Abstract:
:Expression of the c-abl proto-oncogene in the mouse testis is characterized by the production of a unique 4.7-kb transcript present in germ cells that have entered the haploid phase of spermatogenesis. A similar developmental stage specificity of expression is observed for a member of the 70-kDa heat-shock protein (hsp 70) gene family. A unique-sized hsp 70 transcript (T-hsp 70) is produced in haploid spermatids and is stable throughout spermatogenesis. In the present study, we examined the regulation of expression of these genes by examining their association with polyribosomes. The germ cell-specific c-abl and T-hsp 70 mRNA variants were both associated with the polysomal fractions of mouse testis cells, suggesting that they are functional mRNAs. However, both c-abl and T-hsp 70 mRNAs were also found in the ribonucleoprotein particle fractions. The distribution of these mRNAs in both the polysomal and nonpolysomal fractions is comparable to that seen for the mRNA of protamine-1, a gene whose expression in the testis is known to be regulated at the level of translation. In contrast, transcripts from the beta-tubulin gene were seen predominantly in the polyribosomal fractions. These findings suggest that translation of the novel c-abl and T-hsp 70 transcripts is confined to subpopulations of testicular cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zakeri ZF,Ponzetto C,Wolgemuth DJdoi
10.1016/0012-1606(88)90222-9subject
Has Abstractpub_date
1988-02-01 00:00:00pages
417-22issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(88)90222-9journal_volume
125pub_type
杂志文章abstract::Mesenchymal-epithelial interactions are an important source of information for pattern formation during organogenesis. In the developing excretory system, one of the secreted mesenchymal factors thought to play a critical role in patterning the growth and branching of the epithelial ureteric bud is GDNF. We have teste...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.04.008
更新日期:2005-07-01 00:00:00
abstract::Neural stem cells are present in specific regions of the adult central nervous system (CNS). Recent evidence suggests that the ciliary epithelium (CE), a CNS derivative, in the adult mammalian eye, harbors a quiescent population of neural stem cells. Here, we report the identification of c-Kit signaling as one of the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.05.023
更新日期:2004-09-01 00:00:00
abstract::A series of chimeras was generated by injecting single normal, parthenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected by in situ ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80028-4
更新日期:1989-02-01 00:00:00
abstract::Congenital bony syngnathia, a rare but severe human birth defect, is characterized by bony fusion of the mandible to the maxilla. However, the genetic mechanisms underlying this birth defect are poorly understood, largely due to limitation of available animal models. Here we present evidence that transgenic expression...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.04.013
更新日期:2014-07-15 00:00:00
abstract::Large steady electrical currents traverse Drosophila follicles in vitro as well as permeabilized eggs. During the period of main follicle growth (stages 9-11), these currents enter the anterior or nurse cell end of the follicles. This inward current acts like a sodium ion influx with some calcium involvement. During t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90013-2
更新日期:1985-03-01 00:00:00
abstract::We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1236
更新日期:1993-09-01 00:00:00
abstract::An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.011
更新日期:2011-06-01 00:00:00
abstract::Oocyte maturation (meiosis reinitiation) in starfish is induced by the natural hormone 1-methyladenine (1-MeAde). Oocytes of Evasterias troschelii contain 0.43 pmole cyclic AMP/mg protein and 0.47 pmole cyclic GMP/mg protein. Upon stimulation by 1-MeAde the oocytes undergo a moderate (10-30%) decrease in their cAMP co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90166-7
更新日期:1987-06-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.12.016
更新日期:2005-03-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.015
更新日期:2008-08-01 00:00:00
abstract::Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90275-0
更新日期:1983-09-01 00:00:00
abstract::Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90157-1
更新日期:1988-09-01 00:00:00
abstract::Much progress has been made in elucidating the molecular networks required for specifying retinal cells, including photoreceptors, but the downstream mechanisms that maintain identity and regulate differentiation remain poorly understood. Here, we report that the transcription factor Glass has a dual role in establish...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.04.012
更新日期:2016-06-01 00:00:00
abstract::Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90201-0
更新日期:1986-04-01 00:00:00
abstract::The extracellular matrix of Xenopus laevis oocytes was analyzed before and after meiotic maturation using quick-freeze, deep-etch, rotary-shadow electron microscopy. The perivitelline space (PS) of the meiotically immature oocyte contains a filamentous network which connects microvilli (MV) and follicle cell macrovill...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80044-2
更新日期:1989-01-01 00:00:00
abstract::High osmolarity, bound water, and hydrostatic pressure contribute to notochord mechanics and its morphogenesis into the nucleus pulposus (NP) compartment of the intervertebral disc. Indeed, the osmoadaptive transcription factor, nuclear factor of activated T-cells 5 (NFAT5 aka TonEBP), is robustly expressed by residen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.004
更新日期:2019-11-15 00:00:00
abstract::Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1068
更新日期:1994-03-01 00:00:00
abstract::Epithelial sheet movement is an essential morphogenetic process during mouse embryonic eyelid closure in which Mitogen-Activated Protein 3 Kinase 1 (MAP3K1) and c-Jun play a critical role. Here we show that MAP3K1 associates with the cytoskeleton, activates Jun N-terminal kinase (JNK) and actin polymerization, and pro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.001
更新日期:2014-11-01 00:00:00
abstract::The dorsal-ventral axis of the Xenopus embryo is approximately determined by the point of sperm entry, which occurs randomly about the animal-vegetal axis of the egg. Two cytoplasmic components were studied whose spatial arrangements and local concentration patterns appear to reflect the forming dorsal-ventral axis. F...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90457-9
更新日期:1985-06-01 00:00:00
abstract::The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90241-7
更新日期:1987-04-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.05.001
更新日期:2016-07-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.019
更新日期:2003-12-01 00:00:00
abstract::Primitive blood constitutes the ventralmost mesoderm in amphibians, and its cleavage-stage origin reveals important clues about the orientation of the dorsal/ventral axis in the embryo. In recent years, investigators employing various lineage-labeling strategies have reported disparate results for the origin of primit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0717
更新日期:2002-08-01 00:00:00
abstract::During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.06.027
更新日期:2012-09-15 00:00:00
abstract::A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90049-0
更新日期:1983-02-01 00:00:00
abstract::Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.014
更新日期:2009-11-15 00:00:00
abstract::In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme cataly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8999
更新日期:1998-11-15 00:00:00
abstract::The formation of an anterior-posterior (AP) gradient of microtubules in Drosophila oocytes is essential for specification of the AP axis. Proper microtubule organization in the oocyte requires the function of serine/threonine kinase Par-1. The N1S isoform of Par-1 is enriched at the posterior cortex of the oocyte from...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.12.031
更新日期:2009-03-15 00:00:00
abstract::Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.01.010
更新日期:2017-02-15 00:00:00
abstract::MicroRNAs (miRNAs) are post-transcriptional regulators of growth and development in both plants and animals. Flowering is critical for the reproduction of angiosperms. Flower development entails the transition from vegetative growth to reproductive growth, floral organ initiation, and the development of floral organs....
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2013.05.009
更新日期:2013-08-15 00:00:00