Abstract：
:Chick embryonic heart cell isolates and monolayer cultures were prepared from atria and ventricles at selected stages of cardiac development. The cardiac myocytes were assayed for myosin heavy chain (MHC) content using monoclonal antibodies (McAbs) specific in the heart for atrial (B-1), ventricular (ALD-19), or conductive system (ALD-58) isoforms. Using immunofluorescence microscopy or radioimmunoassay, MHC accumulation was measured before plating and at 48 hr or 7 days in culture. Reproducible changes in MHC antigenicity were observed by 7 days in both atrial and ventricular cultures. The changes were stage dependent and tissue specific but generally resulted in a decreased reactivity with the tissue specific MHC McAbs. In addition, the isoform recognized by ALD-58, characteristic of the conductive system cells in vivo, was never present in cultured myocytes. These results indicate that MHC isoforms produced in vivo may be replaced in monolayer cultures by an isoform(s) not recognized by our tissue specific MHC McAbs. This suggests that the intrinsic program of cardiac myogenesis, within cardiac myocytes, may not be sufficient to establish and maintain differential expression of tissue specific MHC in monolayer cell culture.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zadeh BJ,González-Sánchez A,Fischman DA,Bader DMdoi
10.1016/0012-1606(86)90241-1subject
Has Abstractpub_date
1986-05-01 00:00:00pages
204-14issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(86)90241-1journal_volume
115pub_type
杂志文章abstract：:Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They ...
journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:Transgenic mice expressing stabilized beta-catenin in neural progenitors develop enlarged brains resulting from increased progenitor expansion. To more precisely define beta-catenin regulation of progenitor fate, we employed a conditional transgenic approach to delete the beta-catenin regulatory domain from neural pro...
journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.012
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1080
更新日期：1995-04-01 00:00:00
abstract：:Developing cardiac myocytes divide a limited number of times before they stop and terminally differentiate, but the mechanism that stops their division is unknown. To help study the stopping mechanism, we defined conditions under which embryonic rat cardiac myocytes cultured in serum-free medium proliferate and exit t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9524
更新日期：1999-12-15 00:00:00
abstract：:Initiation of the development of the anterior-posterior axis in the mouse embryo has been thought to take place only when the anterior visceral endoderm (AVE) emerges and starts its asymmetric migration. However, expression of Lefty1, a marker of the AVE, was recently found to initiate before embryo implantation. This...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.020
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1999-03-01 00:00:00
abstract：:T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.013
更新日期：2010-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.004
更新日期：2013-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.666
更新日期：2011-08-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.042
更新日期：2010-04-15 00:00:00
abstract：:Myoblast fusion (a critical process by which muscles grow) occurs in a multi-step fashion that requires actin and membrane remodeling; but important questions remain regarding the spatial/temporal regulation of and interrelationship between these processes. We recently reported that the Rho-GAP, GRAF1, was particularl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.025
更新日期：2014-09-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.006
更新日期：2019-12-01 00:00:00
abstract：:The effect of hydrocortisone on the development of dorsal skin was analyzed in the chick embryo by (1) transmission electron microscopy, (2) indirect immunofluorescence histology of extracellular matrix components (collagen types I, III, and IV; fibronectin; and laminin), and (3) quantitative determination of collagen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90056-3
更新日期：1984-11-01 00:00:00
abstract：:The development of supernumerary bristle precursors induced by the mutation shaggy (sgg; also known as zeste-white 3) was examined in the developing wing blade of imaginal and pupal Drosophila. sgg clones were induced by mitotic recombination; clones were marked using enhancer-trap flies which express beta-galactosida...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1992-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.024
更新日期：2008-07-15 00:00:00
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journal_title：Developmental biology
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更新日期：2020-06-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1984-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2003-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:During organogenesis, Sonic hedgehog (Shh) possesses dual functions: Shh emanating from midline structures regulates the positioning of bilateral structures at early stages, whereas organ-specific Shh locally regulates organ morphogenesis at later stages. The mesonephros is a transient embryonic kidney in amniote, whe...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1987-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90039-8
更新日期：1983-02-01 00:00:00
abstract：:In Drosophila, wing hairs are aligned in a distally oriented, parallel array. The frizzled pathway determines proximal-distal cell polarity in the wing; however, in frizzled pathway mutants, wing hairs remain parallel. How wing hairs align has not been determined. We have demonstrated a novel role for the septate junc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.040
更新日期：2004-11-15 00:00:00
