Abstract：
:Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH and to validate such exposure by real-time O(2) measurements and cellular responses. Standard 24-well culture plates and plates with bottoms made from a gas permeable film were placed in a heated cabinet. Desired cycling of O(2) levels was induced using programmable solenoids to purge mixtures of 95% N(2) + 5% CO(2) or 95% O(2) + 5% CO(2). Dissolved oxygen, gas pressure, temperature, and water evaporation were measured during cycling. IH-induced cellular effects were evaluated by hypoxia inducible factor (HIF) and NF-κB luciferase reporters in HEK296 cells and by insulin secretion in rat insulinoma cells. Oxygen cycling in the cabinet was translated into identical changes of O(2) at the well bottom in gas permeable, but not in standard cultureware. Twenty-four hours of IH exposure increased HIF (112%), NF-κB (111%) and insulin secretion (44%). Described system enables reproducible and prolonged IH exposure in cultured cells while controlling for important environmental factors.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Polak J,Studer-Rabeler K,McHugh H,Hussain MA,Shimoda LAdoi
10.4149/gpb_2014043subject
Has Abstractpub_date
2015-07-01 00:00:00pages
235-47issue
3eissn
0231-5882issn
1338-4325journal_volume
34pub_type
杂志文章abstract：:The membrane lipid and fatty acid compositions of red blood cells from a paramyotonia patient were investigated. Cholesterol and total phospholipid contents in paramyotonia were not different from control. Only the sphingomyelin content was lower, and thus the molar ratio of phosphatidylcholine/sphingomyelin was highe...
journal_title：General physiology and biophysics
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更新日期：1986-04-01 00:00:00
abstract：:Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats recei...
journal_title：General physiology and biophysics
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abstract：:A mechanical model of heart muscle is proposed which includes rheological equations and equations for Ca-troponin interaction, for the dependences of the number of myosin cross-bridges on the length of sarcomere and on the speed of motion. The main assumption of the model is the dependence of the troponin affinity to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The action of the antiviral drug rimantadine on the structure of bilayer lipid membranes (BLM) and RBC membranes was investigated. Structural changes in BLM were recorded by ionophore conductivity changes and by changes in the third harmonic of capacity current signal due to lateral compression of BLM in an electric f...
journal_title：General physiology and biophysics
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更新日期：1989-02-01 00:00:00
abstract：:A model of activation of muscle contraction has been proposed. It is based on calcium diffusion and binding to specific regulatory sites in a sarcomere. Calcium ions activate interactions of contractile proteins and thus the generation of force. The model quantifies the relation between calcium released from intracell...
journal_title：General physiology and biophysics
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doi：
更新日期：1989-04-01 00:00:00
abstract：:Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
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doi：10.4149/gpb_2010_04_373
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journal_title：General physiology and biophysics
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更新日期：2000-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
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更新日期：1998-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1994-02-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1996-08-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1999-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_02_140
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017006
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019032
更新日期：2019-09-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1986-08-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：1994-04-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：2010-03-01 00:00:00
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journal_title：General physiology and biophysics
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doi：10.4149/gpb_2019019
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017020
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abstract：:The hydrolytic activity and interaction of acidic and neutral phospholipase A2 (PLA2) with large unilamellar liposomes treated with cobra venom cytotoxin Vc5 (CT Vc5) were studied to more fully understand the modulating effects of cationic membrane-active peptides on PLA2. Studies were done by fluorescence displacemen...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1991-06-01 00:00:00
abstract：:The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
