Abstract:
:We have used in situ hybridization and RNA blotting analysis to compare the timing of accumulation of poly(A) and alpha-subtype histone mRNA during oogenesis in the sea urchin Strongylocentrotus purpuratus. In situ hybridization with 3H-poly(U) shows that the content of poly(A) in the developing oocyte increases four- to sixfold during vitellogenesis, implying a similar increase for polyadenylated maternal RNAs. In contrast, both RNA blotting and in situ hybridization demonstrate that there is little, if any, alpha-subtype histone mRNA in large oocytes. These results suggest that these maternal mRNAs accumulate in the pronucleus of the haploid egg after completion of meiotic maturation where they are stored until their release during the breakdown of the pronucleus during prophase.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Angerer LM,DeLeon DV,Angerer RC,Showman RM,Wells DE,RafF RAdoi
10.1016/0012-1606(84)90161-1subject
Has Abstractpub_date
1984-02-01 00:00:00pages
477-84issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(84)90161-1journal_volume
101pub_type
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