Abstract：
:The effect of hydroxyl radicals generated by the FeSO4/H2O2 system on structural properties of proteins and membranes was studied in rat cardiac mitochondria and myofibrils. Exposure of mitochondria to 0.1 mmol/l FeSO4/EDTA plus 1 mmol/l H202 at 37 degrees C for 30 or 60 min caused conjugated diene formation, but it was not accompanied by accumulation of fluorescent lipid-protein conjugates. On the other hand, fluorescence measurements revealed radical-induced and time-dependent loss of tryptophans and production of bityrosines. Under the same conditions, the gradual decrease in tryptophan flurescence and increase in bityrosine formation was also observed in radical-treated myofibrils. These results suggest that *OH radicals can alter the mitochondrial and myofibrillar function via oxidation of amino acid residues and might be implicated in the pathogenesis of myocardial injury.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Babusíková E,Kaplán P,Lehotský J,Jesenák M,Dobrota Dsubject
Has Abstractpub_date
2004-09-01 00:00:00pages
327-35issue
3eissn
0231-5882issn
1338-4325journal_volume
23pub_type
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journal_title：General physiology and biophysics
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更新日期：1984-06-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1987-04-01 00:00:00
abstract：:Overconsumption of fructose increases the risk of nonalcoholic fatty liver disease (NAFLD), obesity and metabolic syndrome. NAFLD is currently one of the most common etiologies of chronic liver disease worldwide. The aim of the present study is to evaluate the hepatoprotective potential of zingerone against fructose-e...
journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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doi：
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journal_title：General physiology and biophysics
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doi：
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doi：
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journal_title：General physiology and biophysics
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doi：
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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