Abstract：
:The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. DNR (3 mg/kg i.v.) + DXZ (60 mg/kg i.p.), and 3. the control group (saline 1 ml/kg i.v. in the same schedule). The drugs were given once weekly, max. 10 administrations. Protein fractions were isolated by stepwise extraction from the samples of the left ventricle. In the DNR-group, the concentrations of both, metabolic and contractile proteins were significantly reduced, while the amount of collagen was significantly higher in comparison with the control group. In the group treated with DNR and DXZ, the concentrations of individual protein fractions (except metabolic proteins) were comparable to those of the control group, which confirms a significant cardioprotective effect of DXZ. The changes of protein profiling corresponded to functional examination of both cardiac parameters (EF, dP/dt(max), PEP: LVET index) and histological examination. These data should be used in further studies dealing with evaluation of cardiotoxic and, possibly, cardioprotective effects of new drugs.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Adamcová M,Pelouch V,Gersl V,Kaplanová J,Mazurová Y,Simůnek T,Klimtová I,Hrdina Rsubject
Has Abstractpub_date
2003-09-01 00:00:00pages
411-9issue
3eissn
0231-5882issn
1338-4325journal_volume
22pub_type
杂志文章abstract：:The human pituitary tumor-transforming gene-1 (hPTTG1) has been found to be overexpressed in various cancers. Accumulated evidences implicate that some of protein kinases can specifically recognize two PXXP motifs at hPTTG1 C-terminus through their Src homology (SH3) domain and then phosphorylate the protein by their ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016035
更新日期：2017-01-01 00:00:00
abstract：:Culturing the fresh-water alga Hydrodictyon reticulatum for 9 days in the presence of 0.2 and 0.4 mmol/l Ba2+ increases the membrane potential about twice and the plasmalemma resistance 5-10 times without having any significant effect on the cell contents of water and Na+, K+ and Cl- ions. While in control cells the m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Cochlear spiral ganglion neurons provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Serotonin plays a role in the response properties of central auditory neurons. However, knowledge about the role of serotonin in the peripheral auditory nervous system re...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013005
更新日期：2013-03-01 00:00:00
abstract：:The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018017
更新日期：2018-09-01 00:00:00
abstract：:Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_04_373
更新日期：2010-12-01 00:00:00
abstract：:In the present study effects of a new local anaesthetics, pentacaine (trans-2-pyrolidinocyclohexylester of 3-pentyloxyphenylcarbamic acid), and of some chemically related compounds on rat hepatic adenylate cyclase activity were studied under various experimental conditions. As compared with tetracaine, the local anaes...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:This study was designed to investigate the possible effectiveness of chronic resveratrol administration on redox state, inflammatory mediators and apoptosis rate in diabetic rats. Male Wistar rats were divided into four groups (n = 6): normal control, diabetic control, normal rats treated with resveratrol, and diabeti...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_039
更新日期：2012-12-01 00:00:00
abstract：:Diabetes mellitus leads to bone disorders such as osteopenia and osteoporosis that can increase fracture risk. On the other hand, sodium tungstate is an inorganic compound which exerts anti-diabetic activity in experimental studies due to its suggested insulin-mimetic or antioxidant activity. Therefore this study was ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014020
更新日期：2014-01-01 00:00:00
abstract：:Voltage-activated calcium channels can be divided into two subgroups based on their activation threshold, low-voltage-activated (LVA) and high-voltage-activated (HVA). Auxiliary subunits of the HVA calcium channels contribute significantly to biophysical properties of the channels. We have cloned and characterized mem...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2005-06-01 00:00:00
abstract：:Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Aquaporin attracted attention not only of physiologists and biophysicists, but also of chemical engineers. Here we critically analyze a paper describing aquaporin-based artificial membranes, suggested for forward osmosis-based water purification (Wang et al. 2012, Small 8, pp. 1185-1190). Related papers published late...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
abstract：:Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Low-conductance chloride channel from skeletal muscle SR vesicles of the crayfish Astacus fluviatilis was incorporated into planar lipid bilayers and its basic characteristics were investigated. The channel has a relatively low unitary conductance of 26 pS in symmetrical 160 mmol/l choline-chloride. The dependence of ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Previously detected βsp and γ1sp dielectric relaxations on the spectrin-based membrane skeleton (MS) of human red blood cells (RBCs) have been shown sensitive to the attachment of MS to the lipid-protein membrane. Such relaxations were now detected on the MS of mammal (rat, horse, bovine, sheep and goat) and "unstrain...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020034
更新日期：2020-11-01 00:00:00
abstract：:For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_03_266
更新日期：2010-09-01 00:00:00
abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Gastric cancer (GC) is a high mortality disease. We studied the function and mechanism of long non-coding RNA prostate cancer-associated transcript 6 (lncRNA PCAT6) on cell proliferation and epithelial-mesenchymal transition (EMT) in GC cells. CCK-8, flow cytometry and colony formation assay were respectively used to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019044
更新日期：2020-01-01 00:00:00
abstract：:Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014043
更新日期：2015-07-01 00:00:00
abstract：:Hyperglycemia impairs oxidative capacity in skeletal muscle. Muscle oxidative capacity is regulated by peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α). Transcutaneous carbon dioxide (CO2) enhances PGC-1α expression in skeletal muscle. Therefore, the aim of this study was to clarify the effects of...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018048
更新日期：2019-05-01 00:00:00
abstract：:The effects of both chronic hypoxia and acute intermittent hypoxia (AIH) on cardiovascular system are unclear. We designed this study to develop a rabbit model of hypertension by exposure to chronic hypobaric hypoxia (CHH) and to investigate the effects of AIH on hypertensive rabbits. Present study was performed in 13...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013070
更新日期：2014-01-01 00:00:00
abstract：:It is well known that in vitro storage lesions lead to membrane dysfunction and decreased number of functional erythrocytes. As erythrocytes get older, in storage media as well as in peripheral circulation, they undergo a variety of biochemical changes. In our study, the erythrocytes with different age groups in citra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016001
更新日期：2016-07-01 00:00:00
abstract：:In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potentia...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016057
更新日期：2017-07-01 00:00:00
abstract：:Apoptosis (programmed cell death) is an important process participating in the formation of organs and tissues during embryogenesis. Our aim of the work is studying the role of the apoptosis during the human embryonic differentiation. We tend to give acquired findings into the correlation with expression of proteins B...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
abstract：:Depression is a brain disorder characterized by severe emotional, cognitive, neuroendocrine and somatic dysfunction. Although the latest generation of antidepressant drugs has improved clinical efficacy and safety, the onset of their clinical effect is significantly delayed after treatment commencement, and a large nu...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2015013
更新日期：2015-10-01 00:00:00
abstract：:The distributions of K, Na, Mg and Ca within frog ovarian and oviductal oocytes were studied by electron probe wavelength dispersive X-ray microanalysis. An important heterogeneity could be found both in nuclear and jelly coated oocytes. The highest K, Mg and, to a lesser extent, Na concentrations were found in the pi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:In this study, we investigated the role of AMP-activated protein kinase (AMPK) and the importance of reactive oxygen species (ROS) in apoptosis induction associated with cell cycle arrest induced by sulforaphane in AGS human gastric cancer cells. Our results demonstrated that sulforaphane inhibited proliferation of AG...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017026
更新日期：2018-03-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Semiserial sections of lung capillaries of seven rabbits were examined for megakaryocytes. Triads of serial sections of each lobe were examined, and megakaryocyte counts per cm2 were determined. Median megakaryocyte count was determined for each lobe. The lobal values were used to calculate the was determined for each...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
