Abstract:
:The polypeptide labeling patterns of somatic cells, gonidia (asexual reproductive cells), embryos, and juvenile spheroids of Volvox carteri cultures synchronized by a light/dark cycle were studied as a function of developmental stage and incubation condition. Specimens were exposed to 35SO=4 for 1-hr periods at selected intervals throughout the asexual life cycle; proteins were then extracted and analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by fluorography. Although sulfation accounts for more than half the 35S incorporated, the conditions of extraction and electrophoresis employed resulted in exclusion of most sulfated products and inclusion of nearly all products bearing incorporated sulfur amino acids in the electrophoretic analysis. Hence SDS-PAGE profiles reflect relative rates of synthesis of major polypeptides. The first phase of these studies involved examination of stage-specific differences in protein synthetic patterns. Because a single developmental stage exhibits different protein synthetic patterns in light and darkness, detailed developmental comparisons were made only on organisms or cells exposed to label in the light. They yielded the following results: Shortly after the completion of embryogenesis (while all cells are still linked by numerous cytoplasmic bridges) presumptive somatic cells and gonidia exhibit a nearly identical pattern of labeling of the major polypeptides. In just a few hours, however, as cytoplasmic bridges begin to break down, the synthetic patterns of the two cell types begin to diverge; with passing time this divergence becomes progressively greater. By the time gonidia are mature, the patterns of labeling of major polypeptides by somatic cells and gonidia exhibit far more differences than similarities. Embryos derived from these mature gonidia then exhibit numerous, reproducible, stage-specific changes in polypeptide labeling throughout embryogenesis. However, two glycoproteins that previous authors implicated in the control of the differentiative cleavage division are here shown to be labeled in the parental somatic cells, not in the embryos as was previously supposed; hence a central role for them in embryonic development seems highly unlikely. In the second phase of this study the effects of light on protein synthetic patterns of organisms at selected developmental stages were analyzed. At all stages marked, rapid, reversible changes in the pattern of labeling of major polypeptides occur when cultures are transferred from light to dark or vice versa, but these changes are most marked in juvenile spheroids at the end of the dark period during which they had completed their embryogenesis. Some, but by no means all, of the changes induced by light can be attributed to stimulated synthesis of chloroplast proteins, on both chloroplast and cytosol ribosomes. The proteins made at the beginning of one light period are not identical to those made at the end of the preceding light period...
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kirk DL,Kirk MMdoi
10.1016/0012-1606(83)90186-0subject
Has Abstractpub_date
1983-04-01 00:00:00pages
493-506issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90186-0journal_volume
96pub_type
杂志文章abstract::It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8773
更新日期:1997-12-15 00:00:00
abstract::The heart is the first organ to form and function in the vertebrate embryo. Furthermore, differences between the left and right sides of the embryo become first detectable during cardiac development. We observed strong cardiac laterality phenotypes in medaka embryos by manipulating Groucho protein activity. The phenot...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.11.020
更新日期:2007-03-01 00:00:00
abstract::The vertebral column is derived from somites, which are transient segments of the paraxial mesoderm that are present in developing vertebrates. The strict spatial and temporal regulation of somitogenesis is of crucial developmental importance. Signals such as Wnt and FGF play roles in somitogenesis, but details regard...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.12.034
更新日期:2007-04-15 00:00:00
abstract::Choriogenesis (eggshell formation) within the silkmoth Antheraea polyphemus proceeds in parallel for the two major subpopulations of follicle cells, diverging only during the very late period when aeropyle crown surface structures form in one region but not in the other. Correlated with their appearance is the synthes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1301
更新日期:1993-11-01 00:00:00
abstract::Despite differences in the formation of neural precursors, all arthropod species analyzed so far generate about 30 single precursors (insects/crustaceans) or precursor groups (chelicerates/myriapods) per hemi-segment. In Drosophila, each precursor has a distinct identity conferred by segment polarity and dorso-ventral...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.029
更新日期:2006-02-15 00:00:00
abstract::Neuronal circuit development and function require proper synapse formation and maintenance. Genetic screens are one powerful method to identify the mechanisms shaping synaptic development and stability. However, genes with essential roles in non-neural tissues may be missed in traditional loss-of-function screens. In ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.04.008
更新日期:2012-06-15 00:00:00
abstract::Choroideremia (CHM) is a hereditary eye disease caused by mutations in the X-linked CHM gene. Disruption of the Chm gene in mice resulted in prenatal death of Chm-/Y males and Chm-/Chm+ females that had inherited the mutation from their mothers. Male chimeras and Chm+/Chm- females with paternal transmission of the mut...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.016
更新日期:2004-08-01 00:00:00
abstract::Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.042
更新日期:2009-09-15 00:00:00
abstract::A new monoclonal antibody, specific to an epitope in the carboxyl terminus of the Drosophila collagen IV molecule (basement membrane collagen) was identified. The distributions of collagen IV, laminin, and an additional extracellular molecule, the 2G2 antigen (2G2-Ag), were followed immunocytochemically during early w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1068
更新日期:1995-03-01 00:00:00
abstract::Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.05.006
更新日期:2020-08-01 00:00:00
abstract::Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90479-9
更新日期:1987-11-01 00:00:00
abstract::The engrailed gene is well known from its role in segmentation and central nervous system development in a variety of species. In molluscs, however, engrailed is involved in shell formation. So far, it seemed that engrailed had been co-opted uniquely for this particular process in molluscs. Here, we show that, in the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0653
更新日期:2002-06-15 00:00:00
abstract::The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1040
更新日期:1995-02-01 00:00:00
abstract::Here we describe a novel set of peptidergic neurons conserved throughout all developmental stages in the Drosophila central nervous system (CNS). We show that a small complement of 28 apterous-expressing cells (Ap-let neurons) in the ventral nerve cord (VNC) of Drosophila larvae co-express numerous gene products. The ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.015
更新日期:2004-05-01 00:00:00
abstract::Neurogenin (Ngn) 1 and Ngn2 encode basic-helix-loop-helix transcription factors expressed in the developing neocortex. Like other proneural genes, Ngns participate in the specification of neural fates and neuronal identities, but downstream effectors remain poorly defined. We set out to identify Ngn2 effectors in the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.06.013
更新日期:2004-09-15 00:00:00
abstract::Myogenic precursors in adult skeletal muscle (satellite cells) are mitotically quiescent but can proliferate in response to a variety of stresses including muscle injury. To gain further understanding of adult myoblasts, we analyzed myogenesis of satellite cells on intact fibers isolated from adult rat muscle. In this...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1226
更新日期:1994-08-01 00:00:00
abstract::Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lap...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.03.016
更新日期:2019-06-15 00:00:00
abstract::The Mix/Bix family of paired-like homeobox genes encode evolutionarily conserved, sequence specific, DNA-binding transcription factors that have been implicated in the co-ordination of gene expression, axis formation and cell fate determination during gastrulation in vertebrates. When mutated, these genes give rise to...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2012.04.033
更新日期:2012-07-15 00:00:00
abstract::The GATA family of transcription factors are known to play multiple critical roles in vertebrate developmental processes, including erythropoiesis, endoderm formation and cardiogenesis. There have been no previous demonstrations of a functional role for any GATA family member being associated with musculoskeletal deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.12.001
更新日期:2008-02-15 00:00:00
abstract::This study was undertaken to determine whether premigratory neural crest cells of the axolotl embryo differentiate autonomously into chromatophores, or whether stimuli from the environment, particularly from the extracellular matrix, are required for this process. Neural crest cells were excised from the dorsal part o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90168-5
更新日期:1986-02-01 00:00:00
abstract::In embryos of Patella vulgata at the 32-cell stage, one of the four vegetally located macromeres makes contacts with overlying animal micromeres. As a result, this macromere (designated 3D) divides significantly later than the other macromeres and forms the mesodermal stem cell 4d. Shortly before and during this inter...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90239-x
更新日期:1989-04-01 00:00:00
abstract::Major insulin gene transcription factors, such as PDX-1 or NeuroD1, have equally important roles in pancreatic development and the differentiation of pancreatic endocrine cells. Previously, we identified and cloned another critical insulin gene transcription factor MafA (RIPE3b1) and reported that other Maf factors we...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.028
更新日期:2006-05-15 00:00:00
abstract::It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90230-e
更新日期:1992-05-01 00:00:00
abstract::Alveologenesis is the final step of lung maturation, which subdivides the alveolar region of the lung into smaller units called alveoli. Each of the nascent dividers serves as a new gas-exchange surface, and collectively they drastically increase the surface area for breathing. Disruption of alveologenesis results in ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.11.017
更新日期:2016-01-15 00:00:00
abstract::The adult fungiform taste papilla is a complex of specialized cell types residing in the stratified squamous tongue epithelium. This unique sensory organ includes taste buds, papilla epithelium and lateral walls that extend into underlying connective tissue to surround a core of lamina propria cells. Fungiform papilla...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.07.022
更新日期:2013-10-01 00:00:00
abstract::The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.10.006
更新日期:2011-12-15 00:00:00
abstract::Cellular dedifferentiation underlies topical issues in biology such as regeneration and nuclear cloning and has common features in plants and animals. In plants, this process characterizes the transition of differentiated leaf cells to protoplasts (plant cells devoid of cell walls) and is accompanied by global chromat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.023
更新日期:2007-06-15 00:00:00
abstract::During primary neurogenesis in Xenopus, a cascade of helix--loop--helix (HLH) transcription factors regulates neuronal determination and differentiation. While XNeuroD functions at a late step in this cascade to regulate neuronal differentiation, the factors that carry out terminal differentiation are still unknown. W...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0230
更新日期:2001-05-15 00:00:00
abstract::Plant growth and development are driven by extended phases of irreversible cell expansion generating cells that increase in volume from 10- to 100-fold. Some specialized cell types define cortical sites that reinitiate polarized growth and generate branched cell morphology. This structural specialization of individual...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2018.12.004
更新日期:2019-07-01 00:00:00
abstract::Proliferating intestinal stem cells (ISCs) generate all cell types of the Drosophila midgut, including enterocytes, endocrine cells, and gland cells (e.g., copper cells), throughout the lifetime of the animal. Among the signaling mechanisms controlling the balance between ISC self-renewal and the production of differe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.06.018
更新日期:2016-08-15 00:00:00