Abstract：
:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zone and the outer part of the SR membrane was shown to increase with a rise in temperature from 4 to 44 degrees C, the temperature of 20 degrees C being critical for these changes. In the presence of ATP, critical changes in the spin probe mobility occur at lower temperatures, while in the presence of ATP and Ca2+ they are observed at 20 degrees C for a spin probe localized in the outer part of the SR membrane. The mobility of a spin probe localized in the hydrophobic part of the membrane increases linearly with a rise in temperature. In the absence of ligands, the segmental motion of Ca-ATPase changes linearly within a temperature range of 10-30 degrees C. However, when ATP alone or ATP and Ca2+ are simultaneously added to the incubation mixture, the protein mobility undergoes critical changes at 20 degrees C. The Arrhenius plots for ATPase activity and Ca2+ uptake rate in SR membrane preparations also have a break at 20 degrees C. It is assumed that changes in the structural state of membrane lipids produce conformational changes in the Ca-ATPase molecule; the enzyme seems to be unsensitive to the structural state of the membrane lipid matrix in the absence of the ligands.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Rubtsov AM,Sentjurc M,Schara Msubject
Has Abstractpub_date
1986-10-01 00:00:00pages
551-61issue
5eissn
0231-5882issn
1338-4325journal_volume
5pub_type
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
pub_type： 杂志文章
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更新日期：1993-04-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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更新日期：1992-04-01 00:00:00
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