Abstract:
:Hoxa2 gene is a primary player in regulation of craniofacial programs of head development in vertebrates. Here we investigate the evolution of a Hoxa2 neural crest enhancer identified originally in mouse by comparing and contrasting the fugu hoxa2a and hoxa2b genes with their orthologous teleost and mammalian sequences. Using sequence analyses in combination with transgenic regulatory assays in zebrafish and mouse embryos we demonstrate subfunctionalization of regulatory activity for expression in hindbrain segments and neural crest cells between these two fugu co-orthologs. hoxa2a regulatory sequences have retained the ability to mediate expression in neural crest cells while those of hoxa2b include cis-elements that direct expression in rhombomeres. Functional dissection of the neural crest regulatory potential of the fugu hoxa2a and hoxa2b genes identify the previously unknown cis-element NC5, which is implicated in generating the differential activity of the enhancers from these genes. The NC5 region plays a similar role in the ability of this enhancer to mediate reporter expression in mice, suggesting it is a conserved component involved in control of neural crest expression of Hoxa2 in vertebrate craniofacial development.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
McEllin JA,Alexander TB,Tümpel S,Wiedemann LM,Krumlauf Rdoi
10.1016/j.ydbio.2015.11.006subject
Has Abstractpub_date
2016-01-15 00:00:00pages
530-42issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(15)30225-6journal_volume
409pub_type
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