of plasmid DNA, approximately 4800 expressed sequence tag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9741
更新日期:2000-07-01 00:00:00
abstract::The mammalian kidney collecting ducts are critical for water, electrolyte and acid-base homeostasis and develop as a branched network of tubular structures composed of principal cells intermingled with intercalated cells. The intermingled nature of the different collecting duct cell types has made it challenging to id...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.02.007
更新日期:2017-04-01 00:00:00
abstract::Tubes with distinct shapes and sizes are critical for the proper function of many tubular organs. Here we describe a unique phenotype caused by the loss of a novel, evolutionarily-conserved, Drosophila Smad-like protein, Expansion. In expansion mutants, unicellular and intracellular tracheal branches develop bubble-li...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.06.016
更新日期:2014-09-01 00:00:00
abstract::The morphology of skeletal tissues formed in each of the branchial arches of higher vertebrates is unique. In addition to these structures, which are derived from the neural crest, the crest-derived connective tissues and mesodermal muscles also form different patterns in each of the branchial arches. The objective of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90318-4
更新日期:1983-03-01 00:00:00
abstract::Developmental boundaries ensure that cells fated to participate in a particular structure are brought together or maintained at the appropriate locale within developing embryos. Parasegment grooves mark the position of boundaries that separate every segment of the Drosophila embryo into anterior and posterior compartm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.026
更新日期:2008-09-15 00:00:00
abstract::The relationship between ascorbic acid concentration and cellular transport mechanisms was studied in chicken embryos (Gallus gallus domesticus). Unincubated (Day 0) fertile eggs did not contain detectable levels of ascorbic acid as assayed by high performance liquid chromatography with electrochemical detection. Howe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90298-w
更新日期:1990-06-01 00:00:00
abstract::In normal development the veg1 tier of the sixth cleavage Strongylocentrotus purpuratus embryo contributes progeny to both ectodermal lineages and portions of the archenteron. Treatment with 18 mM LiCl specifically affects this lineage allocation, reducing or eliminating the veg1 contribution to ectoderm. Less frequen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8615
更新日期:1997-07-15 00:00:00
abstract::Pulses of ecdysteroids induce dramatic changes in gene expression that direct the early stages of Drosophila metamorphosis. This gene activity is reflected by the appearance of early and late puffs in the salivary gland polytene chromosomes. Curiously, the early puff genes that have been studied to date are induced by...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1262
更新日期:1995-09-01 00:00:00
abstract::The universal signal for egg activation at fertilization is a rise in cytoplasmic Ca(2+) with defined spatial and temporal kinetics. Mammalian and amphibian eggs acquire the ability to produce such Ca(2+) signals during a maturation period that precedes fertilization and encompasses resumption of meiosis and progressi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.08.004
更新日期:2004-11-01 00:00:00
abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
abstract::The polypeptide labeling patterns of somatic cells, gonidia (asexual reproductive cells), embryos, and juvenile spheroids of Volvox carteri cultures synchronized by a light/dark cycle were studied as a function of developmental stage and incubation condition. Specimens were exposed to 35SO=4 for 1-hr periods at select...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90186-0
更新日期:1983-04-01 00:00:00
abstract::Microtubule organization during the novel cell division of ameiotic microsporocytes was examined using indirect immunofluorescence microscopy. A recessive mutation of the maize gene Ameiotic causes the replacement of meiosis I with a synchronized mitotic division (Palmer, R. G. (1971). Chromosoma 35, 233-246). All ide...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90063-m
更新日期:1992-11-01 00:00:00
abstract::Initiation of transcription mediated by RNA polymerase II requires a number of transcription factors among which TFIID is the major core promoter recognition factor. TFIID is composed of highly conserved factors which include the TATA-binding protein (TBP) and about 14 TBP-associated factors (TAFs). Recently, the comp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.06.006
更新日期:2005-09-01 00:00:00
abstract::Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8963
更新日期:1998-08-15 00:00:00
abstract::Developmentally regulated gene expression has been analyzed in the wild-type D. discoideum strain NC-4 and a series of temporally deranged mutants. The mutants include representatives from each class of rapid development mutation, Fr17(rdeA-) and HT506(rdeC-), and strain HIfm-1, which appears to be defective in the ti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90260-5
更新日期:1986-06-01 00:00:00
abstract::Changes in the intracellular free calcium levels in the cortical region of cleaving Xenopus eggs were examined using semi-synthetic aequorin. We detected periodic oscillations in the free calcium levels accompanying the cleavage cycle as early as the first cleavage onward. The calcium levels began to increase at early...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1325
更新日期:1993-12-01 00:00:00
abstract::To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1α. On...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.08.017
更新日期:2010-12-01 00:00:00
abstract::Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiolog...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.11.003
更新日期:2015-02-15 00:00:00
abstract::The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8764
更新日期:1997-12-15 00:00:00
abstract::Animal development is controlled by gene regulation networks that are composed of sequence-specific transcription factors (TF) and cell signaling molecules (ST). Although housekeeping genes have been reported to show clustering in the animal genomes, whether the genes comprising a given regulatory network are physical...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.009
更新日期:2008-04-15 00:00:00
abstract::Localisation of Protein Kinase A (PKA) by A-Kinase Anchoring Proteins (AKAPs) is known to coordinate localised signalling complexes that target cAMP-mediated signalling to specific cellular sub-domains. The cAMP PKA signalling pathway is implicated in both meiotic arrest and meiotic resumption, thus spatio-temporal ch...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.045
更新日期:2008-05-01 00:00:00
abstract::The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1040
更新日期:1995-02-01 00:00:00
abstract::Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8619
更新日期:1997-08-01 00:00:00
abstract::Pancreatic development depends on the transcription factor Pax6, which controls islet cell differentiation and hormone production. To understand the regulation of Pax6 pancreatic expression, we have identified a minimal Pax6 pancreatic enhancer and show that it contains a composite binding site for Meis and Pbx homeop...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.030
更新日期:2006-12-15 00:00:00
abstract::The insect head is composed of several segments. During embryonic development, the segments fuse to form a rigid head capsule where obvious segmental boundaries are lacking. Hence, the assignment of regions of the insect head to specific segments is hampered, especially with respect to dorsal (vertex) and lateral (gen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.11.010
更新日期:2010-02-01 00:00:00
abstract::Neural crest cells migrate extensively through a complex extracellular matrix (ECM) to sites of terminal differentiation. To determine what role the various components of the ECM may play in crest morphogenesis, quail (Coturnix coturnix japonica) neural crest cells have been cultured in three-dimensional hydrated coll...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90094-0
更新日期:1984-08-01 00:00:00
abstract::Spontaneous germ cell death is a common cellular process in the mammalian testis, although the function of this process during spermatogenesis is unclear. An investigation was undertaken to determine whether p53 serves as a mechanism in germ cell quality control by causing spontaneous germ cell death. Using an annexin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9074
更新日期:1998-12-01 00:00:00
abstract::Collapsin-1 is a secreted glycoprotein that inhibits the extension of specific growth cones in vitro. It has been hypothesized to serve as a repulsive guidance cue for extending growth cones in vivo. Here we report the distribution of collapsin-1 message as demonstrated by in situ hybridization using digoxigenin-label...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0016
更新日期:1996-01-10 00:00:00
abstract::Genome stability relies notably on the integrity of centrosomes and on the mitotic spindle they organize. Structural and numerical centrosome aberrations are frequently observed in human cancer, and there is increasing evidence that centrosome amplification can promote tumorigenesis. Here, we use C. elegans seam cells...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.01.001
更新日期:2018-02-15 00:00:00