Abstract：
:The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphisms and breast cancer, to analyse the discriminative ability of each SNP and to test the accuracy of the predictive breast cancer risk model which includes all SNPs. We conducted a case-control study of 170 women (57.06 ± 11.60 years) with histologically confirmed breast cancer and 146 controls (50.24 ± 10.69 years). High resolution melting (HRM) method with Sanger sequencing validation was used in analyses. We have revealed significant association of FGFR2 and MAP3K1 polymorphisms with breast cancer. The odds ratio of FGFR2 T allele was 1.897 (95% CI 1.231-2.936, p = 0.004) and MAP3K1 C allele 1.804 (95% CI 1.151-2.845, p = 0.012). FGFR2 polymorphism achieved the best discriminative ability (41.95%). The Random Forest algorithm selected FGFR2, MAP3K1 and age as important breast cancer predictors. The accuracy of this prediction model approached moderate accuracy (70%), with 35.9% sensitivity and 88.6% specificity.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Dankova Z,Zubor P,Grendar M,Kapinova A,Zelinova K,Jagelkova M,Gondova A,Dokus K,Kalman M,Lasabova Z,Danko Jdoi
10.4149/gpb_2017033subject
Has Abstractpub_date
2017-12-01 00:00:00pages
565-572issue
5eissn
0231-5882issn
1338-4325journal_volume
36pub_type
杂志文章abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Beat-to-beat variability of action potential duration (short-term variability, SV) is an intrinsic property of mammalian myocardium. Since the majority of agents and interventions affecting SV may modify also action potential duration (APD), we propose here the concept of relative SV (RSV), where changes in SV are nor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015019
更新日期：2016-01-01 00:00:00
abstract：:The plasma membrane H(+)-ATPase of Neurospora has been reconstituted into planar lipid bilayer membranes by means of the vesicle-fusion technique described by Finkelstein and his collaborators (Zimmerberg et al., 1980; Cohen et al., 1980, 1984; Akabas et al., 1984). Enzyme was first transferred from isolated plasma me...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:In this study, we investigated the role of AMP-activated protein kinase (AMPK) and the importance of reactive oxygen species (ROS) in apoptosis induction associated with cell cycle arrest induced by sulforaphane in AGS human gastric cancer cells. Our results demonstrated that sulforaphane inhibited proliferation of AG...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017026
更新日期：2018-03-01 00:00:00
abstract：:The present results suggest that laminin-1 and 3 are localized in the specialized Schwann cells of Pacinian corpuscles, in spite of incomplete deposition of the basal lamina on the surface of their cytoplasmic processes. In addition, laminin-3 is concentrated and probably function as a stop protein not only in the neu...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Maternal milk is a significant source of hormones and other bioactive substances. They might be involved either in the control of mammary gland function or in the regulation of growth and development of the neonate. Atriopeptin (atrial natriuretic factor, ANF) is a peptide with strong diuretic, natriuretic and vasorel...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Opioid mu-receptors are membrane bound receptors. The mechanism by which they transduce their biological effect into the inner compartment of the postsynaptic cell is still not fully understood. The present study was attempted to the measurement of changes of the thermodynamic parameters of the receptor--agonist/antag...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017007
更新日期：2017-10-01 00:00:00
abstract：:The changes in mitochondrial membrane potential (Deltapsi(m)) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncou...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:Charcot-Marie-Tooth disease (CMT) and related peripheral neuropathies are the most commonly inherited neurological disorders in humans, characterized by clinical and genetic heterogeneity. The most prevalent clinical entities belonging to this group of disorders are CMT type 1A (CMT1A) and hereditary neuropathy with l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_04_379
更新日期：2011-12-01 00:00:00
abstract：:Aquaporin attracted attention not only of physiologists and biophysicists, but also of chemical engineers. Here we critically analyze a paper describing aquaporin-based artificial membranes, suggested for forward osmosis-based water purification (Wang et al. 2012, Small 8, pp. 1185-1190). Related papers published late...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
abstract：:The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:Elevated serum resistin is implicated in insulin resistance associated with obesity and type 2 diabetes mellitus. Alcohol consumption interferes with the nutritional status, metabolic and hormonal activity of the drinker. Impact of ethanol intake on resistin level and resistin metabolic effects is unknown. Effect of l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats recei...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_005
更新日期：2012-03-01 00:00:00
abstract：:The aim of the present study was to investigate the ability of the chloroacetanilide herbicide acetochlor to interact with the endocrine system. The modulation of the binding of [3H]estradiol-17beta to protamine sulphate-precipitated uterine nuclear and cytoplasmic estrogen receptors was analysed for this purpose. Two...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Effects of six thiol reagents with different physico-chemical properties were tested on the Na+-dependent 45Ca2+ transport into the rat brain microsomal membrane vesicles. The mercurials p-chlormercuribenzoate and Mersalyl effectively inhibited 45Ca2+ uptake with IC50 values in the order of 10(-4) mol X l-1 in the med...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:On the basis of values corresponding to concentrations exhibiting 50% inhibition of platelet aggregation induced with different stimuli and 50% inhibition of arachidonic acid liberation and thromboxane generation, we compared the antiplatelet effect of two cationic amphiphilic drugs--chloroquine and dithiaden. Compare...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Changes in the Young elasticity modulus in perpendicular direction to the membrane surface E perpendicular, in the coefficient of dynamic viscosity eta, in the electric capacitance C, in the surface charge U1, in the conductivity g and in the coefficient of non-linearity beta of current-voltage characteristic caused b...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020022
更新日期：2020-09-01 00:00:00
abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:Previously detected βsp and γ1sp dielectric relaxations on the spectrin-based membrane skeleton (MS) of human red blood cells (RBCs) have been shown sensitive to the attachment of MS to the lipid-protein membrane. Such relaxations were now detected on the MS of mammal (rat, horse, bovine, sheep and goat) and "unstrain...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020034
更新日期：2020-11-01 00:00:00
abstract：:Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017020
更新日期：2018-03-01 00:00:00
abstract：:Time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. Cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of Helix pomatia (diameter: 150 microns) served as the test cells. The l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:This study investigated time-dependent variations in the activities of adenosine deaminase (ADA), an adenosine-metabolizing enzyme, and myeloperoxidase (MPO), an oxidation reaction-catalyzing enzyme, in control and streptozotocin (STZ)-induced diabetic rat liver. The animals were sacrificed at six different times of d...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Participation of electrostatic and other noncovalent interactions in the binding of tricyclic antidepressants (TCAs) to the lipid bilayers was estimated from pH-dependencies of imipramine, desipramine, amitriptyline and nortriptyline binding to the lipid bilayers prepared from different phospholipids, both electroneut...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
