Abstract:
:Mitochondrial nucleoids (hereafter nucleoids) contain genetic information, mitochondrial DNA, prerequisite for mitochondrial functioning, particularly information required for mitochondrial electron transport. To understand nucleoid functioning, it is imperative to know its ultrastructure and dynamics in the context of the actual mitochondrial state. In this study, we document the internal structure, different positions of nucleoids inside the mitochondrial tube and their different morphology. The nucleoid cores appear in section as circular or slightly oval objects ranging from 50 to 100 nm in diameter. They are mainly located in the matrix between cristae inside the mitochondrial tube but they are also frequently found close to the inner mitochondrial surface. In tightly packed form, their interior exhibits sophisticated nucleoprotein regularity. The core surroundings form an electron-lucent thick layer which is probably partitioned into separate chambers. We suggest that the morphology of nucleoids mirrors the mode of energy production, glycolysis versus oxidative phosphorylation. The new high resolution transmission electron microscopy method enabled us to obtain morphological characteristics on yet unpublished level.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Prachař Jdoi
10.4149/gpb_2016008subject
Has Abstractpub_date
2016-07-01 00:00:00pages
273-86issue
3eissn
0231-5882issn
1338-4325journal_volume
35pub_type
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