Abstract:
:The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of the conformational transition of metmyoglobin-heparin complex equilibrated at neutral pH observed after pH change to acidic region comprises two steps. During the first step, characterized by rapid changes of the absorption spectra (approximately 5 minutes) as well as fluorescence intensities, reversible transition with pK = 6.5 +/- 0.1 occurs and the first type of the complex forms. Below pH 6.2 the transition with pK = 5.7 +/- 0.1 is observed and the second type of the complex is formed. During the second slow step, the third type of the complex formed after 30 minutes of equilibration is characterized by a spectrum corresponding to low-spin form without protein axial ligand bound. At neutral pH and 25 degrees C, the interaction between metMb and heparin only slightly alters absorption and fluorescence spectra. On the other hand, the formation of metMb-heparin complex is established from the decrease of the transition temperature from 80.4 +/- 0.5 degrees C to 74.7 +/- 0.5 degrees C. Moreover, the binding of heparin prevents the aggregation of the protein at isoelectric point resulting in a considerable increase in the reversibility of thermal denaturation.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Fedunová D,Antalík Msubject
Has Abstractpub_date
1998-06-01 00:00:00pages
117-31issue
2eissn
0231-5882issn
1338-4325journal_volume
17pub_type
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2009_04_371
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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