Abstract:
:Participation of electrostatic and other noncovalent interactions in the binding of tricyclic antidepressants (TCAs) to the lipid bilayers was estimated from pH-dependencies of imipramine, desipramine, amitriptyline and nortriptyline binding to the lipid bilayers prepared from different phospholipids, both electroneutral and acidic. The binding was studied using a radioligand binding assay. It was found that the membrane phospholipid composition and methylation of the acyl side chain of TCA has a decisive effect on participation of particular noncovalent interactions in the binding. Apparent high-affinity binding of TCAs to the phosphatidylcholine or phosphatidylethanolamine membranes are achieved mainly by incorporation of uncharged drug molecules into the hydrophobic core of the bilayers. Van der Waals forces and hydrophobic effect are responsible for this binding. Both charged and uncharged drug molecules bind to phosphatidylserine membranes, therefore coulomb- or ion-induced dipole interactions play a role in these binding. Different spatial distribution of charged residues within the interface causes different electrostatic interactions between charged TCAs and vesicles formed from phosphatidylserine and phosphatidylinositol. The data supports the hypothesis under which TCAs could have effect on affective disorders partially via binding to the lipid part of the membrane and following changes of lipid-protein interactions.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Fisar Zsubject
Has Abstractpub_date
2005-06-01 00:00:00pages
161-80issue
2eissn
0231-5882issn
1338-4325journal_volume
24pub_type
杂志文章abstract::Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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更新日期:2009-01-01 00:00:00
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journal_title:General physiology and biophysics
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更新日期:1999-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
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更新日期:1999-09-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:1991-06-01 00:00:00
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journal_title:General physiology and biophysics
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更新日期:1997-03-01 00:00:00
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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更新日期:1996-08-01 00:00:00
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