Abstract：
:Although the A1 adenosine receptor (A1 receptor), the main adenosine receptor type in cardiac muscle, is involved in powerful cardioprotective processes such as ischemic preconditioning, the atrial A1 receptor reserve has not yet been quantified for the direct negative inotropic effect of adenosine. In the present study, adenosine concentration-effect (E/c) curves were constructed before and after pretreatment with FSCPX (8-cyclopentyl-N3-[3-(4-(fluorosulfonyl)benzoyloxy)propyl]-N1-propylxanthine), an irreversible A1 receptor antagonist, in isolated guinea pig atria. To prevent the intracellular elimination of the administered adenosine, NBTI (S-(2-hydroxy-5-nitrobenzyl)-6-thioinosine), a nucleoside transport inhibitor, was used. As expected, NBTI alone and FSCPX-pretreatment alone shifted the adenosine E/c curve to the left and right, respectively. However, in the presence of NBTI, FSCPX-pretreatment appeared to increase the maximal response to adenosine. By means of the receptorial responsiveness method (RRM), our recently developed procedure, adenosine E/c curves generated in the presence of NBTI were corrected for the bias caused by the endogenous adenosine accumulated by NBTI. The corrected curves indicate a substantial A1 receptor reserve for the direct negative inotropy evoked by adenosine. In addition, our results suggest that accumulation of an endogenous agonist may bias the E/c curve constructed with the same or similar agonist that can lead to seemingly paradoxical results.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Kiss Z,Pak K,Zsuga J,Juhasz B,Varga B,Szentmiklosi AJ,Haines DD,Tosaki A,Gesztelyi Rdoi
10.4149/gpb_2013041subject
Has Abstractpub_date
2013-09-01 00:00:00pages
325-35issue
3eissn
0231-5882issn
1338-4325journal_volume
32pub_type
杂志文章abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Culturing the fresh-water alga Hydrodictyon reticulatum for 9 days in the presence of 0.2 and 0.4 mmol/l Ba2+ increases the membrane potential about twice and the plasmalemma resistance 5-10 times without having any significant effect on the cell contents of water and Na+, K+ and Cl- ions. While in control cells the m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019032
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013026
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
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doi：10.4149/gpb_2017006
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journal_title：General physiology and biophysics
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doi：
更新日期：2005-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. Cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of Helix pomatia (diameter: 150 microns) served as the test cells. The l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:A new theory termed "tunnel-acid-group-potential" (TAGPT), explaining the effect of pHo and pH(i) on ion conductance through different membrane channels, is presented. It is suggested that shifts in pHo and pH(i) lead to changes in values of negative charges generated by acid groups of side chains of some polar (Glu-,...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016039
更新日期：2016-10-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017021
更新日期：2018-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_03_301
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Results of studies on the modulation of skeletal muscle contraction by phosphorylation of myosin regulatory light chains and by exchange of magnesium for calcium in myosin heads were reviewed. The polarized fluorescence method was used in these studies, and conformational changes of contractile proteins accompanying m...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1991-06-01 00:00:00
abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...
journal_title：General physiology and biophysics
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doi：
更新日期：2000-12-01 00:00:00
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journal_title：General physiology and biophysics
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doi：
更新日期：2004-06-01 00:00:00
abstract：:Apoptosis (programmed cell death) is an important process participating in the formation of organs and tissues during embryogenesis. Our aim of the work is studying the role of the apoptosis during the human embryonic differentiation. We tend to give acquired findings into the correlation with expression of proteins B...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Calcium ions mediate extracellular signals on intracellular processes. The signalling system based on transient rises or oscillations of the cytoplasmic calcium concentration has potential advantages. The relevant mechanisms of intracellular concentration changes include calcium-induced calcium release and calcium dep...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Malate dehydrogenases (MDH, L-malate:NAD oxidoreductase, EC 1.1.1.37), catalyze the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate/aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondria...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2002-09-01 00:00:00
abstract：:In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
abstract：:This study specified the role of several key calcium-operating ion channels in contraction/relaxation of human detrusor muscle as possible target for overactive bladder (OAB) treatment. Detrusor samples, obtained from 18 males (average age 61.5 ± 5.9 years), were investigated by organ tissue bath method with following...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017064
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abstract：:Hyperlipidemia is found to be associated with changes in fatty acid (FA) profiles. The aim of this study was to investigate the effects of AHA-Step-1 dietary treatment and combination of fibrates (gemfibrozil) with dietary intervention on serum and erythrocyte phospholipid FA composition in human hyperlipidemia. 78 st...
journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Nucleosome sliding and nucleosome digestion are two main ways for regulating gene transcription. We constructed three characteristic parameters (CP) based on the information of CG0, CG1 and CG2 motifs, and used these parameters to analyze the sliding trend of -1 and +1 nucleosomes around TSS of genes with NFR in yeast...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020002
更新日期：2020-05-01 00:00:00
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journal_title：General physiology and biophysics
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doi：10.4149/gpb_2016001
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