Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain.

Abstract:

:The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility increased with increasing temperature and decreased with increasing salt concentration. The temperature-dependent solubility was used to characterize the thermodynamic parameters of crystallization such as enthalpy, entropy and free energy.

journal_name

Gen Physiol Biophys

authors

Borgi MA,Rhimi M,Kadri A

doi

10.4149/gpb_2013077

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

227-34

issue

2

eissn

0231-5882

issn

1338-4325

journal_volume

33

pub_type

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