Abstract:
:We describe the characterization and a functional analysis in Xenopus development of RalB, a small G protein. RalB RNA and protein are detectable during oogenesis and early development, but the gene is expressed only weakly in adult tissues. The RalB transcripts are processed by poly(A) extension during oocyte maturation and up to the gastrulation stage. Microinjection of wild-type or mutant RalB RNAs was performed in fertilized eggs in order to gain insight into the function of RalB during development. We show that during cleavage stages the activated GTP form of RalB specifically induces a cortical reaction that affects the localization of pigment granules. The use of different drugs suggests that this reaction is dependent on the outer cortical actin array. The relation between F-actin and RalB was shown by confocal analysis. Injection of mRNAs encoding the mutated activated form of RalB leads, at dependent doses, to a blocking of gastrulation or defects in closing of neural folding structures. In contrast, the inactivated form blocks only the closing of neural tube. Altogether, these observations suggest that RalB is part of a regulatory pathway that may affect the blastomere cytoskeleton and take part in early development.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Moreau J,Lebreton S,Iouzalen N,Mechali Mdoi
10.1006/dbio.1999.9254subject
Has Abstractpub_date
1999-05-15 00:00:00pages
268-81issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(99)99254-0journal_volume
209pub_type
杂志文章abstract::Metazoan development involves a myriad of dynamic cellular processes that require cytoskeletal function. Nonmuscle myosin II plays essential roles in embryonic development; however, knowledge of its role in post-embryonic development, even in model organisms such as Drosophila melanogaster, is only recently being reve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.06.028
更新日期:2010-09-15 00:00:00
abstract::Leaf primordia, first visible as small bumps, are produced in a cyclical pattern at the edges of the shoot apex, a smooth region at the top of the stem. Their formation is a biomechanical process. This review first considers hypothetical construction mechanisms and then summarizes research that provides information ab...
journal_title:Developmental biology
pub_type: 杂志文章,评审
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abstract::In many organisms early embryogenesis is characterised by a period refractory to transcription. In Caenorhabditis elegans, the one-cell embryo is transcriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lineage. This model suggests that histone tail modifications associated ...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
abstract::Mutations in the NF1 gene may cause developmental abnormalities and the formation of a variety of tumors of neural crest origin in humans. The NF1 gene codes for a large protein, neurofibromin (nf), which is structurally and functionally related to yeast and human ras-GTPase-activating proteins (ras-GAPs). Recently, t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1052
更新日期:1994-02-01 00:00:00
abstract::The morphogenetic processes responsible for the initial phase of gastrulation in sea urchin embryos are not known. Here we report observations of the size and position of clones of cells derived from horseradish peroxidase (HRP)-injected mesomeres and macromeres. The displacement of these clones during the initial pha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90255-2
更新日期:1991-08-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.04.010
更新日期:2018-08-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.019
更新日期:2013-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90112-v
更新日期:1990-09-01 00:00:00
abstract::Summary Sentence: Conditional ablation of AP-2 gamma results in a delay in skin development and abnormal expression of p63, K14, K1, filaggrin, repetin and secreted Ly6/Plaur domain containing 1, key genes required for epidermal development and differentiation. The development of the epidermis, a stratified squamous e...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.03.017
更新日期:2018-06-01 00:00:00
abstract::High osmolarity, bound water, and hydrostatic pressure contribute to notochord mechanics and its morphogenesis into the nucleus pulposus (NP) compartment of the intervertebral disc. Indeed, the osmoadaptive transcription factor, nuclear factor of activated T-cells 5 (NFAT5 aka TonEBP), is robustly expressed by residen...
journal_title:Developmental biology
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更新日期:2019-11-15 00:00:00
abstract::Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we ana...
journal_title:Developmental biology
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doi:10.1016/j.ydbio.2017.05.019
更新日期:2017-08-01 00:00:00
abstract::Oocytes from LTXBO mice exhibit a delayed entry into anaphase I and frequently enter interphase after the first meiotic division. This unique oocyte model was used to test the hypothesis that protein kinase C (PKC) may regulate the meiosis I-to-meiosis II transition. PKC activity was detected in LTXBO oocytes at proph...
journal_title:Developmental biology
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doi:10.1006/dbio.2001.0311
更新日期:2001-07-15 00:00:00
abstract::During development, cells craft an impressive array of actin-based structures, mediating events as diverse as cytokinesis, apical constriction, and cell migration. One challenge is to determine how cells regulate actin assembly and disassembly to carry out these cell behaviors. During Drosophila oogenesis diverse cell...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.030
更新日期:2009-09-01 00:00:00
abstract::In the vertebrate head, crucial parts of the sense organs and sensory ganglia develop from special regions, the cranial placodes. Despite their cellular and functional diversity, they arise from a common field of multipotent progenitors and acquire distinct identity later under the influence of local signalling. Here ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2012.06.028
更新日期:2012-10-01 00:00:00
abstract::Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8592
更新日期:1997-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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doi:10.1016/j.ydbio.2007.05.001
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90047-7
更新日期:1983-02-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-15 00:00:00
abstract::Prestalk cells are found in the anterior 15% of slugs of Dictyostelium discoideum and prespore cells are found in the remaining posterior portions. Neutral red staining as well as immunostaining with antisera prepared against spores or a spore-coat protein has indicated that there are cells in the posterior region whi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90318-5
更新日期:1985-02-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.022
更新日期:2011-01-15 00:00:00
abstract::XX gonads differentiate into ovaries, a morphologic event evident by embryonic day 13.5 (E13.5) in mice. To identify early markers of oogenesis, sex-specific urogenital ridge cDNA libraries were constructed from E12-13 embryos. After mass excision and isolation of plasmid DNA, approximately 4800 expressed sequence tag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9741
更新日期:2000-07-01 00:00:00
abstract::We have identified a cellular efflux pump, RhT, with the properties of an MDR transporter-a type of ATP-binding cassette transporter whose substrates include small hydrophobic molecules. RhT transports rhodamine 123 (Rh123) and is inhibited by low temperature, energy poisons, and several MDR transport inhibitors, such...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9611
更新日期:2000-04-01 00:00:00
abstract::The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.06.019
更新日期:2015-09-01 00:00:00
abstract::Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.06.017
更新日期:2014-09-01 00:00:00
abstract::beta-Catenin was originally identified biochemically as a protein that binds E-cadherin in cultured cells and that interaction was later shown to be essential for cadherin function. Independently, armadillo, the beta-catenin homolog in Drosophila melanogaster, was identified as a segment polarity gene necessary for th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8739
更新日期:1997-12-15 00:00:00