Abstract：
:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between the CD26 antigen expression and DPP IV positivity in the vast majority of T lymphoblasts in T-ALL patients was observed. No CD26 was expressed on DPP IV negative T cells. The variable CD4 and/or CD8 antigen expression, frequent CD7 positivity and absence of membrane CD3 antigen expression were the characteristic immunophenotypic features of CD26/DPP IV positive T cells. CD26/DPP IV activity strongly paralleled the CD71 antigen (transferrin receptor, T cell activation/proliferation antigen) expression. The phenotypic features of CD26/DPP IV positive T cells are characteristic for the relative immature cell population. Noteworthy was the slight disassociation between the very high CD26 antigen J expression and moderate DPP IV activity in cells of some T-ALL patients. The possible existence of enzymatically inactive structures of CD26 antigen or inactive precursors of DPP IV detectable only by immunocytochemistry was discussed. Our study indicates that CD26 antigen expression is tended to identify cells with DPP IV enzymatic activity in T-ALL patients. The results provide information of CD26 antigen possible involvement in the pathology of leukemic cells via its DPP IV enzyme activity.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Klobusická M,Babusíková Osubject
Has Abstractpub_date
1999-12-01 00:00:00pages
34-7eissn
0231-5882issn
1338-4325journal_volume
18 Suppl 1pub_type
杂志文章abstract：:Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_04_373
更新日期：2010-12-01 00:00:00
abstract：:Cochlear spiral ganglion neurons provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Serotonin plays a role in the response properties of central auditory neurons. However, knowledge about the role of serotonin in the peripheral auditory nervous system re...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013005
更新日期：2013-03-01 00:00:00
abstract：:The relationship between erythrocyte shape and the critical cell volume was investigated. Agents able to increase the critical cell volume induced three main stable shapes of erythrocytes: discocytic, stomatocytic, and echinocytic. The absence of correlation between shape and critical cell volume under isoosmotic cond...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
abstract：:Kinase-targeted therapy is a new and promising approach to disease treatment. However, some kinase inhibitors have been observed to cause an off-target adverse risk for skeletal system by influencing the growth of osteoblasts. It is known that the proliferation and differentiation of osteoblasts are essentially regula...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018030
更新日期：2019-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016033
更新日期：2017-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_02_154
更新日期：2011-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015018
更新日期：2016-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:Mitochondrial nucleoids (hereafter nucleoids) contain genetic information, mitochondrial DNA, prerequisite for mitochondrial functioning, particularly information required for mitochondrial electron transport. To understand nucleoid functioning, it is imperative to know its ultrastructure and dynamics in the context o...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016008
更新日期：2016-07-01 00:00:00
abstract：:The existence of a high-conductance voltage sensitive chloride channel in BC3H1 myoblasts is documented. The conductance of the channel in symmetrical 150 mmol/l sodium chloride is around 400 pS. The probability of the channel being in the open state decreases with increasing of the imposed voltage from holding potent...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Cytochrome P-450 and NADPH: cytochrome P-450 reductase were solubilized by detergents from microsomal fraction of tulip bulbs Tulipa fosteriana L. and purified to electrophoretic homogeneity. The purification was achieved by anion-exchange column chromatography, hydroxyapatite-column chromatography and affinity chroma...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix[6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013024
更新日期：2013-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:Homeostasis and development in vertebrates are regulated by cell proliferation, differentiation and death. Permeability of mitochondrial membranes, a decisive feature of apoptosis, is regulated by Bcl-2 family regulators. Protein p53 is able to reduce bcl-2 and promote bax expression. This study focused on the immunoh...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:S100A1 is a Ca2+-binding protein expressed at high levels in the myocardium. It is thought to modulate the Ca2+ sensitivity of the sarcoplasmic reticulum (SR) Ca2+ release channels (ryanodine receptors or RyRs) and its expression has been shown to be down regulated in various heart diseases. In this study we used S100...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_04_371
更新日期：2009-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated wi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014023
更新日期：2015-01-01 00:00:00
abstract：:This study was designed to investigate the possible effectiveness of chronic resveratrol administration on redox state, inflammatory mediators and apoptosis rate in diabetic rats. Male Wistar rats were divided into four groups (n = 6): normal control, diabetic control, normal rats treated with resveratrol, and diabeti...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_039
更新日期：2012-12-01 00:00:00
abstract：:Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Recent research has supported a potential role of immune pathology in the etiopathogenesis of schizophrenic. In the CNS various viruses were identified in the brains of schizophrenic patients. Pro-inflammatory cytokines were found to be associated with the stage of disease. Microglial cells were reported to be activat...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Results of studies on the modulation of skeletal muscle contraction by phosphorylation of myosin regulatory light chains and by exchange of magnesium for calcium in myosin heads were reviewed. The polarized fluorescence method was used in these studies, and conformational changes of contractile proteins accompanying m...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1991-06-01 00:00:00
abstract：:Hyperlipidemia is found to be associated with changes in fatty acid (FA) profiles. The aim of this study was to investigate the effects of AHA-Step-1 dietary treatment and combination of fibrates (gemfibrozil) with dietary intervention on serum and erythrocyte phospholipid FA composition in human hyperlipidemia. 78 st...
journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:A number of computational methods for the description of the ligand-receptor interaction that were developed in the past decade are reviewed in this paper. The central two sections introduce the methods that are already established as useful tools for the qualitative and quantitative description of ligand-receptor com...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1999-09-01 00:00:00
