Abstract：
:A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxation phase respectively) with subsequent calculation of the maximal amplitude--normalized relaxation rate, Vn. The use of the method is illustrated on ureter, protal vein, vas deferens, myometrium of rat and guinea-pig ureter smooth muscles in a variety of experimental conditions. This method can be applied for the description of the calcium transient decay in the myoplasm measured with Ca-sensitive fluorescent dyes. The method might be useful for identification of the contribution of different energy-dependent Ca-transporting systems involved in the control of relaxation, as well as for screening of the mechanisms of action of different factors which modulate the contraction-relaxation cycle.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Burdyga V,Kosterin SAsubject
Has Abstractpub_date
1991-12-01 00:00:00pages
589-98issue
6eissn
0231-5882issn
1338-4325journal_volume
10pub_type
杂志文章abstract：:Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:The aim of present study was to examine the impact of prenatal ethanol exposure on seizure susceptibility of the offspring. Pregnant Wistar rats were compelled to drink either 10% or 20% ethanol solution, as the only drinking fluid since conception up to the weaning of their offspring at the age of 28 days. Pregnant a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_019
更新日期：2012-06-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Hyperglycemia impairs oxidative capacity in skeletal muscle. Muscle oxidative capacity is regulated by peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α). Transcutaneous carbon dioxide (CO2) enhances PGC-1α expression in skeletal muscle. Therefore, the aim of this study was to clarify the effects of...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018048
更新日期：2019-05-01 00:00:00
abstract：:Recent findings suggest that polymorphisms in vitamin D pathway genes are candidates for association with multiple sclerosis susceptibility. It has been now well demonstrated that vitamin D has immunomodulatory functions that may be favorable for reduction of multiple sclerosis risk. Current research has been focused ...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2013067
更新日期：2013-12-01 00:00:00
abstract：:The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. D...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Activated endothelial cells play a crucial role in the formation of new blood vessels, a process known as angiogenesis, which can underlie the development of several diseases. Different antiangiogenic therapies aimed against vascular endothelial growth factor (VEGF), the dominant pro-angiogenic cytokine, have been dev...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018047
更新日期：2019-03-01 00:00:00
abstract：:Results of studies on the modulation of skeletal muscle contraction by phosphorylation of myosin regulatory light chains and by exchange of magnesium for calcium in myosin heads were reviewed. The polarized fluorescence method was used in these studies, and conformational changes of contractile proteins accompanying m...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1991-06-01 00:00:00
abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:The human pituitary tumor-transforming gene-1 (hPTTG1) has been found to be overexpressed in various cancers. Accumulated evidences implicate that some of protein kinases can specifically recognize two PXXP motifs at hPTTG1 C-terminus through their Src homology (SH3) domain and then phosphorylate the protein by their ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016035
更新日期：2017-01-01 00:00:00
abstract：:Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_005
更新日期：2012-03-01 00:00:00
abstract：:Homocysteine (Hcys) may be implicated in different diseases, especially in cardiovascular illnesses. The most reactive form of Hcys is its cyclic thioester-homocysteine thiolactone (HTL), which is formed in plasma and represents up to 0.29% of plasma total Hcys. Recently, it has been observed that Hcys and HTL may mod...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_02_186
更新日期：2010-06-01 00:00:00
abstract：:In the present study effects of a new local anaesthetics, pentacaine (trans-2-pyrolidinocyclohexylester of 3-pentyloxyphenylcarbamic acid), and of some chemically related compounds on rat hepatic adenylate cyclase activity were studied under various experimental conditions. As compared with tetracaine, the local anaes...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:The absorption of glucose (free, and released from membrane hydrolysis of maltose) and water in the isolated loop of the rat small intestine was studied in chronic experiments. Even at maximum glucose (75 mmol/l) or maltose (37.5 mmol/l) concentrations the rate of glucose transfer by solvent drag and by diffusion did ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Diabetes mellitus leads to bone disorders such as osteopenia and osteoporosis that can increase fracture risk. On the other hand, sodium tungstate is an inorganic compound which exerts anti-diabetic activity in experimental studies due to its suggested insulin-mimetic or antioxidant activity. Therefore this study was ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014020
更新日期：2014-01-01 00:00:00
abstract：:Lidocaine, a use-dependent Na(+) channel blocker, paradoxically evokes neural activation in the slowly adapting stretch receptor organ of crayfish at 5-10 mmol/l concentration. For elucidating the underlying mechanisms of this paradoxical effect, a series of conventional electrophysiological experiments were performed...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix[6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013024
更新日期：2013-06-01 00:00:00
abstract：:Changes in the Young elasticity modulus in perpendicular direction to the membrane surface E perpendicular, in the coefficient of dynamic viscosity eta, in the electric capacitance C, in the surface charge U1, in the conductivity g and in the coefficient of non-linearity beta of current-voltage characteristic caused b...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Depression is a brain disorder characterized by severe emotional, cognitive, neuroendocrine and somatic dysfunction. Although the latest generation of antidepressant drugs has improved clinical efficacy and safety, the onset of their clinical effect is significantly delayed after treatment commencement, and a large nu...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2015013
更新日期：2015-10-01 00:00:00
abstract：:The effect of membrane composition on ethanol partitioning into lipid bilayers was assessed by headspace gas chromatography. A series of model membranes with different compositions have been investigated. Membranes were exposed to a physiological ethanol concentration of 20 mmol/l. The concentration of membranes was 2...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_02_140
更新日期：2009-06-01 00:00:00
abstract：:The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The effects of pentoxifylline (PTX) on intracellular accumulation of doxorobicin (DOX), DOX cytotoxicity and expression of Pgp in multidrug resistant L1210/VCR cell line were investigated. PTX (100 mg/l) was able to enhance the DOX accumulation in resistant cells. The maximum intracellular levels of DOX were reached a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Many herbicides employed in agriculture and also some antibiotics bind to a specific site of the reaction centre protein (RC) blocking the photosynthetic electron transport. Crystal structures showed that all these compounds bind at the secondary ubiquinone (QB) site albeit to slightly different places. Different herb...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015003
更新日期：2015-04-01 00:00:00
abstract：:The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100, 200 or 400 ng/ml). Following 72-96 h storage at 4ºC, the sperm...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_SI1_36
更新日期：2011-01-01 00:00:00
abstract：:Effects of haloperidol on L-type CaV1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV1.2a) and vascular (CaV1.2b) splice...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_03_249
更新日期：2009-09-01 00:00:00
abstract：:In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
