Abstract:
:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms were measured up to IMI concentration of 0.5 mmol/l. Due to electrostatic attraction, binding between the positively charged IMI and the negatively charged surfaces of PS membranes was augmented compared to binding to neutral PC membranes. After correction for electrostatic effects by means of the Gouy-Chapman theory, the binding isotherms were described both by surface partition coefficients and by binding parameters (association constants and binding capacities). It was confirmed that binding of IMI to model membranes is strongly affected by negatively charged phospholipids and that the binding is heterogeneous; in fact, weak surface adsorption and incorporation of the drug into the hydrophobic core of lipid bilayer can be seen and characterized. These results support the hypothesis suggesting that the lipid part of biological membranes plays a role in the mechanism of antidepressant action.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Fisar Z,Fuksová K,Velenovská Msubject
Has Abstractpub_date
2004-03-01 00:00:00pages
77-99issue
1eissn
0231-5882issn
1338-4325journal_volume
23pub_type
杂志文章abstract::Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been reported to exhibit tumor inhibitory effects on many types of tumor cells. Numerous studies have demonstrated that microRNAs (miRs) involve in the biological process of carcinogenesis by controlling expression of their target mRNAs to facilitate tum...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2019041
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journal_title:General physiology and biophysics
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abstract::The substantia gelatinosa of the trigeminal subnucleus caudalis has been considered to be an essential location for the transference of orofacial sensory signals. The co-localization of inhibitory and excitatory neurotransmitters in the same substantia gelatinosa (SG) neurons has demonstrated their essential part in t...
journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016008
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:1991-06-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017064
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2020022
更新日期:2020-09-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018030
更新日期:2019-01-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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doi:10.4149/gpb_2013058
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::Diabetes mellitus leads to bone disorders such as osteopenia and osteoporosis that can increase fracture risk. On the other hand, sodium tungstate is an inorganic compound which exerts anti-diabetic activity in experimental studies due to its suggested insulin-mimetic or antioxidant activity. Therefore this study was ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00