Abstract:
:This study investigated time-dependent variations in the activities of adenosine deaminase (ADA), an adenosine-metabolizing enzyme, and myeloperoxidase (MPO), an oxidation reaction-catalyzing enzyme, in control and streptozotocin (STZ)-induced diabetic rat liver. The animals were sacrificed at six different times of day (1, 5, 9, 13, 17 and 21 hours after lights on - HALO). The hepatic activity of ADA did not change depending on the STZ treatment whereas MPO activity was significantly higher in the diabetics than in the controls. Hepatic ADA activity was dependent on the time of sacrifice with the lowest activity at 21 HALO and the highest activity at 5 HALO. Both enzyme activities failed to show any significant interaction between STZ treatment and time of sacrifice, which means that diabetes does not influence the 24 h pattern of these activities. Since MPO, a heme protein localized in the leukocytes, is involved in the killing of microorganisms, increased MPO activity in diabetic rat liver may reflect leukocyte infiltration secondary to diabetes. A reduction in ADA activity during the dark (activity/feeding) period will presumably lead to high concentrations of adenosine in the liver, possibly contributing to changes in some metabolic processes, such as glycogen turnover and oxygen supply.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Uluoglu C,Cimen B,Ozbey G,Karasu C,Durakoglugil DB,Gunes A,Turkozkan N,Zengil Hsubject
Has Abstractpub_date
2008-03-01 00:00:00pages
25-31issue
1eissn
0231-5882issn
1338-4325journal_volume
27pub_type
杂志文章abstract::In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potentia...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016057
更新日期:2017-07-01 00:00:00
abstract::The mechanisms underlying the renoprotective effect for remote limb ischemic preconditioning (rIPC) against renal ischemia/reperfusion injury need further elucidation. In our work, one hundred and twenty male Sprague Dawley rats were randomized into 3 groups; sham, I/R group (left renal 45 min ischemia) and rIPC (as I...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015035
更新日期:2016-01-01 00:00:00
abstract::The effects of both chronic hypoxia and acute intermittent hypoxia (AIH) on cardiovascular system are unclear. We designed this study to develop a rabbit model of hypertension by exposure to chronic hypobaric hypoxia (CHH) and to investigate the effects of AIH on hypertensive rabbits. Present study was performed in 13...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013070
更新日期:2014-01-01 00:00:00
abstract::Kinase-targeted therapy is a new and promising approach to disease treatment. However, some kinase inhibitors have been observed to cause an off-target adverse risk for skeletal system by influencing the growth of osteoblasts. It is known that the proliferation and differentiation of osteoblasts are essentially regula...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018030
更新日期:2019-01-01 00:00:00
abstract::Cochlear spiral ganglion neurons provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Serotonin plays a role in the response properties of central auditory neurons. However, knowledge about the role of serotonin in the peripheral auditory nervous system re...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013005
更新日期:2013-03-01 00:00:00
abstract::The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Arterial base deficit/excess (BD/E) is commonly used marker of metabolic acidosis in critically ill patients, but requires an arterial puncture and blood gas analysis. We hypothesized that serum bicarbonate (HCO3), which can be routinely obtained, strongly correlates with arterial BD/E and provides equivalent predicti...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (ΔΨm) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016021
更新日期:2017-04-01 00:00:00
abstract::Nucleosome sliding and nucleosome digestion are two main ways for regulating gene transcription. We constructed three characteristic parameters (CP) based on the information of CG0, CG1 and CG2 motifs, and used these parameters to analyze the sliding trend of -1 and +1 nucleosomes around TSS of genes with NFR in yeast...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2020002
更新日期:2020-05-01 00:00:00
abstract::The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb-2019057
更新日期:2020-03-01 00:00:00
abstract:UNLABELLED:The effect of melatonin (MLT) on the isolated rat heart was studied using the standard perfusion conditions (Langendorff preparation) and model of calcium paradox (Ca(2+)-paradox). Ca(2+)-paradox was induced by 1 minute perfusion with Ca(2+)-free Krebs-Henseleit (KH) solution and subsequent 20 minutes perfus...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::PC12 cells differentiated under the influence of the neuronal growth factor (NGF) serve as a model of both sympathetic neurons and chromaffin cells. NGF-induced differentiation critically depends on elevated intracellular calcium concentration. Main pathway for Ca²⁺ entry in excitable cells is represented by voltage-d...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2014045
更新日期:2015-04-01 00:00:00
abstract::Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Phosphorylation of P-glycoprotein (PGP) by some protein kinases may play an important role in the regulation of its drug transport activity, and may also be important for the development of multidrug resistance (MDR) phenotype. In the present study we investigated the expression of three groups of mitogen-activated pr...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2010_03_266
更新日期:2010-09-01 00:00:00
abstract::A patient with a strongly lesioned spinal cord, sub C5, relearned running, besides improving other movements, by an oscillator formation training (rhythmic, dynamic, stereotyped exercise). After 45 days of jumping on a springboard and other rhythm trainings, the patient was able to run 90 m in 41 s (7.9 km/h) (even 9....
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::The hydrolytic activity and interaction of acidic and neutral phospholipase A2 (PLA2) with large unilamellar liposomes treated with cobra venom cytotoxin Vc5 (CT Vc5) were studied to more fully understand the modulating effects of cationic membrane-active peptides on PLA2. Studies were done by fluorescence displacemen...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Physical exercise could cause muscle and tissue damage due to increase in the formation of free oxygen radicals during exercise. The aim of the present study was to investigate the effect of crocin on parameters associated with oxidative stress in recovery from acute swimming exercise in rats. Rats were divided into e...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015018
更新日期:2016-01-01 00:00:00
abstract::We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix[6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013024
更新日期:2013-06-01 00:00:00
abstract::The interactions between haematoporphyrin (HP) and bilayer lipid membranes (BLM) were studied. A weak effect of HP on BLM conductivity was observed at HP concentrations ranging between 10(-6) and 3 x 10(-5) mol/l. Modulus of elasticity in the direction normal to the membrane plane (E perpendicular) and dynamic viscosi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxid...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Charcot-Marie-Tooth disease (CMT) and related peripheral neuropathies are the most commonly inherited neurological disorders in humans, characterized by clinical and genetic heterogeneity. The most prevalent clinical entities belonging to this group of disorders are CMT type 1A (CMT1A) and hereditary neuropathy with l...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2011_04_379
更新日期:2011-12-01 00:00:00
abstract::Hyperglycemia impairs oxidative capacity in skeletal muscle. Muscle oxidative capacity is regulated by peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α). Transcutaneous carbon dioxide (CO2) enhances PGC-1α expression in skeletal muscle. Therefore, the aim of this study was to clarify the effects of...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018048
更新日期:2019-05-01 00:00:00
abstract::Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::It is currently believed that the protein folding rate is related to the protein structures and its amino acid sequence. However, few studies have been done on the problem that whether the protein folding rate is influenced by its corresponding mRNA sequence. In this paper, we analyzed the possible relationship betwee...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2011_02_154
更新日期:2011-06-01 00:00:00
abstract::Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Thermal inactivation of peroxidase (POD) in an extract of Picea omorika (Pancić) Purkyne needles initiated by heat treatment was studied. This is the first study of this kind on a conifer species. Non-linear regression analysis was applied on the inactivation rate data, combining Mitscherlich and Arrhenius equations, ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2009_01_78
更新日期:2009-03-01 00:00:00
abstract::Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the abil...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract::The role of vascular endothelial growth factor (VEGF) in chronic stress and neurodevelopmental disorders is of growing research interest. Here we show that post-weaning isolation rearing of rats decreased gene expression of VEGF in the hippocampus. Gene expression of VEGF upstream regulator fibroblast growth factor-2 ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2019019
更新日期:2019-07-01 00:00:00