Abstract：
:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance also underlies P-gp-positive variants of human acute myeloid leukemia cells (AML) derived from SKM-1 and MOLM-13 cells (SKM-1/VCR, SKM-1/LEN, MOLM-13/VCR) by selection with vincristine (VCR) and lenalidomide (LEN). While SKM-1/LEN cells were P-gp positive, no P-gp was detected in MOLM-13/LEN cells. P-gp-positive cells could be repeatedly passaged in medium containing TNM. In contrast, more than 90% of P-gp-negative cells were entering and progressing through cell death mechanisms after the third passage in medium containing TNM. Combined apoptosis/necrosis cell death was detected in L1210 cells after exposure to TNM. Passaging of P-gp-negative AML cells in medium containing TNM induced preferentially apoptosis. Damage to P-gp-negative cells induced with TNM was associated with arrest in the G1 phase of the cell cycle. P-gp-positive leukemia cells differed from P-gp-negative cells in the composition of plasma membrane glycoproteins, which we monitored with the aid of different lectins. The application of TNM to cells induced additional changes in membrane-linked glycosides.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Pavlikova L,Seres M,Imrichova D,Hano M,Rusnak A,Zamorova M,Katrlik J,Breier A,Sulova Zdoi
10.4149/gpb_2016039subject
Has Abstractpub_date
2016-10-01 00:00:00pages
497-510issue
4eissn
0231-5882issn
1338-4325journal_volume
35pub_type
杂志文章abstract：:Lignin, the structural polymer of the plant cell walls, is produced by free radical polymerization of phenolic alcohols, catalyzed by different peroxidases. The mechanism and the structural organization of lignin in the cell have not been completely understood. In this study we applied fractal analysis to images of li...
journal_title：General physiology and biophysics
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abstract：:The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title：General physiology and biophysics
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abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1986-10-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：1990-08-01 00:00:00
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journal_title：General physiology and biophysics
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更新日期：1990-06-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1991-12-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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