Abstract:
:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in CTC fluorescence indicating the release of membrane-bound Ca2+. The effect was independent of the level of extracellular Ca2+ and could be observed in the presence of EGTA; it was suppressed by the metabolic inhibitors FCCP, antimycin and sodium cyanide. Analysis of the excitation spectra of CTC fluorescence indicated that the observed effect is caused by redistribution of intracellular Ca2+ rather than Mg2+. Thus the lectin interaction with the lymphocyte plasma membrane results in Ca2+ release into the cytosol from the intracellular stores.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Gukovskaya AS,Zinchenko VP,Ryabichenko VV,Astashkin EIsubject
Has Abstractpub_date
1983-10-01 00:00:00pages
395-407issue
5eissn
0231-5882issn
1338-4325journal_volume
2pub_type
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