Abstract：
:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in positions 1 and 2: [1-(1-mercapto-4-tert-butylcyclohexaneacetic acid)] AVP (I); [1-(1-mercapto-4-methylcyclohexaneacetic acid)] AVP (II); [1-(1-mercapto-4-methylcyclohexaneacetic acid)-2-O-methyltyrosine] AVP (III); and in position 4: [1-(1-mercaptocyclohexaneacetic acid)-4-arginine] AVP (IV); [1-(2-mercaptopropionic acid)-4-arginine] AVP (V). The addition of synthetic vasopressins I, II and V to the frog skin resulted in a weaker stimulation of the skin sodium transport, measured as the level of the short-circuit current (Isc), as compared to that induced by synthetic AVP. In relation to natriferic activity, analogs III and IV did not change the electrical parameters of the skin. It is concluded that introduction of cyclic structure at the beta-carbon in position 1 of the vasopressin molecule decreased its natriferic activity by about 70%. The same reduction of the activity was caused by the replacement of the glutamine residue in position 4 with arginine, and deamination in position 1. Cyclic structure bound in position 1 together with methylation of tyrosine in position 2 resulted in a full suppression of natriferic activity. Similarly, introduction of cyclic group in position 1 in combination with substitution of glutamine in position 4 with arginine totally abolished natriferic activity.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Bakos P,Ponec J,Alexandrová M,Lichardus B,Lammek B,Rekowski P,Kupryszewski Gsubject
Has Abstractpub_date
1990-12-01 00:00:00pages
605-13issue
6eissn
0231-5882issn
1338-4325journal_volume
9pub_type
杂志文章abstract：:Homocysteine (Hcys) may be implicated in different diseases, especially in cardiovascular illnesses. The most reactive form of Hcys is its cyclic thioester-homocysteine thiolactone (HTL), which is formed in plasma and represents up to 0.29% of plasma total Hcys. Recently, it has been observed that Hcys and HTL may mod...
journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1990-06-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：1989-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
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journal_title：General physiology and biophysics
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更新日期：2001-06-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：2004-09-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1999-12-01 00:00:00
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更新日期：2006-12-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1988-02-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：2003-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1995-10-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：2004-03-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：1986-04-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：2010-03-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：1999-12-01 00:00:00
