Abstract：
:The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically increased expression of PCSK9 induced by hypoxia/reoxygenation (H/R) stress rather than by apoptosis in primary murine cardiomyocytes and HL-1 cells. Moreover, PCSK9 promoted H/R-induced pro-inflammatory cytokine release from macrophages, while silencing of PCSK9 inhibited the expression of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. Additionally, PCSK9 facilitated the release of pro-inflammatory cytokines from macrophages under H/R conditions, which decreased cardiomyocyte viability and promoted apoptosis of cardiomyocytes. For the underlying mechanisms, we identified PCSK9-induced NF-κB activation as being involved in the cardiomyocyte apoptosis, which was blocked by the NF-κB inhibitor BAY 11-7082. Collectively, this study provides new insights into the therapeutic possibility of regulating PCSK9 in cardiomyocytes for the treatment of ischaemic cardiomyopathy.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Yang CL,Zeng YD,Hu ZX,Liang Hdoi
10.4149/gpb-2019057subject
Has Abstractpub_date
2020-03-01 00:00:00pages
123-134issue
2eissn
0231-5882issn
1338-4325journal_volume
39pub_type
杂志文章abstract：:A model of activation of muscle contraction has been proposed. It is based on calcium diffusion and binding to specific regulatory sites in a sarcomere. Calcium ions activate interactions of contractile proteins and thus the generation of force. The model quantifies the relation between calcium released from intracell...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:CRM197, cross-reacting material 197, is a mutant of diphtheria toxin (DTx). CRM197 is used in pharmacology as a carrier protein. It has been recently shown that CRM197 causes breakdown in actin filaments. In order to show intracellular localization of CRM197 and visualize cell structure via actin cytoskeleton, endothe...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017006
更新日期：2017-10-01 00:00:00
abstract：:Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_005
更新日期：2012-03-01 00:00:00
abstract：:Hyperbaric oxygen treatment (HBO) could transiently reverse hypoxia during acute myocardial infarction (AMI). In order to evaluate whether early HBO can identify viable segments after AMI, improvement of wall motion score index (WMSI) after HBO was compared to dobutamine stress echocardiography (DSE). Thirty-one patie...
journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. Cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of Helix pomatia (diameter: 150 microns) served as the test cells. The l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Homeostasis and development in vertebrates are regulated by cell proliferation, differentiation and death. Permeability of mitochondrial membranes, a decisive feature of apoptosis, is regulated by Bcl-2 family regulators. Protein p53 is able to reduce bcl-2 and promote bax expression. This study focused on the immunoh...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:This study was designed to investigate the possible effectiveness of chronic resveratrol administration on redox state, inflammatory mediators and apoptosis rate in diabetic rats. Male Wistar rats were divided into four groups (n = 6): normal control, diabetic control, normal rats treated with resveratrol, and diabeti...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_039
更新日期：2012-12-01 00:00:00
abstract：:Effects of haloperidol on L-type CaV1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV1.2a) and vascular (CaV1.2b) splice...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_03_249
更新日期：2009-09-01 00:00:00
abstract：:Many herbicides employed in agriculture and also some antibiotics bind to a specific site of the reaction centre protein (RC) blocking the photosynthetic electron transport. Crystal structures showed that all these compounds bind at the secondary ubiquinone (QB) site albeit to slightly different places. Different herb...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015003
更新日期：2015-04-01 00:00:00
abstract：:The hormone leptin, by binding to hypothalamic receptors, suppresses food intake and decreases body adiposity. Leptin receptors are also widely expressed in extra-hypothalamic areas such as hippocampus, amygdala and cerebellum, where leptin modulates synaptic transmission. Here we show that a defective leptin receptor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013056
更新日期：2014-01-01 00:00:00
abstract：:The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:The plasma membrane H(+)-ATPase of Neurospora has been reconstituted into planar lipid bilayer membranes by means of the vesicle-fusion technique described by Finkelstein and his collaborators (Zimmerberg et al., 1980; Cohen et al., 1980, 1984; Akabas et al., 1984). Enzyme was first transferred from isolated plasma me...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:This study specified the role of several key calcium-operating ion channels in contraction/relaxation of human detrusor muscle as possible target for overactive bladder (OAB) treatment. Detrusor samples, obtained from 18 males (average age 61.5 ± 5.9 years), were investigated by organ tissue bath method with following...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017064
更新日期：2018-07-01 00:00:00
abstract：:The relationship between erythrocyte shape and the critical cell volume was investigated. Agents able to increase the critical cell volume induced three main stable shapes of erythrocytes: discocytic, stomatocytic, and echinocytic. The absence of correlation between shape and critical cell volume under isoosmotic cond...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The purpose of this study was to assess the possible protective effects of Artemisia campestris against fenthion-induced nephrotoxicity in adult rats and their progeny. Fenthion was administered orally at a dose of 551 ppm, which represented ¼ of LD50, for 21 consecutive days to pregnant and lactating rats. Oxidative ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013047
更新日期：2013-12-01 00:00:00
abstract：:In the present study effects of a new local anaesthetics, pentacaine (trans-2-pyrolidinocyclohexylester of 3-pentyloxyphenylcarbamic acid), and of some chemically related compounds on rat hepatic adenylate cyclase activity were studied under various experimental conditions. As compared with tetracaine, the local anaes...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:The character of structural changes in thylakoid membranes caused by temperature variation was investigated. Experiments were performed on maize leaf segments in vivo and in a closed temperature cycle 24-50-24 degrees C. Two biophysical methods were used for detection: luminescence and EPR. Arrhenius plots of delayed ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Currents through DPI 201-106 modified single cardiac sodium channels in guinea pig ventricular cells were measured using the patch clamp technique in the cell-free configuration to control the sodium concentrations on both sides of the patch membrane. Current-voltage relationships of the single channels were obtained ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BamHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Environmental exposure to modern microwave telecommunication electromagnetic fields (EMFs) has increased to unprecedented levels with consequent health complaints and concerns. Many studies have already reported genotoxic effects on a variety of organisms and cell/tissue types. Human peripheral blood lymphocytes from ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019032
更新日期：2019-09-01 00:00:00
abstract：:The left and right ventricles fulfill different role in heart function. Here we compare chamber specific changes in local catecholamine concentrations; gene expression and the receptor protein amount of all three β-adrenoceptors (β-AR) in rat right heart ventricles exposed to acute (1 session) and repeated (7 sessions...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014002
更新日期：2014-01-01 00:00:00
abstract：:Limited numbers of studies include research of microvibration therapy in experimental models. We examined effects of chronic vibroacustic-microvibration treatment on haemodynamics and anti-oxidative defense in experimental hypertension. Study was performed on chronically treated hypertensive and normotensive Wistar ra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014033
更新日期：2015-01-01 00:00:00
abstract：:The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013077
更新日期：2014-01-01 00:00:00
abstract：:Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been reported to exhibit tumor inhibitory effects on many types of tumor cells. Numerous studies have demonstrated that microRNAs (miRs) involve in the biological process of carcinogenesis by controlling expression of their target mRNAs to facilitate tum...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019041
更新日期：2019-11-01 00:00:00
abstract：:In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017007
更新日期：2017-10-01 00:00:00
abstract：:The substantia gelatinosa of the trigeminal subnucleus caudalis has been considered to be an essential location for the transference of orofacial sensory signals. The co-localization of inhibitory and excitatory neurotransmitters in the same substantia gelatinosa (SG) neurons has demonstrated their essential part in t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019027
更新日期：2019-09-01 00:00:00
abstract：:Participation of electrostatic and other noncovalent interactions in the binding of tricyclic antidepressants (TCAs) to the lipid bilayers was estimated from pH-dependencies of imipramine, desipramine, amitriptyline and nortriptyline binding to the lipid bilayers prepared from different phospholipids, both electroneut...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
