Abstract：
:The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully adapted to multichannel bilayers to estimate the radius of the larger opening of the low conductance state of the channel induced by Staphylococcus aureus alpha-toxin. At the pH used, the channel transition to a low conductance state was accompanied by a decrease of the opening radius from 1.3 +/- 0.2 nm to 0.9 +/- 0.1 nm. The determination criteria for maximum size of a channel opening when using the non-electrolyte exclusion method is discussed.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Krasilnikov OV,Merzlyak PG,Yuldasheva LN,Nogueira RAsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
349-63issue
4eissn
0231-5882issn
1338-4325journal_volume
17pub_type
杂志文章abstract：:Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. D...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:Changes in the Young elasticity modulus in perpendicular direction to the membrane surface E perpendicular, in the coefficient of dynamic viscosity eta, in the electric capacitance C, in the surface charge U1, in the conductivity g and in the coefficient of non-linearity beta of current-voltage characteristic caused b...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_04_373
更新日期：2010-12-01 00:00:00
abstract：:Beat-to-beat variability of action potential duration (short-term variability, SV) is an intrinsic property of mammalian myocardium. Since the majority of agents and interventions affecting SV may modify also action potential duration (APD), we propose here the concept of relative SV (RSV), where changes in SV are nor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015019
更新日期：2016-01-01 00:00:00
abstract：:Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the abil...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018017
更新日期：2018-09-01 00:00:00
abstract：:Although the A1 adenosine receptor (A1 receptor), the main adenosine receptor type in cardiac muscle, is involved in powerful cardioprotective processes such as ischemic preconditioning, the atrial A1 receptor reserve has not yet been quantified for the direct negative inotropic effect of adenosine. In the present stu...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013041
更新日期：2013-09-01 00:00:00
abstract：:A patient with a strongly lesioned spinal cord, sub C5, relearned running, besides improving other movements, by an oscillator formation training (rhythmic, dynamic, stereotyped exercise). After 45 days of jumping on a springboard and other rhythm trainings, the patient was able to run 90 m in 41 s (7.9 km/h) (even 9....
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Maternal milk is a significant source of hormones and other bioactive substances. They might be involved either in the control of mammary gland function or in the regulation of growth and development of the neonate. Atriopeptin (atrial natriuretic factor, ANF) is a peptide with strong diuretic, natriuretic and vasorel...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:We studied the involvement of O2, pH and low molecular thiols in H2S-induced decomposition of S-nitrosoglutathione (GSNO). The GSNO decomposition - •NO release was evaluated by UV-VIS spectroscopy and Griess assay. The H2S donor Na2S was used. O2 slightly increased, but was not necessary for the H2S-induced GSNO decom...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013026
更新日期：2013-09-01 00:00:00
abstract：:2-3-dimercapto-1-propane sulfonic acid, D-penicillamine and meso-dimercapto succinic acid, drugs widely applied as antidota against metal poisoning, and cysteine and glutathione were studied with respect to their ability to generate and to scavenge superoxide anion radical. Superoxide production and scavenging were te...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Calcium ions mediate extracellular signals on intracellular processes. The signalling system based on transient rises or oscillations of the cytoplasmic calcium concentration has potential advantages. The relevant mechanisms of intracellular concentration changes include calcium-induced calcium release and calcium dep...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Culturing the fresh-water alga Hydrodictyon reticulatum for 9 days in the presence of 0.2 and 0.4 mmol/l Ba2+ increases the membrane potential about twice and the plasmalemma resistance 5-10 times without having any significant effect on the cell contents of water and Na+, K+ and Cl- ions. While in control cells the m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Overconsumption of fructose increases the risk of nonalcoholic fatty liver disease (NAFLD), obesity and metabolic syndrome. NAFLD is currently one of the most common etiologies of chronic liver disease worldwide. The aim of the present study is to evaluate the hepatoprotective potential of zingerone against fructose-e...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015041
更新日期：2016-04-01 00:00:00
abstract：:Nucleosome sliding and nucleosome digestion are two main ways for regulating gene transcription. We constructed three characteristic parameters (CP) based on the information of CG0, CG1 and CG2 motifs, and used these parameters to analyze the sliding trend of -1 and +1 nucleosomes around TSS of genes with NFR in yeast...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020002
更新日期：2020-05-01 00:00:00
abstract：:The hormone leptin, by binding to hypothalamic receptors, suppresses food intake and decreases body adiposity. Leptin receptors are also widely expressed in extra-hypothalamic areas such as hippocampus, amygdala and cerebellum, where leptin modulates synaptic transmission. Here we show that a defective leptin receptor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013056
更新日期：2014-01-01 00:00:00
abstract：:Liposomes and proteoliposomes obtained from rat brain were used; structural changes induced by Vc5 cytotoxin (CT) from Central Asia cobra venom have been studied by the EPR method using spin probes (5-, 10-, or 12-doxylstearic acid). The addition of CT to liposome samples, containing spin probes resulted in the appear...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_03_266
更新日期：2010-09-01 00:00:00
abstract：:The distributions of K, Na, Mg and Ca within frog ovarian and oviductal oocytes were studied by electron probe wavelength dispersive X-ray microanalysis. An important heterogeneity could be found both in nuclear and jelly coated oocytes. The highest K, Mg and, to a lesser extent, Na concentrations were found in the pi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:In the present work have been made some modifications of Münch's model, which concerns the explanation of biophysical bases of the problem of transport of water and assimilates. Then, by using the thermodynamic formalism of Kedem and Katchalsky (1958), we have made a mathematical description of the model. On this basi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The effect of melatonin on reperfusion arrhythmias and postischemic contractile dysfunction was studied in the isolated rat heart. 25 min global ischemia was induced and followed by 30 min of reperfusion. Melatonin (10 micromol/l) was present in the perfusion solution during the whole experiment. Experiment revealed p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Diabetic cardiomyopathy is a severe complication of diabetes mellitus (DM). The goal of current work was to study the effects of sinomenine on streptozotocin-induced cardiomyopathy in rats. DM in rats was induced by intraperitoneal injection of streptozotocin. Cardiac function was evaluated by measuring left ventricle...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016033
更新日期：2017-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated wi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014023
更新日期：2015-01-01 00:00:00
