Abstract:
:Photon emissions were measured at ambient temperature (21°C) in complete darkness once per min from cultures of 10(6) cells during the 12 h following removal from 37°C. The energy of emission was about 10(-20) J/s/cell. Of 8 different cell lines, B16-BL6 (mouse melanoma cells) demonstrated the most conspicuous emission profile. Acridine orange and ethidium bromide indicated the membranes were intact with no indication of (trypan blue) cell necrosis. Treatments with EGF and ionomycin produced rapid early (first 3 h) increases in energy emission while glutamine-free, sodium azide and wortmanin-treated cells showed a general diminishment 3 to 9 h later. The results suggested the most probable origin of the photon emission was the plasma cell membrane. Measures from cells synchronized at the M- and S-phase supported this inference.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Dotta BT,Buckner CA,Cameron D,Lafrenie RF,Persinger MAdoi
10.4149/gpb_2011_03_301subject
Has Abstractpub_date
2011-09-01 00:00:00pages
301-9issue
3eissn
0231-5882issn
1338-4325journal_volume
30pub_type
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