Abstract：
:The relationship between ascorbic acid concentration and cellular transport mechanisms was studied in chicken embryos (Gallus gallus domesticus). Unincubated (Day 0) fertile eggs did not contain detectable levels of ascorbic acid as assayed by high performance liquid chromatography with electrochemical detection. However, ascorbic acid concentration in brain increased to 5.6 nmol/mg tissue by Day 10 in ovo and then gradually declined 32% before birth. These levels were an order of magnitude greater than in skeletal muscle, where ascorbic acid concentration decreased sixfold between Days 8-20. Uptake of ascorbic acid was measured in brain cells that were either freshly isolated or grown in primary culture. Saturable, temperature- and Na(+)-dependent ascorbic acid transport was evident in freshly isolated cells as early as Day 6 and persisted throughout the period of ontogenic development. Primary cultures of embryonic chick brain cells were observed to take up ascorbic acid through a high-affinity (apparent Km = 37 microM, Vmax = 106 nmol ascorbic acid/g protein/min) mechanism. This transport system may maintain the high concentrations of ascorbic acid observed in the central nervous system during the ontogenic period when the levels of ascorbic acid in peripheral tissues change drastically.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wilson JXdoi
10.1016/0012-1606(90)90298-wsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
292-8issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(90)90298-Wjournal_volume
139pub_type
杂志文章abstract：:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0855
更新日期：2003-01-01 00:00:00
abstract：:Gastrulation in Xenopus laevis transforms a ball of undifferentiated cells into a well-organized elongated embryo with a distinct body axis, as a result of extensive cell rearrangements and movements. Since cell adhesion is thought to play an important role during tissue morphogenesis, we investigated the potential ro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1288
更新日期：1995-10-01 00:00:00
abstract：:The basic helix-loop-helix transcription factor Twist1 plays an essential role in mesenchymal cell populations during embryonic development and in pathological disease. Remodeling of the cardiac outflow tract (OFT) into the functionally separate aortic arch and pulmonary trunk is dependent upon the dynamic, coordinate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.037
更新日期：2008-08-01 00:00:00
abstract：:This manuscript reports on the limb malformations and axial skeleton alterations found in legless fetuses and their heterozygote and wild-type littermates transplacentally exposed to all-trans-retinoic acid via a single intraperitoneal injection on Day 7, 8, 9, 9.5, 10, 10.5, or 11 of gestation. The most surprising as...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1198
更新日期：1994-07-01 00:00:00
abstract：:The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development of Dictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90221-6
更新日期：1986-05-01 00:00:00
abstract：:Molecular mechanisms regulating the remodeling of the lymphatic vasculature from an immature plexus of vessels to a hierarchal network of initial and collecting lymphatics are not well understood. One gene thought to be important for this process is Angiopoietin-2 (Ang-2). Ang2(-/-) mice have previously been reported ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.024
更新日期：2008-07-15 00:00:00
abstract：:The formation of endocardial endothelium in quail embryos was investigated using in vivo and in vitro systems. Based on the expression of an quail endothelial marker, QH-1, the initial emergence of endothelial precursor cells in the embryo occurs at stage 7+ (two somites) in the posterior parts of the bilateral heart ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0096
更新日期：1996-04-10 00:00:00
abstract：:The notochord is the defining characteristic of the chordate embryo and plays critical roles as a signaling center and as the primitive skeleton. In this study we show that early notochord development in Xenopus embryos is regulated by apoptosis. We find apoptotic cells in the notochord beginning at the neural groove ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.047
更新日期：2007-11-15 00:00:00
abstract：:During heart formation, the heart grows and undergoes dramatic morphogenesis to achieve efficient embryonic function. Both in fish and amniotes, much of the growth occurring after initial heart tube formation arises from second heart field (SHF)-derived progenitor cell addition to the arterial pole, allowing chamber f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.11.008
更新日期：2021-02-01 00:00:00
abstract：:Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.040
更新日期：2007-05-01 00:00:00
abstract：:Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.04.017
更新日期：2019-08-15 00:00:00
abstract：:Developmental boundaries ensure that cells fated to participate in a particular structure are brought together or maintained at the appropriate locale within developing embryos. Parasegment grooves mark the position of boundaries that separate every segment of the Drosophila embryo into anterior and posterior compartm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.026
更新日期：2008-09-15 00:00:00
abstract：:Organogenesis relies on specific genetic and molecular programmes, which orchestrate growth and cellular differentiation over developmental time. This is particularly important during Drosophila eye development in which cell-cell inductive events and long-range signalling have to be integrated to regulate proper cell ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.10.011
更新日期：2015-12-01 00:00:00
abstract：:We have compared insulin binding by heart cells at 7 and 14 days of development. Species specificity, optimum pH, temperature relationships, and time to equilibrium for binding of insulin were the same in both 7 and 14-day systems. Curvilinear Scatchard plots for chicken insulin binding were demonstrated. Binding affi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(81)90410-3
更新日期：1981-06-01 00:00:00
abstract：:Chicken talpid(3) mutant embryos have a wide range of Hedgehog-signalling related defects and it is now known that the talpid(3) gene product encodes a novel protein essential for Hedgehog signalling which is required for both activator and repressor functions of Gli transcription factors (Davey, M.G., Paton, I.R., Yi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.017
更新日期：2007-01-01 00:00:00
abstract：:A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90049-0
更新日期：1983-02-01 00:00:00
abstract：:Oligodendrocyte precursor cells (OPCs) can be differentiated in culture into either oligodendrocytes or type-2 astrocytes (2As), depending on the culture conditions. Whereas oligodendrocyte development can occur in the absence of inducing signals, 2A development apparently cannot. Fetal calf serum (FCS) and bone morph...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.013
更新日期：2004-03-01 00:00:00
abstract：:The location and distribution of neural crest-derived Schwann cells during development of the peripheral nerves of chick forelimbs were examined using chick-quail chimeras. Neural crest cells were labeled by transplantation of the dorsal part of the neural tube from a quail donor to a chick host at levels of the neura...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90014-8
更新日期：1992-03-01 00:00:00
abstract：:Accumulating evidence indicates that the endometrial extracellular matrix (ECM) modulates trophoblast adhesion during mouse blastocyst implantation. In previous studies of adhesion-competent mouse blastocysts, we have demonstrated that integrin-mediated fibronectin (FN)-binding activity on the apical surface of tropho...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0644
更新日期：2002-05-15 00:00:00
abstract：:The patterns of myosin heavy chain (MyHC) isoform expression in the embryo and in the adult mouse are reasonably well characterized and quite distinct. However, little is known about the transition between these two states, which involves major decreases and increases in the expression of several MyHC genes. In the pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9974
更新日期：2001-01-15 00:00:00
abstract：:Oocytes from LTXBO mice exhibit a delayed entry into anaphase I and frequently enter interphase after the first meiotic division. This unique oocyte model was used to test the hypothesis that protein kinase C (PKC) may regulate the meiosis I-to-meiosis II transition. PKC activity was detected in LTXBO oocytes at proph...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0311
更新日期：2001-07-15 00:00:00
abstract：:Developmental pattern formation allows cells within a tissue or organ to coordinate their development and establish cell types in relationship to one another. To better characterize the developmental patterning events within one organ, the C. elegans hindgut, we have analyzed the expression pattern of several genes us...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00352-x
更新日期：2003-10-01 00:00:00
abstract：:This paper reports a preliminary in silico analysis of the sea urchin kinome. The predicted protein kinases in the sea urchin genome were identified, annotated and classified, according to both function and kinase domain taxonomy. The results show that the sea urchin kinome, consisting of 353 protein kinases, is close...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.074
更新日期：2006-12-01 00:00:00
abstract：:The definitive endoderm forms during gastrulation and is rapidly transformed into the gut tube which is divided along the anterior-posterior axis into the foregut, midgut, and hindgut. Lineage tracing and genetic analysis have examined the origin of the definitive endoderm during gastrulation and demonstrated that the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.10.040
更新日期：2010-01-15 00:00:00
abstract：:We report periodic movements during erection of Dictyostelium discoideum (Dd) sorocarps. Our observations lead to the working hypothesis that Dd sorocarp erection occurs by two superimposed processes: one periodic, with a modal period of 6 1/2 min, and one continuous. We tentatively identify the periodic process with ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(76)90237-2
更新日期：1976-09-01 00:00:00
abstract：:Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.12.008
更新日期：2004-04-01 00:00:00
abstract：:We report here the identification and characterization of a novel gene, T1 alpha, expressed in high abundance in adult rat lung, fetal lung, and early fetal brain. T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. The cDNA for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1024
更新日期：1995-01-01 00:00:00
abstract：:TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.025
更新日期：2014-12-01 00:00:00
abstract：:One rapid response of starfish oocytes to the maturation-inducing hormone, 1-methyladenine (1-MA), is the formation of transient actin-filled spikes on the cell surface. The presence and distribution of G- and F-actin and several actin-associated proteins were examined in cortices isolated from oocytes before, during,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90140-4
更新日期：1984-02-01 00:00:00
abstract：:Polychaete annelids and arthropods are both segmented protostome invertebrates. To investigate whether the segmented body plan of these two phyla share a common molecular ground pattern, we report the developmental expression of orthologues of the arthropod segment polarity genes engrailed (en), hedgehog (hh), and win...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.025
更新日期：2006-01-01 00:00:00
