Abstract：
:A number of computational methods for the description of the ligand-receptor interaction that were developed in the past decade are reviewed in this paper. The central two sections introduce the methods that are already established as useful tools for the qualitative and quantitative description of ligand-receptor complexes, either when the detailed atomic structure of the receptor is known or not. The following section gives two examples of the application of the described methodology on two limiting cases.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Hornák V,Dvorský R,Sturdík Esubject
Has Abstractpub_date
1999-09-01 00:00:00pages
231-48issue
3eissn
0231-5882issn
1338-4325journal_volume
18pub_type
杂志文章,评审abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:CRM197, cross-reacting material 197, is a mutant of diphtheria toxin (DTx). CRM197 is used in pharmacology as a carrier protein. It has been recently shown that CRM197 causes breakdown in actin filaments. In order to show intracellular localization of CRM197 and visualize cell structure via actin cytoskeleton, endothe...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017006
更新日期：2017-10-01 00:00:00
abstract：:The purpose of this study was to assess the possible protective effects of Artemisia campestris against fenthion-induced nephrotoxicity in adult rats and their progeny. Fenthion was administered orally at a dose of 551 ppm, which represented ¼ of LD50, for 21 consecutive days to pregnant and lactating rats. Oxidative ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013047
更新日期：2013-12-01 00:00:00
abstract：:A mechanical model of heart muscle is proposed which includes rheological equations and equations for Ca-troponin interaction, for the dependences of the number of myosin cross-bridges on the length of sarcomere and on the speed of motion. The main assumption of the model is the dependence of the troponin affinity to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potentia...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016057
更新日期：2017-07-01 00:00:00
abstract：:Limited numbers of studies include research of microvibration therapy in experimental models. We examined effects of chronic vibroacustic-microvibration treatment on haemodynamics and anti-oxidative defense in experimental hypertension. Study was performed on chronically treated hypertensive and normotensive Wistar ra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014033
更新日期：2015-01-01 00:00:00
abstract：:Recent findings suggest that polymorphisms in vitamin D pathway genes are candidates for association with multiple sclerosis susceptibility. It has been now well demonstrated that vitamin D has immunomodulatory functions that may be favorable for reduction of multiple sclerosis risk. Current research has been focused ...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2013067
更新日期：2013-12-01 00:00:00
abstract：:The mechanisms underlying the renoprotective effect for remote limb ischemic preconditioning (rIPC) against renal ischemia/reperfusion injury need further elucidation. In our work, one hundred and twenty male Sprague Dawley rats were randomized into 3 groups; sham, I/R group (left renal 45 min ischemia) and rIPC (as I...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015035
更新日期：2016-01-01 00:00:00
abstract：:The human pituitary tumor-transforming gene-1 (hPTTG1) has been found to be overexpressed in various cancers. Accumulated evidences implicate that some of protein kinases can specifically recognize two PXXP motifs at hPTTG1 C-terminus through their Src homology (SH3) domain and then phosphorylate the protein by their ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016035
更新日期：2017-01-01 00:00:00
abstract：:Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been reported to exhibit tumor inhibitory effects on many types of tumor cells. Numerous studies have demonstrated that microRNAs (miRs) involve in the biological process of carcinogenesis by controlling expression of their target mRNAs to facilitate tum...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019041
更新日期：2019-11-01 00:00:00
abstract：:It is well known that in vitro storage lesions lead to membrane dysfunction and decreased number of functional erythrocytes. As erythrocytes get older, in storage media as well as in peripheral circulation, they undergo a variety of biochemical changes. In our study, the erythrocytes with different age groups in citra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016001
更新日期：2016-07-01 00:00:00
abstract：:The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The distributions of K, Na, Mg and Ca within frog ovarian and oviductal oocytes were studied by electron probe wavelength dispersive X-ray microanalysis. An important heterogeneity could be found both in nuclear and jelly coated oocytes. The highest K, Mg and, to a lesser extent, Na concentrations were found in the pi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats recei...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:A number of studies have reported that male reproductive organs are susceptible to electromagnetic fields (EMFs). The aim of this study was to explore the effects of microwave radiation exposures on 6-8 weeks old male Swiss albino mice. Mice were divided into two groups: group I - sham-exposed (Control), and group II ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017059
更新日期：2018-07-01 00:00:00
abstract：:Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the abil...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:The substantia gelatinosa of the trigeminal subnucleus caudalis has been considered to be an essential location for the transference of orofacial sensory signals. The co-localization of inhibitory and excitatory neurotransmitters in the same substantia gelatinosa (SG) neurons has demonstrated their essential part in t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019027
更新日期：2019-09-01 00:00:00
abstract：:The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb-2019057
更新日期：2020-03-01 00:00:00
abstract：:The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The aim of present study was to examine the impact of prenatal ethanol exposure on seizure susceptibility of the offspring. Pregnant Wistar rats were compelled to drink either 10% or 20% ethanol solution, as the only drinking fluid since conception up to the weaning of their offspring at the age of 28 days. Pregnant a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_019
更新日期：2012-06-01 00:00:00
abstract：:Opioid mu-receptors are membrane bound receptors. The mechanism by which they transduce their biological effect into the inner compartment of the postsynaptic cell is still not fully understood. The present study was attempted to the measurement of changes of the thermodynamic parameters of the receptor--agonist/antag...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The voltage dependent ionic currents in cultured embryonic skeletal myocytes at stages of development ranging from 1 to 6 day were studied using the whole-cell patch clamp technique. Sodium (INa) and calcium (ICa) inward and potassium (IK) outward currents were observed at all stages. INa did not differ from that desc...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Culturing the fresh-water alga Hydrodictyon reticulatum for 9 days in the presence of 0.2 and 0.4 mmol/l Ba2+ increases the membrane potential about twice and the plasmalemma resistance 5-10 times without having any significant effect on the cell contents of water and Na+, K+ and Cl- ions. While in control cells the m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Kinase-targeted therapy is a new and promising approach to disease treatment. However, some kinase inhibitors have been observed to cause an off-target adverse risk for skeletal system by influencing the growth of osteoblasts. It is known that the proliferation and differentiation of osteoblasts are essentially regula...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018030
更新日期：2019-01-01 00:00:00
abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:125I-labelled o-iodobenzoate (OIB) was prepared by means of an isotopic exchange reaction; its distribution and excretion were determined and its pharmacokinetic parameters in rats were calculated. The calculated value of the half-life of OIB elimination was 38.7 +/- 0.7 min, the distribution volume was 278.2 +/- 53.5...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
