Abstract:
:The absorption of glucose (free, and released from membrane hydrolysis of maltose) and water in the isolated loop of the rat small intestine was studied in chronic experiments. Even at maximum glucose (75 mmol/l) or maltose (37.5 mmol/l) concentrations the rate of glucose transfer by solvent drag and by diffusion did not exceed 13% and 25%, respectively, of the total rate of glucose absorption. Electron microscopic and immunocytochemical analysis revealed a significant widening of intercellular gaps in the basal epithelium region and an increase of actin density in the vicinity of the tight junctions and between the apical root filaments in enterocytes after glucose or glycine load in chronic and acute experiments. However, very rarely (in 1% of all cases), and only in chronic experiments, structural changes in the tight junctions such as "blisters" and dilatations were also recognised. It is concluded that under normal physiological conditions the absorption of glucose (free, and released from maltose hydrolysis) mainly uses active transport across the apical membrane of the enterocytes.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Ugolev AM,Komissarchik YaYu,Gromova LV,Gruzdkov AA,Snigirevskaya ES,Brudnaya MSsubject
Has Abstractpub_date
1995-10-01 00:00:00pages
405-17issue
5eissn
0231-5882issn
1338-4325journal_volume
14pub_type
杂志文章abstract::The present results suggest that laminin-1 and 3 are localized in the specialized Schwann cells of Pacinian corpuscles, in spite of incomplete deposition of the basal lamina on the surface of their cytoplasmic processes. In addition, laminin-3 is concentrated and probably function as a stop protein not only in the neu...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013077
更新日期:2014-01-01 00:00:00
abstract::Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::In the present study effects of a new local anaesthetics, pentacaine (trans-2-pyrolidinocyclohexylester of 3-pentyloxyphenylcarbamic acid), and of some chemically related compounds on rat hepatic adenylate cyclase activity were studied under various experimental conditions. As compared with tetracaine, the local anaes...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2012_005
更新日期:2012-03-01 00:00:00
abstract::The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2010_03_266
更新日期:2010-09-01 00:00:00
abstract::The activity of different cathepsins and neutral proteinases was measured in normal and vitamin E-deficient rabbit muscles using specific substrates. Among the changes of enzyme activities in dystrophy caused by vitamin E-deficiency the increase in the activity of cathepsin B is the most striking. The activity of cath...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BamHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the p...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::A number of studies have reported that male reproductive organs are susceptible to electromagnetic fields (EMFs). The aim of this study was to explore the effects of microwave radiation exposures on 6-8 weeks old male Swiss albino mice. Mice were divided into two groups: group I - sham-exposed (Control), and group II ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017059
更新日期:2018-07-01 00:00:00
abstract::The aim of the present work was to test the sensitivity of young male Wistar rats, Dobrá Voda (Dv:WI) to the diabetogenic effect of streptozotocin (STZ) with regard to their health condition and mortality rates. Eight-week-old rats, weighing from 200 to 230 g, were randomised into five groups of eight animals. Strepto...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphism...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017033
更新日期:2017-12-01 00:00:00
abstract::The effects of different parts of extract from medicinal plant Conyza canadensis, used to control bleeding, on human blood platelet aggregation in vitro were investigated. Aqueous extract of Conyza c. from young or old plants, glycoconjugate part, polysaccharide part and aglycon part at the concentrations above 0.75 m...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::The passive mechanical properties of whole muscle in active and nonactive states are compared. The experimental results are presented as stress-strain curves, which are analyzed in the framework of the current theoretical background [viz. the freely-jointed chain model (FJCM) and the worm-like chain model (WLCM)] in a...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Results of studies on the modulation of skeletal muscle contraction by phosphorylation of myosin regulatory light chains and by exchange of magnesium for calcium in myosin heads were reviewed. The polarized fluorescence method was used in these studies, and conformational changes of contractile proteins accompanying m...
journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:1991-06-01 00:00:00
abstract::The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. D...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::The relationship between erythrocyte shape and the critical cell volume was investigated. Agents able to increase the critical cell volume induced three main stable shapes of erythrocytes: discocytic, stomatocytic, and echinocytic. The absence of correlation between shape and critical cell volume under isoosmotic cond...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018017
更新日期:2018-09-01 00:00:00
abstract::Diabetic cardiomyopathy is a severe complication of diabetes mellitus (DM). The goal of current work was to study the effects of sinomenine on streptozotocin-induced cardiomyopathy in rats. DM in rats was induced by intraperitoneal injection of streptozotocin. Cardiac function was evaluated by measuring left ventricle...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016033
更新日期:2017-01-01 00:00:00
abstract::Although the A1 adenosine receptor (A1 receptor), the main adenosine receptor type in cardiac muscle, is involved in powerful cardioprotective processes such as ischemic preconditioning, the atrial A1 receptor reserve has not yet been quantified for the direct negative inotropic effect of adenosine. In the present stu...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013041
更新日期:2013-09-01 00:00:00
abstract::Mitochondrial nucleoids (hereafter nucleoids) contain genetic information, mitochondrial DNA, prerequisite for mitochondrial functioning, particularly information required for mitochondrial electron transport. To understand nucleoid functioning, it is imperative to know its ultrastructure and dynamics in the context o...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016008
更新日期:2016-07-01 00:00:00
abstract::In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016039
更新日期:2016-10-01 00:00:00
abstract::The aim of present study was to examine the impact of prenatal ethanol exposure on seizure susceptibility of the offspring. Pregnant Wistar rats were compelled to drink either 10% or 20% ethanol solution, as the only drinking fluid since conception up to the weaning of their offspring at the age of 28 days. Pregnant a...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2012_019
更新日期:2012-06-01 00:00:00
abstract::The mechanisms underlying the renoprotective effect for remote limb ischemic preconditioning (rIPC) against renal ischemia/reperfusion injury need further elucidation. In our work, one hundred and twenty male Sprague Dawley rats were randomized into 3 groups; sham, I/R group (left renal 45 min ischemia) and rIPC (as I...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015035
更新日期:2016-01-01 00:00:00
abstract::The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old a...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017020
更新日期:2018-03-01 00:00:00