Abstract:
:The mechanisms underlying the renoprotective effect for remote limb ischemic preconditioning (rIPC) against renal ischemia/reperfusion injury need further elucidation. In our work, one hundred and twenty male Sprague Dawley rats were randomized into 3 groups; sham, I/R group (left renal 45 min ischemia) and rIPC (as I/R group with 3 cycles of left femoral ischemic PC just before renal ischemia). Rats were sacrificed at 2 h, 24 h, 48 h and 7 days. Serum creatinine and urea were measured at the baseline and endpoints. Also, histopathological examination and assessment of the expression of inflammatory cytokines e.g. TNF-α, IL-1β and ICAM-1 and antioxidant genes: Nrf2, HO-1 and NQO-1 and anti-apoptotic gene Bcl-2 in left kidney were done by the end of experiment. The results of this study demonstrated that, rIPC caused significant improvement in serum creatinine and BUN levels and in the expression of antioxidant genes and Bcl-2 antiapoptotic gene with significant attenuation of pro-inflammatory cytokines and histopathological damage score at all-time points compared to I/R group (p ≤ 0.05). In conclusion, inhibition of inflammatory cytokine (TNF-α, IL-1β and ICAM-1) formation and activation of antioxidant genes: Nrf2, HO-1 and NQO-1 and anti-apoptotic gene Bcl-2 could be possible underlying mechanisms for the renoprotective effect of rIPC.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Hussein AM,Harraz AM,Awadalla A,Barakat N,Khater S,Shokeir AAdoi
10.4149/gpb_2015035subject
Has Abstractpub_date
2016-01-01 00:00:00pages
77-86issue
1eissn
0231-5882issn
1338-4325journal_volume
35pub_type
杂志文章abstract::Malate dehydrogenases (MDH, L-malate:NAD oxidoreductase, EC 1.1.1.37), catalyze the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate/aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondria...
journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:2002-09-01 00:00:00
abstract::Lignin, the structural polymer of the plant cell walls, is produced by free radical polymerization of phenolic alcohols, catalyzed by different peroxidases. The mechanism and the structural organization of lignin in the cell have not been completely understood. In this study we applied fractal analysis to images of li...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The effect of hydroxyl radicals generated by the FeSO4/H2O2 system on structural properties of proteins and membranes was studied in rat cardiac mitochondria and myofibrils. Exposure of mitochondria to 0.1 mmol/l FeSO4/EDTA plus 1 mmol/l H202 at 37 degrees C for 30 or 60 min caused conjugated diene formation, but it w...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Hyperbaric oxygen treatment (HBO) could transiently reverse hypoxia during acute myocardial infarction (AMI). In order to evaluate whether early HBO can identify viable segments after AMI, improvement of wall motion score index (WMSI) after HBO was compared to dobutamine stress echocardiography (DSE). Thirty-one patie...
journal_title:General physiology and biophysics
pub_type: 临床试验,杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Many herbicides employed in agriculture and also some antibiotics bind to a specific site of the reaction centre protein (RC) blocking the photosynthetic electron transport. Crystal structures showed that all these compounds bind at the secondary ubiquinone (QB) site albeit to slightly different places. Different herb...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015003
更新日期:2015-04-01 00:00:00
abstract::The effects of different parts of extract from medicinal plant Conyza canadensis, used to control bleeding, on human blood platelet aggregation in vitro were investigated. Aqueous extract of Conyza c. from young or old plants, glycoconjugate part, polysaccharide part and aglycon part at the concentrations above 0.75 m...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Sleep is considered as a physiological regulator in the body. Gamma-aminobutyric acid (GABA) is a neurotransmitter that modulates sleep and affects cardiac functions. We evaluated effects of acute sleep deprivation (SD) on cardiac hemodynamic parameters, expression of pro-inflammatory cytokines, and Heat shock protein...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017049
更新日期:2018-05-01 00:00:00
abstract::Kinase-targeted therapy is a new and promising approach to disease treatment. However, some kinase inhibitors have been observed to cause an off-target adverse risk for skeletal system by influencing the growth of osteoblasts. It is known that the proliferation and differentiation of osteoblasts are essentially regula...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018030
更新日期:2019-01-01 00:00:00
abstract::The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphism...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017033
更新日期:2017-12-01 00:00:00
abstract::CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::The role of viscosity in coupling between chemical reaction (complex formation) and diffusion in membranes has been investigated. The Fick law was replaced by the momentum balance equation with the viscous term. The irreversible thermodynamics admits coupling of the chemical reaction rate with the gradient of velocity...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::The role of vascular endothelial growth factor (VEGF) in chronic stress and neurodevelopmental disorders is of growing research interest. Here we show that post-weaning isolation rearing of rats decreased gene expression of VEGF in the hippocampus. Gene expression of VEGF upstream regulator fibroblast growth factor-2 ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2019019
更新日期:2019-07-01 00:00:00
abstract::A model for the dynamics of quasi-1D lipid structures in biological membranes is proposed. The model takes into account interactions between the lipid heads and hydro-carbon chains, the description of their relaxation dynamics being based on the phenomenological Ginzburg-Landau approach. It is shown that in lateral li...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from intestinal L cells upon nutrients ingestion, and is currently used for treating diabetes mellitus. It plays an important role in receptor modulation and cross talk with insulin at the coronary endothelium (CE) and cardiomyocytes (CM) in diabetic type...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2011_01_34
更新日期:2011-03-01 00:00:00
abstract::Effects of haloperidol on L-type CaV1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV1.2a) and vascular (CaV1.2b) splice...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2009_03_249
更新日期:2009-09-01 00:00:00
abstract::Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2010_04_373
更新日期:2010-12-01 00:00:00
abstract::The human pituitary tumor-transforming gene-1 (hPTTG1) has been found to be overexpressed in various cancers. Accumulated evidences implicate that some of protein kinases can specifically recognize two PXXP motifs at hPTTG1 C-terminus through their Src homology (SH3) domain and then phosphorylate the protein by their ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016035
更新日期:2017-01-01 00:00:00
abstract::The character of structural changes in thylakoid membranes caused by temperature variation was investigated. Experiments were performed on maize leaf segments in vivo and in a closed temperature cycle 24-50-24 degrees C. Two biophysical methods were used for detection: luminescence and EPR. Arrhenius plots of delayed ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract::The activity of different cathepsins and neutral proteinases was measured in normal and vitamin E-deficient rabbit muscles using specific substrates. Among the changes of enzyme activities in dystrophy caused by vitamin E-deficiency the increase in the activity of cathepsin B is the most striking. The activity of cath...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The membrane lipid and fatty acid compositions of red blood cells from a paramyotonia patient were investigated. Cholesterol and total phospholipid contents in paramyotonia were not different from control. Only the sphingomyelin content was lower, and thus the molar ratio of phosphatidylcholine/sphingomyelin was highe...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::The existence of a high-conductance voltage sensitive chloride channel in BC3H1 myoblasts is documented. The conductance of the channel in symmetrical 150 mmol/l sodium chloride is around 400 pS. The probability of the channel being in the open state decreases with increasing of the imposed voltage from holding potent...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The ionic composition of intracellular space is rigorously maintained in the expense of high-energy expenditure. It has been recently postulated that the cytoplasmic ionic composition is optimized so the energy cost of the fluctuations of calcium ion concentration is minimized. Specifically, thermodynamic arguments ha...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016029
更新日期:2017-04-01 00:00:00
abstract::The effects of pentoxifylline (PTX) on intracellular accumulation of doxorobicin (DOX), DOX cytotoxicity and expression of Pgp in multidrug resistant L1210/VCR cell line were investigated. PTX (100 mg/l) was able to enhance the DOX accumulation in resistant cells. The maximum intracellular levels of DOX were reached a...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::Cochlear spiral ganglion neurons provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Serotonin plays a role in the response properties of central auditory neurons. However, knowledge about the role of serotonin in the peripheral auditory nervous system re...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013005
更新日期:2013-03-01 00:00:00
abstract::Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2020022
更新日期:2020-09-01 00:00:00