Abstract:
:Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A new mathematical description of the reverse osmosis is also utilized. The work is a starting point for further quantitative studies and simulations of the phloem transport of water and assimilates in plants.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Kargol M,Suchanek G,Kargol Asubject
Has Abstractpub_date
2001-06-01 00:00:00pages
191-202issue
2eissn
0231-5882issn
1338-4325journal_volume
20pub_type
杂志文章abstract::Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::The ionic composition of intracellular space is rigorously maintained in the expense of high-energy expenditure. It has been recently postulated that the cytoplasmic ionic composition is optimized so the energy cost of the fluctuations of calcium ion concentration is minimized. Specifically, thermodynamic arguments ha...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016029
更新日期:2017-04-01 00:00:00
abstract::It is currently believed that the protein folding rate is related to the protein structures and its amino acid sequence. However, few studies have been done on the problem that whether the protein folding rate is influenced by its corresponding mRNA sequence. In this paper, we analyzed the possible relationship betwee...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2011_02_154
更新日期:2011-06-01 00:00:00
abstract::Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Arterial base deficit/excess (BD/E) is commonly used marker of metabolic acidosis in critically ill patients, but requires an arterial puncture and blood gas analysis. We hypothesized that serum bicarbonate (HCO3), which can be routinely obtained, strongly correlates with arterial BD/E and provides equivalent predicti...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Many herbicides employed in agriculture and also some antibiotics bind to a specific site of the reaction centre protein (RC) blocking the photosynthetic electron transport. Crystal structures showed that all these compounds bind at the secondary ubiquinone (QB) site albeit to slightly different places. Different herb...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015003
更新日期:2015-04-01 00:00:00
abstract::Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::The distributions of K, Na, Mg and Ca within frog ovarian and oviductal oocytes were studied by electron probe wavelength dispersive X-ray microanalysis. An important heterogeneity could be found both in nuclear and jelly coated oocytes. The highest K, Mg and, to a lesser extent, Na concentrations were found in the pi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::Diabetic cardiomyopathy is a severe complication of diabetes mellitus (DM). The goal of current work was to study the effects of sinomenine on streptozotocin-induced cardiomyopathy in rats. DM in rats was induced by intraperitoneal injection of streptozotocin. Cardiac function was evaluated by measuring left ventricle...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016033
更新日期:2017-01-01 00:00:00
abstract::Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, gui...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013016
更新日期:2013-06-01 00:00:00
abstract::We studied the involvement of O2, pH and low molecular thiols in H2S-induced decomposition of S-nitrosoglutathione (GSNO). The GSNO decomposition - •NO release was evaluated by UV-VIS spectroscopy and Griess assay. The H2S donor Na2S was used. O2 slightly increased, but was not necessary for the H2S-induced GSNO decom...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013026
更新日期:2013-09-01 00:00:00
abstract::The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb-2019057
更新日期:2020-03-01 00:00:00
abstract::The effects of both chronic hypoxia and acute intermittent hypoxia (AIH) on cardiovascular system are unclear. We designed this study to develop a rabbit model of hypertension by exposure to chronic hypobaric hypoxia (CHH) and to investigate the effects of AIH on hypertensive rabbits. Present study was performed in 13...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013070
更新日期:2014-01-01 00:00:00
abstract::Lignin, the structural polymer of the plant cell walls, is produced by free radical polymerization of phenolic alcohols, catalyzed by different peroxidases. The mechanism and the structural organization of lignin in the cell have not been completely understood. In this study we applied fractal analysis to images of li...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The interactions between haematoporphyrin (HP) and bilayer lipid membranes (BLM) were studied. A weak effect of HP on BLM conductivity was observed at HP concentrations ranging between 10(-6) and 3 x 10(-5) mol/l. Modulus of elasticity in the direction normal to the membrane plane (E perpendicular) and dynamic viscosi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::The activity of different cathepsins and neutral proteinases was measured in normal and vitamin E-deficient rabbit muscles using specific substrates. Among the changes of enzyme activities in dystrophy caused by vitamin E-deficiency the increase in the activity of cathepsin B is the most striking. The activity of cath...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::Effects of haloperidol on L-type CaV1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV1.2a) and vascular (CaV1.2b) splice...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2009_03_249
更新日期:2009-09-01 00:00:00
abstract::Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::The aim of the present study was to investigate the ability of the chloroacetanilide herbicide acetochlor to interact with the endocrine system. The modulation of the binding of [3H]estradiol-17beta to protamine sulphate-precipitated uterine nuclear and cytoplasmic estrogen receptors was analysed for this purpose. Two...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Myosin was isolated from pig atrial and ventricular myocardium during postnatal development and Ca2+-ATPase was determined and myosin light chains were analysed by electrophoresis in sodium dodecylsulfate polyacrylamide gel. During ontogenesis ATPase activity of ventricular myosin remains virtually unchanged, whereas ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. Cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of Helix pomatia (diameter: 150 microns) served as the test cells. The l...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00