Abstract：
:The existence of a high-conductance voltage sensitive chloride channel in BC3H1 myoblasts is documented. The conductance of the channel in symmetrical 150 mmol/l sodium chloride is around 400 pS. The probability of the channel being in the open state decreases with increasing of the imposed voltage from holding potential (0 mV) in both the depolarizing and the hyperpolarizing direction respectively. The bell-shaped open probability plot is asymmetrical, and can be fitted by two Boltzmann equations with different Vh and kn constants; the fitted values were -53 mV and -8.4 respectively for the negative side and +41 mV and +10.2 for the right side. When the unit Cl currents to rectangular pulses are summated the resulting total ionic Cl current shows relaxation, which increases with the amplitude of the pulse. The activation as well as the shape of the current can be significantly influenced by varying the amplitude and the direction of prepulses or holding potential. The high-conductance Cl channel shows several substrates (at least four with amplitudes of around 100 pS); frequently accompanied by flickerings.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Hurnák O,Zachar Jsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
171-82issue
2eissn
0231-5882issn
1338-4325journal_volume
12pub_type
杂志文章abstract：:Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:In the present work have been made some modifications of Münch's model, which concerns the explanation of biophysical bases of the problem of transport of water and assimilates. Then, by using the thermodynamic formalism of Kedem and Katchalsky (1958), we have made a mathematical description of the model. On this basi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Charcot-Marie-Tooth disease (CMT) and related peripheral neuropathies are the most commonly inherited neurological disorders in humans, characterized by clinical and genetic heterogeneity. The most prevalent clinical entities belonging to this group of disorders are CMT type 1A (CMT1A) and hereditary neuropathy with l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_04_379
更新日期：2011-12-01 00:00:00
abstract：:Limited numbers of studies include research of microvibration therapy in experimental models. We examined effects of chronic vibroacustic-microvibration treatment on haemodynamics and anti-oxidative defense in experimental hypertension. Study was performed on chronically treated hypertensive and normotensive Wistar ra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014033
更新日期：2015-01-01 00:00:00
abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:Recent research has supported a potential role of immune pathology in the etiopathogenesis of schizophrenic. In the CNS various viruses were identified in the brains of schizophrenic patients. Pro-inflammatory cytokines were found to be associated with the stage of disease. Microglial cells were reported to be activat...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Although numerous studies have shown the existence of various types of ion conductance in antral part of gastric fundus mucosa epithelia of amphibian, practically no data are available on ion conductance in higher animal species. Present experiments were undertaken to check the possible existence of ion conductance in...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Arterial base deficit/excess (BD/E) is commonly used marker of metabolic acidosis in critically ill patients, but requires an arterial puncture and blood gas analysis. We hypothesized that serum bicarbonate (HCO3), which can be routinely obtained, strongly correlates with arterial BD/E and provides equivalent predicti...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Depression is a brain disorder characterized by severe emotional, cognitive, neuroendocrine and somatic dysfunction. Although the latest generation of antidepressant drugs has improved clinical efficacy and safety, the onset of their clinical effect is significantly delayed after treatment commencement, and a large nu...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2015013
更新日期：2015-10-01 00:00:00
abstract：:Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The effects of different parts of extract from medicinal plant Conyza canadensis, used to control bleeding, on human blood platelet aggregation in vitro were investigated. Aqueous extract of Conyza c. from young or old plants, glycoconjugate part, polysaccharide part and aglycon part at the concentrations above 0.75 m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016039
更新日期：2016-10-01 00:00:00
abstract：:This study investigated the noradrenergic contribution during the cutaneous vasoconstrictor response to local cooling in the leg and forearm. On each limb, one site was perfused with Yoh/Prop to block the postsynaptic adrenoceptors and another with Lactated Ringer's (control) using microdialysis. Blood flow was measur...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017021
更新日期：2018-01-01 00:00:00
abstract：:The hydrolytic activity and interaction of acidic and neutral phospholipase A2 (PLA2) with large unilamellar liposomes treated with cobra venom cytotoxin Vc5 (CT Vc5) were studied to more fully understand the modulating effects of cationic membrane-active peptides on PLA2. Studies were done by fluorescence displacemen...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100, 200 or 400 ng/ml). Following 72-96 h storage at 4ºC, the sperm...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_SI1_36
更新日期：2011-01-01 00:00:00
abstract：:7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020022
更新日期：2020-09-01 00:00:00
abstract：:Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017007
更新日期：2017-10-01 00:00:00
abstract：:The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_005
更新日期：2012-03-01 00:00:00
abstract：:Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb-2019057
更新日期：2020-03-01 00:00:00
abstract：:Beat-to-beat variability of action potential duration (short-term variability, SV) is an intrinsic property of mammalian myocardium. Since the majority of agents and interventions affecting SV may modify also action potential duration (APD), we propose here the concept of relative SV (RSV), where changes in SV are nor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015019
更新日期：2016-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated wi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014023
更新日期：2015-01-01 00:00:00
abstract：:The action of the antiviral drug rimantadine on the structure of bilayer lipid membranes (BLM) and RBC membranes was investigated. Structural changes in BLM were recorded by ionophore conductivity changes and by changes in the third harmonic of capacity current signal due to lateral compression of BLM in an electric f...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:Mitochondrial nucleoids (hereafter nucleoids) contain genetic information, mitochondrial DNA, prerequisite for mitochondrial functioning, particularly information required for mitochondrial electron transport. To understand nucleoid functioning, it is imperative to know its ultrastructure and dynamics in the context o...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016008
更新日期：2016-07-01 00:00:00
